{"title":"Biosearch Technologies","description":"\u003cp\u003e\u003cb\u003eBiosearch Technologies has developed and supplied advanced diagnostics to support global healthcare. We offer end-to-end solutions from design to commercialisation, specialising in infectious diseases, oncology, prenatal testing, and more.\u003c\/b\u003e\u003c\/p\u003e","products":[{"product_id":"nxseq-adaptors-nxgseq-002","title":"NxSeq Adaptors","description":"\u003cp\u003eNxSeq Adaptors are used in combination with the NxSeq library preparation kits.\u003c\/p\u003e\u003cp\u003eEach box of NxSeq Adaptors contains (12) different indexed Illumina-compatible adaptors with enough of each adapter for 4 library reactions. Box 1 contains adaptors 1-12 and Box 2 contains adapters 13-24.\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eThe highest efficiency, low input, PCR-free fragment library prep kit available at the lowest cost.\u003c\/li\u003e\n\u003cli\u003eLow Input: Requires as little as 75 ng of sheared input DNA allowing use of limiting samples.\u003c\/li\u003e\n\u003cli\u003eHigh Efficiency: Optimised adaptor ligation produces more sequenceable fragments in each library, yielding better coverage \u0026amp; depth from single or multiplexed libraries.\u003c\/li\u003e\n\u003cli\u003ePCR-free: Prevents the introduction of PCR-bias, providing more uniform coverage.\u003c\/li\u003e\n\u003cli\u003eFast: 2 hour, 10 minute protocol saves you time and gets your samples on the sequencer sooner.\u003c\/li\u003e\n\u003cli\u003eAffordable: Best priced and best performing kit available.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eEach box of NxSeq Adaptors contains (12) different indexed, Illumina-compatible adaptors with enough of each adapter for 4 library reactions. Box 1 contains adaptors 1-12 and Box 2 contains adapters 13-24.\u003c\/p\u003e\n\u003ch2\u003eResources\u003c\/h2\u003e\n\u003cp\u003eBrochures and guides\u003c\/p\u003e\u003cp\u003ecase study mcgill nxseq ampfree\u003c\/p\u003e\u003cp\u003ePosters\u003c\/p\u003e\u003cp\u003eNxSeq Fragment Library AmpFREE Low Input PAG 2016\u003c\/p\u003e\n\u003ch2\u003eFAQs\u003c\/h2\u003e\n\u003cp\u003eFAQs\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_NXGSEQ-002_14300-1-NxSeq-Adaptors-Box-1\"\u003e14300 1 NxSeq Adaptors Box 1\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_NXGSEQ-002_14400-1-NxSeq-Adaptors-Box-2\"\u003e14400 1 NxSeq Adaptors Box 2\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_NXGSEQ-002_NxSeq-AmpFREE-Low-DNA-Library-Kit\"\u003eNxSeq AmpFREE Low DNA Library Kit\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/pis_NXGSEQ-002_F103-US-NxSeq-AmpFREE-Kit\"\u003eF103 US NxSeq AmpFREE Kit\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/guide_NXGSEQ-002_case-study-mcgill-nxseq-ampfree\"\u003ecase study mcgill nxseq ampfree\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/poster_NXGSEQ-002_NxSeq-Fragment-Library-AmpFREE-Low-Input-PAG-2016\"\u003eNxSeq Fragment Library AmpFREE Low Input PAG 2016\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"Box 1 (12 x 4 Reactions)","offer_id":45986428780623,"sku":"14300-1","price":1407.0,"currency_code":"ILS","in_stock":true},{"title":"Box 2 (12 x 4 Reactions)","offer_id":45986428813391,"sku":"14400-1","price":1407.0,"currency_code":"ILS","in_stock":true}]},{"product_id":"nxseq-ampfree-low-dna-library-kit-nxgseq-001","title":"NxSeq AmpFREE Low DNA Library Kit","description":"\u003cp\u003eYou’re doing next gen sequencing for a reason; you want big data! To get there, you know you need the best fragment libraries possible to get the most information out of each sample. So why spend your time, resources and expensive sequencing reagents on anything but the best fragment DNA libraries?\u003c\/p\u003e\u003cp\u003eThe NxSeq™ AmpFREE Low DNA Library Kit allows you to build the best fragment DNA libraries possible. We optimised each step of the protocol to ensure peak performance on Illumina sequencers. In addition, these kits require only 75 ng of sheared DNA input, and produce libraries in about 2 hours using a streamlined, easy to follow protocol.\u003c\/p\u003e\u003cp\u003eBetter Data\u003cbr\u003eHigher Efficiency Libraries\u003cbr\u003eMore Reads from Challenging FFPE Samples\u003cbr\u003eExcellent Sequencing Data Examples\u003cbr\u003eMinimal GC Bias\u003cbr\u003eFaster, Easier Protocol\u003cbr\u003eHigher Efficiency Libraries\u003c\/p\u003e\u003cp\u003eMore Sequenceable DNA Fragments per Library = More Data\u003c\/p\u003e\u003cp\u003eFigure 1. Percentage of library DNA with correctly ligated adaptors measured by qPCR. Duplicate libraries were prepped per kit\/organism (Human, Staphylococcus aureus, Rhodobacter sphaeroides (1 library only), and E. coli) according to the manufacturer’s recommended input amounts and protocols. Adaptor ligation efficiency was measured by qPCR using the KAPA Library Quantification Kit (Complete ROX Low, Item No.KK4873) and matching amplified library as an internal standard.\u003c\/p\u003e\u003cp\u003eSequencing Impact of Higher Efficiency Library Construction\u003c\/p\u003e\u003cp\u003eBetter Libraries Increase the Number of Reads per Library\u003c\/p\u003e\u003cp\u003eLibrary Kit\tDNA Input\tTotal Number of Sequencing Reads Per Library\u003cbr\u003eStaphylococcus aureus\u003cbr\u003e\tE. coli K12\u003cbr\u003eNxSeq AmpFREE Low DNA Library Kit\t75 ng\t5,649,946\t4,305,882\u003cbr\u003eKapa Hyper Prep Kit\t250 ng\t4,838,726 (-15%)\t1,647,452 (-62%)\u003cbr\u003eIllumina TruSeq DNA PCR-Free Library Prep Kit\t1 µg\t38,768 (-99%)\t1,543,558 (-64%)\u003c\/p\u003e\u003cp\u003eFigure 2a. Number of sequencing reads generated per library after multiplexing and running on a MiSeq Instrument. DNA fragment libraries were prepped in parallel for each kit\/organism according to the manufacturer’s recommended input amounts and protocols. Libraries were quantitated and normalized to 2 nM using the Bioanalyzer (size) and Qubit Fluorometer (amount). Equimolar amounts of each library were multiplexed and sequenced with a single MiSeq run using 2 ×150 bp chemistry. The number of sequencing reads obtained are shown as well as the percent reduction (%) in total reads compared to the appropriate NxSeq AmpFREE Kit results.\u003c\/p\u003e\u003cp\u003eMore Proof with Challenging FFPE Samples\u003c\/p\u003e\u003cp\u003eLibrary Kit\tSample Type\tInput Amount\tTotal Reads\tMapped Reads\u003cbr\u003e(repeat masked)\u003cbr\u003eNxSeq AmpFREE Low DNA Library Kit\tNormal gDNA\t75 ng\t2,163,636\t900,338\u003cbr\u003eFFPE DNA\t75 ng\t1,767,818\t688,074\u003cbr\u003eFFPE DNA\t150 ng\t1,706,714\t656,658\u003cbr\u003eKapa Hyper Prep Kit\tNormal gDNA\t250 ng\t1,567,276 (-28%)\t650,296 (-28%)\u003cbr\u003eFFPE DNA\t250 ng\t1,270,870 (-28%)\t487,872 (-29%)\u003c\/p\u003e\u003cp\u003eFigure 2b. Number of sequencing reads generated from matching normal and FFPE gDNA sample libraries. DNA fragment libraries were prepped using the two indicated kits according to the manufacturer’s recommended input amounts and protocols. Libraries were constructed from normal gDNA (Biochain, Cat. No. D1234142-S02) and DNA extracted from a matching FFPE human kidney tissue (Biochain Cat. No. T2234142-S02) using the Qiagen AllPrep DNA\/RNA FFPE Kit. The gDNA samples were sheared to ~250 bp before starting library construction. Final libraries were quantitated and normalized to 2 nM using the Bioanalyzer (size) and Qubit Fluorometer (amount). Equimolar amounts of each library were multiplexed and sequenced with a single MiSeq run using 2 × 150 bp chemistry. The number of sequencing reads obtained are shown as well as the percent reduction (%) in total and mapped reads compared to the corresponding NxSeq AmpFREE Kit results using 75 ng of input DNA.\u003c\/p\u003e\u003cp\u003eHighly Mappable Reads (\u0026gt;92%) from Human, Staphylococcus and Rhodobacter gDNA Sequencing\u003c\/p\u003e\u003cp\u003eSequencing Stat\u003c\/p\u003e\u003cp\u003eHuman\tStaphylococcus\u003c\/p\u003e\u003cp\u003eRhodobacter\u003c\/p\u003e\u003cp\u003eGenome size, GC percentage\u003c\/p\u003e\u003cp\u003e~3 Gbp 45% GC\u003c\/p\u003e\u003cp\u003e2,821,361 33% GC\u003c\/p\u003e\u003cp\u003e4,602,977 69% GC\u003c\/p\u003e\u003cp\u003eRaw reads\u003c\/p\u003e\u003cp\u003e3,131,114\u003c\/p\u003e\u003cp\u003e1,260,836\u003c\/p\u003e\u003cp\u003e3,900,174\u003c\/p\u003e\u003cp\u003eMapped reads\u003c\/p\u003e\u003cp\u003e2,979,237 (95.15%)\u003c\/p\u003e\u003cp\u003e1,174,111 (93.12%)\u003c\/p\u003e\u003cp\u003e3,613,165 (92.64%)\u003c\/p\u003e\u003cp\u003eRead length\u003c\/p\u003e\u003cp\u003e148.9 bp\u003c\/p\u003e\u003cp\u003e148.8 bp\u003c\/p\u003e\u003cp\u003e149.6 bp\u003c\/p\u003e\u003cp\u003eTotal bases\u003c\/p\u003e\u003cp\u003e443,767,447\u003c\/p\u003e\u003cp\u003e174,694,261\u003c\/p\u003e\u003cp\u003e540,403,552\u003c\/p\u003e\u003cp\u003eGenome fraction\u003c\/p\u003e\u003cp\u003e0.11\u003c\/p\u003e\u003cp\u003e0.97\u003c\/p\u003e\u003cp\u003e1.00\u003c\/p\u003e\u003cp\u003eAvg. coverage\u003c\/p\u003e\u003cp\u003e0.15X\u003c\/p\u003e\u003cp\u003e62X\u003c\/p\u003e\u003cp\u003e117X\u003c\/p\u003e\u003cp\u003eFigure 3. Representative gDNA sequencing stats from three different organisms. Genomic DNA fragment libraries were generated using the NxSeq AmpFREE Low DNA Library Kit using 75 ng of sheared gDNA input from three organisms (human, Staphylococcus aureus, and Rhodobacter sphaeroides). The final libraries were quantitated and normalized to 2 nM final concentrations using the Bioanalyzer and Qubit fluorometer, and 5 µL of each library was run on a MiSeq using 2 x 150 bp chemistry and analysed.\u003c\/p\u003e\u003cp\u003eMinimal Bias Detected\u003c\/p\u003e\u003cp\u003eFigure 4. Sequencing bias measured for three different organisms with varying GC content. DNA fragment libraries were generated from gDNA of three organisms with varying GC content ( Staphylococcus aureus, 24% GC; E. coli K12, 50% GC; and Rhodobacter sphaeroides, 68% GC) according to the manufacturer’s recommended input amounts and protocols. Samples were quantitated using the Bioanalyzer and Qubit fluorometer and normalized to 2 nM final concentrations. Five µL of each library sample was sequenced on a MiSeq using 2 x 150 bp v2 chemistry and analysed. Normalized coverage was calculated as the (average coverage of all windows with X% GC content) divided by the (overall average coverage).\u003c\/p\u003e\u003cp\u003eDon’t spend 25-74% More per Library with Another Kit\u003c\/p\u003e\u003cp\u003eSave Big with the NxSeq AmpFREE Low DNA Library Kit\u003c\/p\u003e\u003cp\u003ePCR-Free Fragment Library Kit\tCat. No.\tSize\u003cbr\u003e(rxns)\t2016 US\u003cbr\u003eList Price\tCost\/Reaction\tPer Rxn Cost Increase\u003cbr\u003evs. NxSeq Kit\u003cbr\u003eNxSeq\u003cbr\u003eAmpFREE Low\u003cbr\u003eDNA Library Kit\t14000-1\t12\t$240\t$20\u003cbr\u003e14000-2\t48\t$921\t$19\t12 rxn\t48 rxn\u003cbr\u003eKapa Hyper Prep Kit\tKK8501\t8\t$264\t$33\t+65%\t+74%\u003cbr\u003eKK8503\t24\t$696\t$29\t+45%\t+53%\u003cbr\u003eKK8505\t96\t$2400\t$25\t+25%\t+32%\u003cbr\u003eFaster Protocol with Significantly Less Hands-on Time\u003c\/p\u003e\u003cp\u003eSave Time and Get Your Samples on the Sequencer Sooner!\u003c\/p\u003e\u003cp\u003eread more\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eLow Input: Requires as little as 75 ng of sheared input DNA allowing use of limiting samples.\u003c\/li\u003e\n\u003cli\u003eHigh Efficiency: Optimised adaptor ligation produces more sequenceable fragments in each library, yielding better coverage \u0026amp; depth from single or multiplexed libraries.\u003c\/li\u003e\n\u003cli\u003ePCR-free: Prevents the introduction of PCR-bias, providing more uniform coverage.\u003c\/li\u003e\n\u003cli\u003eFast: 2 hour, 10 minute protocol saves you time and gets your samples on the sequencer sooner.\u003c\/li\u003e\n\u003cli\u003eAffordable: Best priced and best performing kit available.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eThe NxSeq AmpFREE Low DNA Library Kit is compatible with our NxSeq Adaptors or other dT-tailed, Illumina-compatible adaptors.\u003c\/p\u003e\u003cp\u003eEach NxSeq AmpFREE Low DNA Library Kit contains Enzyme Mix (EM), 2X Buffer (2XB), Ligase (LIG) and Elution Buffer (EB). Adaptors must be purchased separately.\u003c\/p\u003e\n\u003ch2\u003eResources\u003c\/h2\u003e\n\u003cp\u003eBrochures and guides\u003c\/p\u003e\u003cp\u003ecase study mcgill nxseq ampfree\u003c\/p\u003e\u003cp\u003ePosters\u003c\/p\u003e\u003cp\u003eNxSeq Fragment Library AmpFREE Low Input PAG 2016\u003c\/p\u003e\n\u003ch2\u003eFAQs\u003c\/h2\u003e\n\u003cp\u003eFAQs\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_NXGSEQ-001_SDS-001-14000-14300-1-14400-1-NxSeq-AmpFREE-Low-DNA-Library-Kit\"\u003eSDS 001 14000 14300 1 14400 1 NxSeq AmpFREE Low DNA Library Kit\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_NXGSEQ-001_SDS-14000-NxSeq-AmpFREE-Low-DNA-Library-Kit\"\u003eSDS 14000 NxSeq AmpFREE Low DNA Library Kit\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_NXGSEQ-001_NxSeq-AmpFREE-Low-DNA-Library-Kit\"\u003eNxSeq AmpFREE Low DNA Library Kit\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_NXGSEQ-001_NxSeq-UltraLow-DNA-Library-Kit-Quick-Protocol\"\u003eNxSeq UltraLow DNA Library Kit Quick Protocol\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/pis_NXGSEQ-001_F103-US-NxSeq-AmpFREE-Kit\"\u003eF103 US NxSeq AmpFREE Kit\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/guide_NXGSEQ-001_case-study-mcgill-nxseq-ampfree\"\u003ecase study mcgill nxseq ampfree\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/poster_NXGSEQ-001_NxSeq-Fragment-Library-AmpFREE-Low-Input-PAG-2016\"\u003eNxSeq Fragment Library AmpFREE Low Input PAG 2016\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"12 Reactions","offer_id":45986428878927,"sku":"14000-1","price":1936.0,"currency_code":"ILS","in_stock":true},{"title":"48 Reactions","offer_id":45986428911695,"sku":"14000-2","price":7357.0,"currency_code":"ILS","in_stock":true},{"title":"fragment","offer_id":45986428944463,"sku":"FRAG","price":710.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/NxSeq-AmpFREE-Low-DNA-Library-Kit_e20534fc-a72c-42f9-947a-df7235099132.jpg?v=1785956067"},{"product_id":"nxseq-single-indexing-kits-nxgseq-004","title":"NxSeq Single Indexing Kits","description":"\u003cp\u003eIllumina-compatible single indexing kits optimised for use with the NxSeq Ultralow DNA Library Kit.\u003c\/p\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eEach NxSeq Single Indexing Kit contains a Universal Adaptor and (12) different Primer Indexing Mixes with enough Universal Adaptor for 48 libraries and enough of each Primer Indexing Mix for 4 library amplification reactions (48 total reactions for all primer sets). Set A contains Primer Indexing Mixes 1-12 and Set B contains Primer Indexing Mixes 13-16, 18-23, 25, and 27. Each NxSeq Single Index equals the TruSeq® LT Index with the same number.\u003c\/p\u003e\n\u003ch2\u003eFAQs\u003c\/h2\u003e\n\u003cp\u003eFAQs\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_NXGSEQ-004_15100-1-NxSeq-Single-Indexing-Kit-Set-A\"\u003e15100 1 NxSeq Single Indexing Kit Set A\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_NXGSEQ-004_15200-1-NxSeq-Single-Indexing-Kit-Set-B\"\u003e15200 1 NxSeq Single Indexing Kit Set B\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_NXGSEQ-004_nxseq-ultralow-dna-library-kit-v2-12-reactions\"\u003enxseq ultralow dna library kit v2 12 reactions\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_NXGSEQ-004_nxseq-ultralow-dna-library-kit-v2-96-reactions\"\u003enxseq ultralow dna library kit v2 96 reactions\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/pis_NXGSEQ-004_data-sheet-nxseq-ultralow-dna-library-kits-v2\"\u003edata sheet nxseq ultralow dna library kits v2\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"Set A (12 x 4 Reactions)","offer_id":45986428977231,"sku":"15100-1","price":1484.0,"currency_code":"ILS","in_stock":true},{"title":"Set B (12 x 4 Reactions)","offer_id":45986429009999,"sku":"15200-1","price":1484.0,"currency_code":"ILS","in_stock":true}]},{"product_id":"nxseq-ht-dual-indexing-kit-15300-1","title":"NxSeq HT Dual Indexing Kit","description":"\u003cp\u003eMany next generation DNA sequencing applications or sample types require the construction of PCR-amplified DNA fragment libraries. (E.g. whole genome sequencing or resequencing from limiting genomic DNA amounts or FFPE and cell-free DNA samples, exome sequencing, ChIP-seq, etc.). To get the highest quality data for these applications and sample types, you need a DNA library preparation kit with the highest efficiency adaptor ligation followed by unbiased PCR amplification to produce the most complex, unbiased libraries possible. These high quality libraries produce the high quality sequencing data with uniform depth and minimal zero coverage needed to drive your NGS experiments forward.\u003c\/p\u003e\u003cp\u003eThe updated NxSeq™ UltraLow DNA Library Kits v2 and NxSeq Indexing Kits allow you to build high quality DNA fragment libraries from extremely low DNA input amounts – as low as 50 pg depending on sample type and Illumina sequencer used. If you have more DNA, no problem; you can use as much as 100 ng of input DNA with this system.\u003c\/p\u003e\u003cp\u003eTo generate these high quality libraries, we optimised each step of the protocol to ensure peak performance on Illumina sequencers. To help with the initial steps of DNA fragmentation, we have included guidance for mechanical shearing on an instrument like a Covaris LE220 and an optimised protocol for enzymatic fragmentation using dsDNA Shearase Plus from Zymo Research. In this updated kit, we replaced the PCR master mix with an improved master mix that performs better by decreasing bias across high AT-rich genomic regions. Not only will you produce high quality libraries, but also once you have your fragmented DNA, library prep is quick and easy - about three total hours.\u003c\/p\u003e\u003cp\u003eApplications\u003c\/p\u003e\u003cp\u003eThese library and indexing kits are compatible with multiple applications and sample types including whole genome sequencing (WGS) and resequencing (WGR) applications for genomic assembly, SNV\/mutation or copy number variation (CNV) identification. The system is also compatible with other NGS applications such as ChIP-seq and exome-seq. It also works well with both high quality input DNA samples as well as challenging sample types such as metagenomic, FFPE and cell-free DNA samples.\u003c\/p\u003e\u003cp\u003eFormats\u003c\/p\u003e\u003cp\u003eWe offer 12 and 96 reaction kits with protocols to match both low throughput, tube-based library prep and high throughput, 96-well plate-based library prep. To complement these kits, we offer both single indexing and combinatorial dual indexing kits. If you are interested in using different indexing systems such as unique dual indexes or higher numbers of combinatorial dual indexes, we can help by providing you with information on the design of our indexing primers. With that information, you can order custom indexing primers that work well with our universal adaptor (Figure. 1 below). Please contact us for more information.\u003c\/p\u003e\u003cp\u003eNxSeq UltraLow DNA Library Kit single index library construction\u003c\/p\u003e\u003cp\u003eFigure 1. Mechanics of NxSeq UltraLow Kit library construction. This workflow figure illustrates how DNA fragment libraries are constructed using the NxSeq UltraLow DNA Library Kit v2 and NxSeq Single Indexing Kits. Dual indexed construction is the same, except both i5 and i7 indexes are incorporated during the PCR amplification step. Note that some PCR fragments and products are not shown to simplify the illustration.\u003c\/p\u003e\u003cp\u003eFast, streamlined library preparation with minimal components required\u003c\/p\u003e\u003cp\u003eFigure 2. Time course for the NxSeq UltraLow DNA Library Kit v2 protocol. This figure illustrates the various steps and time required for each and total overall time. Please note that only three enzyme components are provided and required to complete library preparation, greatly simplifying library prep while reducing the risk of any errors.\u003c\/p\u003e\u003cp\u003eSetting the stage for success with the high efficiency adaptor ligation\u003c\/p\u003e\u003cp\u003eFigure 3. Efficiency of adaptor ligation to DNA library fragments. Two independent sets of libraries were prepped per kit\/organism using manufacturer’s recommended protocols and 1 ng of the same sheared genomic DNA input per library. Briefly, DNA fragments with adaptors ligated to both ends were measured using triplicate qPCR assays and an universal qPCR primer set, designed by Biosearch Technologies, that binds to and amplifies all adaptor-ligated DNA fragments independent of the kit used. Efficiency was determined by comparing the qPCR quantitation to fluorescence DNA quantitation. Efficiency data was averaged and then normalised to the corresponding NxSeq UltraLow Library Kit data (1.0) and plotted.\u003c\/p\u003e\u003cp\u003eConsistent, high quality sequencing data from 50 pg to 100 ng of genomic DNA\u003c\/p\u003e\u003cp\u003eTable 1. Sequencing data from DNA fragment libraries generated from 50 pg to 100 ng of sheared E. coli gDNA. Mechanically sheared (Covaris LE220, peak size 300 bp) E. coli genomic DNA was serially diluted and used to make triplicate libraries starting with the indicated gDNA input amounts and number of PCR amplification cycles using the NxSeq UltraLow DNA Library Kit v2 and a NxSeq Single Indexing Kit. The final libraries were quantitated and diluted to 2 nM based on Bioanalyzer (size) and fluorescence-based DNA quantitation. The diluted libraries were then pooled and sequenced on a MiSeq using 2 x 150 bp chemistry, and the average data from the triplicate libraries are shown.\u003c\/p\u003e\u003cp\u003e2A. Improved depth with less variation across three bacterial strains\u003cbr\u003e2B. Better coverage – both fewer zero coverage regions and total zero coverage\u003c\/p\u003e\u003cp\u003eTable 2. Sequencing data from DNA fragment libraries generated from three bacterial species with varied genomic DNA GC content. Genomic DNA from S. aureus, E. coli, and R. azotoformans were mechanically sheared (Covaris LE220) to a peak size ~350 bp. Five ng of each sheared gDNA was used to create triplicate DNA fragment libraries using the NxSeq UltraLow Kit v2, and two other supplier kits according to manufacturer’s recommendations. Based on each user manual, the NxSeq libraries were amplified with 8 PCR cycles and Supplier A and B were amplified with 10 and 8 cycles, respectively. The final libraries were pooled and sequenced on a MiSeq using 2 x 150 bp chemistry. Equal numbers of mapped reads were analysed per library, and the average data from the triplicate libraries is shown. Table 2A compares the sequencing depth data from the three kits while Table 2B compares the zero coverage metrics.\u003c\/p\u003e\u003cp\u003eLess GC- and AT-bias compared to other DNA fragment library kits\u003c\/p\u003e\u003cp\u003eFigure 4. Sequence bias analysis for three different organisms with varying GC content. Triplicate genomic DNA fragment libraries were generated from gDNA of three organisms with varying GC content using the NxSeq UltraLow DNA Library Kit v2, Supplier A Kit or Supplier B Kit according to the manufacturer’s recommended protocols using 5 ng aliquots of the same mechanically sheared genomic DNA samples. The final libraries were pooled and sequenced on a MiSeq using 2 × 150 bp chemistry. Normalized coverage was calculated as the (average coverage of all windows with x% GC content) divided by the (overall average coverage) and the data from each set of replicates was averaged and presented. The grey shaded area represents the percent of each genome with the indicated GC content.\u003c\/p\u003e\u003cp\u003eHighly consistent maize and human WGS results independent of gDNA input amount\u003c\/p\u003e\u003cp\u003eA. Human results\u003c\/p\u003e\u003cp\u003eB. Maize results\u003c\/p\u003e\u003cp\u003eTable 3. Sequencing data from DNA fragment libraries generated from human (A) and maize (B) genomic DNA. Genomic DNA from human and maize B73 were mechanically sheared (Covaris LE220) to a peak size ~350 bp). Ten and 100 ng of each sheared gDNA was used to build triplicate DNA fragment libraries using the NxSeq UltraLow DNA Library Kit v2 as outlined in the user manual. The 10 and 100 ng libraries were amplified with 7 and 5 PCR cycles respectively. The final libraries were pooled and sequenced on a NovaSeq using 2 x 150 chemistry. Equal numbers of mapped reads (200M) were analysed per library, and the average data from the triplicate libraries shown.\u003c\/p\u003e\u003cp\u003eLess GC- and AT-bias in maize libraries compared to other library kits\u003c\/p\u003e\u003cp\u003eFigure 5 Sequence bias analysis of maize libraries constructed with three library preparation kits. Triplicate genomic DNA fragment libraries were generated from maize B73 gDNA using the NxSeq UltraLow DNA Library Kit v2, Supplier A Kit or Supplier B Kit according to the manufacturer’s recommended protocols using 10 ng aliquots of the same mechanically sheared genomic DNA sample. The final libraries were pooled and sequenced on a NovaSeq using 2 × 150 chemistry. Normalised coverage was calculated as the (average coverage of all windows with x% GC content) divided by the (overall average coverage) and the data from each set of replicates was averaged and presented. The grey shaded area represents the percent of the maize genome with the indicated GC content.\u003c\/p\u003e\u003cp\u003eColoured dual indexing primer sets provide visual verification of correct primer dispensing\u003c\/p\u003e\u003cp\u003eFigure 6. 96-well PCR plate NxSeq HT Dual Indexing Kit primer dispensing protocol. The 700 Series Indexing Primers in the NxSeq HT Dual Indexing Kit contain an inert blue dye and the 500 Series Indexing Primers contain an inert yellow\/orange dye. The 700 Series Primers are dispensed first, row by row. As primers are dispensed, the rows become blue. The 500 Series Primers are then dispensed column by column, and as they are added, the well colour changes from blue to green. After dispensing is complete, all wells should exhibit a consistent green colour. The presence of clear, yellow\/orange or blue wells indicates that those wells did not get the appropriate 700 and\/or 500 Series Primers.\u003c\/p\u003e\u003cp\u003eread more\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\u003cli\u003eReagent colour coding system for convenient dispensing\u003c\/li\u003e\u003c\/ul\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eThe NxSeq HT Dual Indexing Kit contains a Universal Adaptor, Adaptor Dilution Buffer and Indexing Primers 701 – 712, and 501 – 508. There is enough of each reagent in this kit to generate 96 different combinatorial dual indexed libraries. Each NxSeq HT Dual Index equals the TruSeq® HT Index with the same number.\u003c\/p\u003e\u003cp\u003eThe NxSeq UltraLow DNA Library Kits v2 and NxSeq Indexing Kits are only compatible with Illumina sequencers\u003c\/p\u003e\n\u003ch2\u003eFAQs\u003c\/h2\u003e\n\u003cp\u003eFAQs\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_15300-1_SDS-15300-1-NxSeq-HT-Dual-Indexing-Kit\"\u003eSDS 15300 1 NxSeq HT Dual Indexing Kit\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_15300-1_nxseq-ultralow-dna-library-kit-v2-12-reactions\"\u003enxseq ultralow dna library kit v2 12 reactions\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_15300-1_nxseq-ultralow-dna-library-kit-v2-96-reactions\"\u003enxseq ultralow dna library kit v2 96 reactions\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/pis_15300-1_data-sheet-nxseq-ultralow-dna-library-kits-v2\"\u003edata sheet nxseq ultralow dna library kits v2\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"Default Title","offer_id":45986429108303,"sku":"15300-1","price":3487.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/NxSeq-HT-Dual-Indexing-Kit_b8c45e4b-c7e0-429d-a441-b60991c8ae41.jpg?v=1785956068"},{"product_id":"bst-dna-polymerase-exonuclease-minus-pcramp-001","title":"Bst DNA Polymerase, Exonuclease Minus","description":"\u003cp\u003eBst DNA Polymerase, Exonuclease Minus, is a recombinant form of the 67 kDa Bacillus stearothermophilus DNA Polymerase protein (large fragment). The enzyme has 5’-3’ polymerase activity and strand displacement activity, but it lacks 3’-5’ exonuclease activity. It also has reverse transcription activity.\u003c\/p\u003e\u003cp\u003eBst DNA Polymerase, Exonuclease Minus, has higher strand displacement activity than that of other suppliers (Figure 1). The enzyme can be used in nucleic acid amplification methods* such as isothermal amplification, whole genome amplification (WGA), and multiple displacement amplification (MDA). It also can be used in Next Generation sequencing.\u003c\/p\u003e\u003cp\u003eApplications\u003c\/p\u003e\u003cp\u003eStrand displacement amplification\u003cbr\u003eDNA sequencing through high GC regions (1,2)\u003cbr\u003eRapid sequencing from nanogram amounts of DNA template (3)\u003c\/p\u003e\u003cp\u003eThis enzyme has optimal activity at 65°C. It is suitable for sequencing DNA with high GC content and secondary structures. It is available in concentrations of 8,000 U\/ml or 50,000 U\/ml.\u003c\/p\u003e\u003cp\u003eBst DNA Polymerase, Exonuclease Minus, is supplied with 10X DNA Polymerase Buffer B, composed of 200 mM Tris-HCl pH 8.8, 100 mM (NH 4) 2SO 4, 100 mM KCl, 20 mM MgSO 4, and 1.0 % Triton X-100.\u003c\/p\u003e\u003cp\u003eFigure 1. Bst DNA Polymerase, Exonuclease Minus, possesses greater strand-displacing polymerase activity. M13 single stranded DNA was incubated with or without 8 units of Bst DNA Polymerase(+\/- Bst) in reaction buffer supplied by the manufacturer, with or without replication primer (+\/- primer) for 30 minutes at 65°C. MW, 1 kb ladder.\u003c\/p\u003e\u003cp\u003eTips for use:\u003c\/p\u003e\u003cp\u003eRequires 0.1% Triton X-100 for long term storage.\u003cbr\u003eReaction temperatures above 70°C are not recommended.\u003cbr\u003eBst DNA Polymerase cannot be used for thermal cycle sequencing.\u003c\/p\u003e\u003cp\u003eHeat Inactivation: 80ºC for 20 min.\u003c\/p\u003e\u003cp\u003eReferences:\u003c\/p\u003e\u003cp\u003eGriffin, H. and Griffin, A. (1994) PCR Technology, 228-229.\u003cbr\u003eMcClary, J. et al. (1991) J. DNA Sequencing and Mapping, 1, 173-180.\u003cbr\u003eMead, D.A. et al. (1991) Biotechniques, 11, 76-87.\u003c\/p\u003e\u003cp\u003eread more\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eHigh strand displacement activity.\u003c\/li\u003e\n\u003cli\u003eUsed in isothermal amplification and Next Generation Sequencing.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cp\u003eage Buffer:\u003c\/p\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eBst DNA Polymerase, Exonuclease Minus is available in two concentrations, 8,000 U\/ml and 50,000 U\/ml, in a storage buffer of 10 mM Tris-HCl, pH 7.5, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.1% Triton X-100, and 50% Glycerol. Also supplied is 10X DNA Polymerase Buffer B, composed of 200 mM Tris-HCl pH 8.8, 100 mM (NH4)2SO4, 100 mM KCl, 20 mM MgSO4,  and 1.0 % Triton X-100.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-001_30027-Bst-DNA-Polymerase-Exonuclease-Minus\"\u003e30027 Bst DNA Polymerase Exonuclease Minus\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-001_30028-1-Bst-DNA-Polymerase-Exonuclease-Minus-50000U-per-mL\"\u003e30028 1 Bst DNA Polymerase Exonuclease Minus 50000U per mL\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-001_SDS-30027-Bst-DNA-Polymerase-Exonuclease-Minus\"\u003eSDS 30027 Bst DNA Polymerase Exonuclease Minus\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-001_SDS-30028-1-Bst-DNA-Polymerase-Exonuclease-Minus-50000U-per-mL\"\u003eSDS 30028 1 Bst DNA Polymerase Exonuclease Minus 50000U per mL\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_PCRAMP-001_Bst-DNA-Polymerase-50-U\"\u003eBst DNA Polymerase 50 U\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_PCRAMP-001_Bst-DNA-Polymerase-8-U\"\u003eBst DNA Polymerase 8 U\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/pis_PCRAMP-001_Enzyme-Catalog-Full\"\u003eEnzyme Catalog Full\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"Exonuclease Minus 8,000 U\/mL, 2,000 U","offer_id":45986429141071,"sku":"30027-1","price":660.0,"currency_code":"ILS","in_stock":true},{"title":"Exonuclease Minus Kit, 40,000 U at 8,000 U\/mL","offer_id":45986429173839,"sku":"30027-3","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"Exonuclease Minus 50,000 U\/mL, 10,000 U","offer_id":45986429206607,"sku":"30028-1","price":2616.0,"currency_code":"ILS","in_stock":true},{"title":"Exonuclease Minus,  8,000 U\/mL, 40,000 U","offer_id":45986429239375,"sku":"F93635-2","price":9293.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/Bst-DNA-Polymerase_252C-Exonuclease-Minus_fb12678e-04a1-4b90-bd76-50e88d8673b0.jpg?v=1785956068"},{"product_id":"econotaq-dna-polymerase-pcramp-003","title":"EconoTaq DNA Polymerase","description":"\u003cp\u003eQC specifications for EconoTaq DNA Polymerase are rigorous:\u003c\/p\u003e\u003cp\u003eGreater than 99% pure by SDS gel electrophoresis.\u003cbr\u003eNo detectable DNA contamination as determined by PCR using generic primers.\u003cbr\u003eNo detectable endonuclease (nicking) activity. Incubation of 10 U of EconoTaq DNA Polymerase with 1 µg of supercoiled pBR322 DNA for 16 hours at 70 °C results in no detectable conversion to relaxed or linear forms by agarose gel electrophoresis.\u003cbr\u003eNo detectable exonuclease activity. Incubation of 10 U of EconoTaq DNA Polymerase with 1 µg of Hind III-cut lambda DNA for 16 hours at 70°C results in no smearing of bands on agarose gels.\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eChoice of reaction buffers, with or without MgCl2.\u003c\/li\u003e\n\u003cli\u003eNon-proofreading Polymerase\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cp\u003eConcentration: 5 units\/µl. One unit catalyses the incorporation of 10 nmol of dNTP into acid-insoluble material in 30 minutes at 70°C in 50 mM Tris-HCl (pH 9.0), 50 mM NaCl, 5 mM MgCl2, 200 µM dGTP, dATP, dTTP dCTP (a mix of un-labelled and [33P] dCTP), 10 µg of activated calf thymus DNA, and 0.1 mg\/ml BSA.\u003c\/p\u003e\u003cp\u003eStorage Buffer: 10 mM Tris-HCl (pH 7.5), 100 mM KCl, 0.1% Triton X-100, 0.1 mM EDTA, 1mM DTT, and 50% glycerol.\u003c\/p\u003e\u003cp\u003e10X Reaction Buffer:100mM Tris-HCl (pH 9.0), 500 mM KCl, 1% Triton X-100, and with or without 15mM MgCl2.\u003c\/p\u003e\u003cp\u003ePCR Activity: EconoTaq DNA Polymerase is tested in DNA amplification using a variety of templates and primers.\u003c\/p\u003e\u003cp\u003eActivity Determination: One unit of EconoTaq DNA Polymerase catalyses the incorporation of 10 nmoles of dNTP into acid-insoluble material in 30 minutes at 70 °C in 50 mM Tris-HCl (pH 9.0), 50 mM NaCl, 5 mM MgCl2, 200 µM dGTP, dATP, dTTP, dCTP (a mix of un-labelled and [33P]dCTP), 10 µg Activated Calf Thymus DNA, and 0.1 mg\/mL BSA.\u003c\/p\u003e\u003cp\u003eAbsence of Endonuclease or Nicking Activity: Incubation of 10 U of EconoTaq DNA Polymerase with 1 µg of supercoiled pBR322 DNA for 16 hours at 70 °C results in no detectable conversion to relaxed or linear forms detectable by agarose gel electrophoresis.\u003c\/p\u003e\u003cp\u003eAbsence of Exonuclease Activity: Incubation of 10 U of EconoTaq DNA Polymerase with 1 µg of HindIII-cut lambda DNA for 16 hours at 70 °C resulted in no smearing of bands on agarose gels.\u003c\/p\u003e\u003cp\u003ePurity: EconoTaq DNA Polymerase is \u0026gt;99% pure as determined by SDS PAGE. There is no detectable DNA contamination.\u003c\/p\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eEconoTaq DNA Polymerase is provided with a choice of 10X Reaction Buffer with Mg++ (“with Mg++”); or 10X Reaction Buffer without Mg++ and a separate tube of 25 mM MgCl2 (“separate Mg++”).\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-003_30031-1-EconoTaq-DNA-Polymerase-with-Mg++\"\u003e30031 1 EconoTaq DNA Polymerase with Mg++\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-003_30031-EconoTaq-DNA-Polymerase\"\u003e30031 EconoTaq DNA Polymerase\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-003_30032-3-EconoTaq-DNA-Polymerase-separate%20Mg++\"\u003e30032 3 EconoTaq DNA Polymerase separate Mg++\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-003_30032-EconoTaq-DNA-Polymerase\"\u003e30032 EconoTaq DNA Polymerase\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-003_SDS-30032-EconoTaq-DNA-Polymerasee\"\u003eSDS 30032 EconoTaq DNA Polymerasee\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_PCRAMP-003_EconoTaq\"\u003eEconoTaq\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_PCRAMP-003_EconoTaq-woMg\"\u003eEconoTaq woMg\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/pis_PCRAMP-003_Enzyme-Catalog-Full\"\u003eEnzyme Catalog Full\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/pis_PCRAMP-003_F201-US0617-EconoTaq\"\u003eF201 US0617 EconoTaq\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"5 U\/µL,  1,000 U, Buffer with Mg++","offer_id":45986429337679,"sku":"30031-1","price":784.0,"currency_code":"ILS","in_stock":true},{"title":"5 U\/µL,  1,000 U, Buffer without Mg++","offer_id":45986429370447,"sku":"30032-1","price":784.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/EconoTaq-DNA-Polymerase_bbe59935-f7f0-4abe-8999-132940b11d9c.jpg?v=1785956073"},{"product_id":"econotaq-plus-master-mix-pcramp-004","title":"EconoTaq PLUS Master Mix","description":"\u003cp\u003eMore effective than other Taq master mixes. EconoTaq PLUS and PLUS GREEN 2X Master Mixes contain a proprietary PCR enhancer, so they can successfully amplify templates that fail to amplify with other PCR master mixes (Figure 1). High density reaction buffer enables direct gel loading following PCR.\u003c\/p\u003e\u003cp\u003eExcellent results in routine PCR. EconoTaq PLUS GREEN \u0026amp; EconoTaq PLUS contain high purity, high activity EconoTaq DNA Polymerase for reliable amplification of templates up to 5 kb (Figure 1).\u003c\/p\u003e\u003cp\u003eAmplify challenging GC-rich templates. These 2X Master Mixes can be cycled up to 98 °C to amplify challenging GC-rich templates others cannot (Figure 2).\u003c\/p\u003e\u003cp\u003eConvenient tracking dyes in EconoTaq PLUS GREEN. On a 1% agarose gel, the blue dye migrates at the same rate as a 5 kb DNA fragment, and the yellow dye migrates at 75 bp. See Figure 3. If desired, the dyes are easily removed by standard DNA purification products or ethanol precipitation. For fluorescence or absorbance detection assays, EconoTaq PLUS 2X Master Mix offers the same great performance without the dyes.\u003c\/p\u003e\u003cp\u003eFlexible \u0026amp; easy to use. EconoTaq PLUS GREEN and EconoTaq PLUS are packaged in 50 reaction vials that can be stored in the refrigerator (+4 °C) for up to 3 months. They are stable for at least 10 freeze-thaw cycles.\u003c\/p\u003e\u003cp\u003eFigure 1. EconoTaq PLUS GREEN Master Mix vs. GoTaq® Green master mix (Competitor A) in PCR. PCR was performed according to the manufacturer’s recommendations using primers specific for the indicated genes. Panel A, 0.7 kb prokaryotic single-stranded DNA binding protein (genomic DNA); Panel B, 2.7 kb prokaryotic DNA polymerase A (genomic DNA); Panel C, 4.5 kb gene (plasmid DNA).\u003c\/p\u003e\u003cp\u003eFigure 2. GC-rich regions from human genomic DNA were PCR amplified using 98 °C denaturation.\u003c\/p\u003e\u003cp\u003eFigure 3. Easy visualization with EconoTaq PLUS GREEN. During electrophoresis, the green loading colour separates into a slow-moving blue dye and a fast-moving yellow dye.\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eOutstanding performance and value, perfect for routine PCR.\u003c\/li\u003e\n\u003cli\u003eCan be cycled up to 99 °C to amplify the most challenging GC-rich templates others cannot.\u003c\/li\u003e\n\u003cli\u003eReady-to-use PCR reaction master mixes, contain dNTPs and PCR Enhancer.\u003c\/li\u003e\n\u003cli\u003eNon-proofreading polymerase\u003c\/li\u003e\n\u003cli\u003eEconoTaq PLUS GREEN contains tracking dyes for gel electrophoresis\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cp\u003ePCR Activity: EconoTaq PLUS GREEN \u0026amp; EconoTaq PLUS 2X Master Mixes are tested in DNA amplification using a variety of templates and primers.\u003c\/p\u003e\u003cp\u003eActivity Determination: One unit of EconoTaq DNA Polymerase catalyses the incorporation of 10 nmoles of dNTP into acid-insoluble material in 30 minutes at 70°C in 50 mM Tris-HCl (pH 9.0), 50 mM NaCl, 5 mM MgCl2, 200 µM dGTP, dATP, dTTP, dCTP (a mix of un-labelled and [33P]dCTP), 10 µg Activated Calf Thymus DNA, and 0.1 mg\/ml BSA.\u003c\/p\u003e\u003cp\u003eAbsence of Endonuclease or Nicking Activity: Incubation of 10 U of EconoTaq DNA Polymerase with 1 µg of supercoiled pBR322 DNA for 16 hours at 70 °C results in no detectable conversion to relaxed or linear forms detectable by agarose gel electrophoresis.\u003c\/p\u003e\u003cp\u003eAbsence of Exonuclease Activity: Incubation of 10 U of EconoTaq DNA Polymerase with 1 µg of HindIII-cut lambda DNA for 16 hours at 70°C resulted in no smearing of bands on agarose gels.\u003c\/p\u003e\u003cp\u003ePurity: EconoTaq DNA Polymerase is \u0026gt;99% pure as determined by SDS PAGE. There is no detectable DNA contamination. The nucleotides in the Master Mix are certified free of nucleases and phosphatases.\u003c\/p\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eUse EconoTaq PLUS GREEN 2X Master Mix when the PCR products will be analysed by agarose gel electrophoresis and ethidium bromide staining. Use EconoTaq PLUS 2X Master Mix when the PCR products will be analysed by absorbance or fluorescence excitation, to avoid possible interference by the dyes. EconoTaq PLUS GREEN \u0026amp; EconoTaq PLUS prices are based on a 50 µL PCR reaction.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-004_30033-EconoTaq-PLUS-Green-2X-Master-Mix\"\u003e30033 EconoTaq PLUS Green 2X Master Mix\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-004_30035-EconoTaq-PLUS-2X-Master-Mix\"\u003e30035 EconoTaq PLUS 2X Master Mix\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-004_Danish-EU\"\u003eDanish EU\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-004_Dutch-EU\"\u003eDutch EU\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-004_English-EU\"\u003eEnglish EU\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-004_English-GB\"\u003eEnglish GB\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-004_English-US\"\u003eEnglish US\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-004_Finnish-EU\"\u003eFinnish EU\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-004_French-EU\"\u003eFrench EU\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-004_German-EU\"\u003eGerman EU\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-004_Italian-EU\"\u003eItalian EU\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-004_Norwegian-EU\"\u003eNorwegian EU\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-004_Portuguese-EU\"\u003ePortuguese EU\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-004_Spanish-EU\"\u003eSpanish EU\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-004_Swedish-EU\"\u003eSwedish EU\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_PCRAMP-004_EconoTaq-PLUS\"\u003eEconoTaq PLUS\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_PCRAMP-004_EconoTaq-PLUSGREEN\"\u003eEconoTaq PLUSGREEN\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/pis_PCRAMP-004_F201-US0617-EconoTaq\"\u003eF201 US0617 EconoTaq\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"500 x 50 µL Reactions","offer_id":45986429403215,"sku":"30033-1","price":1273.0,"currency_code":"ILS","in_stock":true},{"title":"500 x 50 µL Reactions (30035-1)","offer_id":45986429435983,"sku":"30035-1","price":1303.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/EconoTaq-PLUS-Master-Mix_395ddfaa-cf78-4ab4-bb9a-61da88a2ea6d.jpg?v=1785956073"},{"product_id":"rapidxfire-hot-start-taq-dna-polymerase-pcramp-005","title":"RapiDxFire Hot Start Taq DNA Polymerase","description":"\u003cp\u003eRapiDxFire Hot Start Taq DNA Polymerase is recombinant Taq DNA Polymerase from Thermus aquaticus bound to select blockers, which prevents unwanted, nonspecific amplification during reaction set up at temperatures less than 60 °C. Enzyme activity is restored after a short 15 second burst at 94 °C, enabling fast detection of low abundance target DNA. The Taq polymerase possesses 5′→3’ polymerase activity (amplicons up to 5 Kb) and dsDNA specific 5→3’ exonuclease activity, ideal for use with hydrolysis probes in qPCR reactions. The glycerol-free (GF), Triton®-free, and high- concentration formulation- is compatible with downstream lyophilisation methods and provides robust enzyme stability in liquid form (\u0026gt;28 days at 37 °C). These features provide flexible preparation and storage methods for qPCR master mixes (and detection assays) in addition to enabling benchtop reaction setups in warmer environments. Whether you are developing automated diagnostic tests or producing amplicons for downstream applications, RapiDxFire Hot Start Taq DNA Polymerase is your perfect choice for both demanding and routine PCR applications.\u003c\/p\u003e\u003cp\u003eApplications\u003c\/p\u003e\u003cp\u003eFood borne pathogen detection\u003cbr\u003eDiagnostic test development\u003cbr\u003eBiomarker discovery and monitoring\u003cbr\u003eGene expression analysis\u003cbr\u003eSNP genotyping\u003cbr\u003eCopy number variation (CNV) analysis\u003cbr\u003eCloning\u003cbr\u003eRapiDxFire Hot Start Taq DNA Polymerase is tightly controlled preventing non-specific amplification and enabling room temperature reaction set-up\u003c\/p\u003e\u003cp\u003eFigure 1. RapiDxFire Hot Start Taq DNA Polymerase performs better than three market-leading hot start Taq polymerases. The comparison was conducted using a E. coli gene-specific assay that produces a melt peak at ~89°C. PCR cycling conditions included a 90 minute pre-incubation at 35°C to induce non-specific amplification. Each polymerase was tested using 1.25 Units of Taq and supplier-recommended buffer in a 25 µL reaction using SYBR green detection. All three competitors exhibit non-specific amplification as seen by the second melt peak.\u003c\/p\u003e\u003cp\u003eShort activation time (15 sec) accelerates time to result\u003c\/p\u003e\u003cp\u003eFigure 2. Effect of RapiDxFire Hot Start Taq DNA Polymerase activation time on assay sensitivity. Target detection and PCR efficiency were compared when using a short 15-second hot start Taq activation time (green) versus a standard 2-minute activation time (red) at 94 °C prior to PCR cycling. RapiDxFire Hot Start Taq polymerase was tested using 0, 100, 1000, 10,000 copies of purified human DNA using a gene specific assay. Real-time detection of PCR products was performed using BHQplus™ probes and BioRad CFX C1000 Touch™ thermocycler.\u003c\/p\u003e\u003cp\u003eRapiDxFire Hot Start Taq DNA Pol is stable \u0026gt;28 days @ 37 ⁰C in glycerol-free storage buffer\u003c\/p\u003e\u003cp\u003eFigure 3. Stability of RapiDxFire Hot Start Taq polymerase in glycerol-free enzyme storage buffer. Hot Start Taq enzyme was subjected to -20 °C, 4 °C, 20-25 °C, and 37 °C for 28 days prior to performing real time PCR using an E. coli gene specific assay against template DNA and No Template Control (NTC) in a glycerol-free reaction buffer. Detection of PCR products (2.4 kb) was performed in real time using an intercalating dye and BioRad CFX C1000 Touch™.\u003c\/p\u003e\u003cp\u003eread more\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eFast activation time\u003c\/li\u003e\n\u003cli\u003eEnhanced enzyme stability in glycerol-free storage buffer (\u0026gt;28 days at 37°C)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cp\u003eConcentration: 5 U\/µL (Cat No. 30042-1, 30043-1) or 50 U\/µL (Cat. No. 30044-1).\u003c\/p\u003e\u003cp\u003eStorage: Store at -20 °C.\u003c\/p\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eRapiDxFire Hot Start Taq is provided with 10x Reaction Buffer and a separate tube of 25 mM MgCl2. RapiDxFire Hot Start Taq GF enzyme storage buffer is free of glycerol (Item ID. 30043-1 and 30044-1). All reagents are free of Triton X-100.\u003c\/p\u003e\n\u003ch2\u003eFAQs\u003c\/h2\u003e\n\u003cp\u003eFAQs\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-005_30042-1-RapiDxFire-Hot-Start-Taq-1000-Units-at-5-U\"\u003e30042 1 RapiDxFire Hot Start Taq 1000 Units at 5 U\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-005_30043-1-RapiDxFire-Hot-Start-Taq-GF-1000-Units-at-5-U\"\u003e30043 1 RapiDxFire Hot Start Taq GF 1000 Units at 5 U\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-005_30044-1-RapiDxFire-Hot-Start-Taq-GF-500-Units-at-50-U\"\u003e30044 1 RapiDxFire Hot Start Taq GF 500 Units at 50 U\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-005_SDS-30042-1-RapiDxFire-Hot-Start-Taq-1000-Units-at-5-U\"\u003eSDS 30042 1 RapiDxFire Hot Start Taq 1000 Units at 5 U\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-005_SDS-30043-1-RapiDxFire-Hot-Start-Taq-GF-1000-Units-at-5-U\"\u003eSDS 30043 1 RapiDxFire Hot Start Taq GF 1000 Units at 5 U\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-005_SDS-30044-1-RapiDxFire-Hot-Start-Taq-GF-500-Units-at-50-U\"\u003eSDS 30044 1 RapiDxFire Hot Start Taq GF 500 Units at 50 U\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_PCRAMP-005_manual-rapidxfire-hot-start-taq-dna-polymerase\"\u003emanual rapidxfire hot start taq dna polymerase\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"5 U\/µL, 1,000 U","offer_id":45986429501519,"sku":"30042-1","price":3146.0,"currency_code":"ILS","in_stock":true},{"title":"5 U\/uL,  5,000 U","offer_id":45986429534287,"sku":"30042-2","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"5 U\/µL, 1,000 U (30043-1)","offer_id":45986429567055,"sku":"30043-1","price":3558.0,"currency_code":"ILS","in_stock":true},{"title":"500 Units at 50 U\/µL","offer_id":45986429599823,"sku":"30044-1","price":1933.0,"currency_code":"ILS","in_stock":true},{"title":"1 mL at 5,000 U\/mL, 5,000 U","offer_id":45986429632591,"sku":"F835402-3","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/RapiDxFire-Hot-Start-Taq-DNA-Polymerase_9b6b411a-49e2-4968-93d9-81c2537f7d38.jpg?v=1785956076"},{"product_id":"rapidxfire-qpcr-5x-master-mix-with-ung-pcramp-105","title":"RapiDxFire™ qPCR 5X Master Mix with UNG","description":"\u003cp\u003eRapiDxFire qPCR 5X Master Mix + dUTP\/UNG (and ROX) is formulated for carry-over amplicon contamination control for use in automated, centralized pathogen testing workflows. This multiplex master mix contains RapiDxFIre Hot Start Taq DNA Polymerase, Uracil-N- Glycosylase (UNG), dNTPs with dUTP, optimized buffer components, and optional ROX reference dye.\u003c\/p\u003e\u003cp\u003eRapiDxFire qPCR 5X Master Mix GF has been optimised for use with all Biosearch Technologies’ probes (Dual-Labelled BHQ, BHQplus, BHQnova and BHQplex CoPrimers), which all contain Biosearch Technologies’ BHQ quenchers. BHQ dyes have been proven to dramatically reduce low-level background fluorescence due to highly efficient static quenching between reporter and quencher.\u003c\/p\u003e\u003cp\u003eThe RapiDxFire qPCR 5X Master Mix, manufactured in an ISO 13485-certified facility, is available in small development sizes to large scale production batches to assist you through the diagnostic test development process.\u003c\/p\u003e\u003cp\u003eRapiDxFire qPCR 5X Master Mix with UNG (and ROX) is intended for use as a general purpose reagent in molecular diagnostic assays that are based on qPCR target detection technologies by clinical laboratory professionals located in the United States. This product must be qualified and validated by clinical laboratory end users for suitability in the detection of any specific target using the procedures in this Manual as guidance. This product is classified as a general purpose in vitro diagnostic device reagent in the United States as defined in  21CFR 864.4010.\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e•  5X dye-free formulation  (with dUTP) offering flexible reaction setups and protocols\u003c\/li\u003e\n\u003cli\u003e•  Available with no or low ROX dye for broad instrument compatibility\u003c\/li\u003e\n\u003cli\u003e•  Designed for simultaneous detection of multiple targets in a single reaction (multiplexing)\u003c\/li\u003e\n\u003cli\u003e• Manufactured in an ISO 13485-certified facility\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eContains 1 mL (30051-1,30053-1) or 10 mL (30051-2, 30053-2) of RapiDxFire™ qPCR 5X Master Mix with or without ROX, respectively.\u003c\/p\u003e\u003cp\u003eFor Laboratory Use\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-105_RapiDxFire-qPCR-5X-Master-Mix-with-UN-and-ROX-US\"\u003eRapiDxFire qPCR 5X Master Mix with UN and ROX US\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-105_RapiDxFire-qPCR-5X-Master-Mix-with-UNG-US\"\u003eRapiDxFire qPCR 5X Master Mix with UNG US\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_PCRAMP-105_RapiDxFire-qPCR-5X-MM-with-dUTP-UNG-and-ROX\"\u003eRapiDxFire qPCR 5X MM with dUTP UNG and ROX\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"1 mL","offer_id":45986429730895,"sku":"30051-1","price":1977.0,"currency_code":"ILS","in_stock":true},{"title":"10 mL","offer_id":45986429763663,"sku":"30051-2","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"1 mL (30053-1)","offer_id":45986429796431,"sku":"30053-1","price":1977.0,"currency_code":"ILS","in_stock":true},{"title":"10 mL (30053-2)","offer_id":45986429829199,"sku":"30053-2","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/RapiDxFire_25E2_2584_25A2-qPCR-5X-Master-Mix-with-UNG_a6cc3262-0509-4e7a-853b-9274ace952cc.jpg?v=1785956080"},{"product_id":"rapidxfire-qpcr-5x-master-mix-gf-pcramp-012","title":"RapiDxFire qPCR 5X Master Mix GF","description":"\u003cp\u003eFormulated with diagnostic kit developers in mind, RapiDxFire qPCR 5X Master Mix glycerol free (GF) can be combined with gene-specific primers and hydrolysis probes for immediate use in high-throughput laboratory developed test (LDT) workflows or further processed for lyophilisation for use in point of care (POC) devices. Provided at a 5X concentration without passive reference dye, this flexible master mix allows more room for sample and up to 5 target detection at one time. The RapiDxFire qPCR 5X Master Mix, manufactured in an ISO 13485-certified facility, is available in small development sizes to large scale production batches to assist you through the diagnostic test development process.\u003c\/p\u003e\u003cp\u003eRapiDxFire qPCR 5X Master Mix GF has been optimised for use with all Biosearch Technologies’ probes (Dual-Labelled BHQ, BHQplus, BHQnova and BHQplex CoPrimers), which all contain Biosearch Technologies’ proprietary BHQ quenchers. BHQ dyes have been proven to dramatically reduce low-level background fluorescence due to highly efficient static quenching between reporter and quencher. BHQ dyes are compatible with reporter fluorescent dyes that span the visible spectrum, allowing for broad flexibility in fluorophore selection. RapiDxFire qPCR 5X Master Mix GF can also be used with any probe-based assay. In addition, the Biosearch Technologies RealTimeDesign software is available online to facilitate the design of qPCR assays. Research use only, not for use in diagnostic procedures.\u003c\/p\u003e\u003cp\u003eApplications\u003c\/p\u003e\u003cp\u003ePathogen detection\u003cbr\u003eFood safety testing\u003cbr\u003eBiomarker discovery and monitoring\u003cbr\u003eGene expression analysis in two-step RT-qPCR\u003c\/p\u003e\u003cp\u003eSensitive detection down to 10 DNA copies in singleplex and 5-plex qPCR assays\u003c\/p\u003e\u003cp\u003eFigure 1. Singleplex (A) and multiplex (B) amplification plots with RapiDxFire qPCR Master Mix using 10-fold dilution series. cDNA was synthesized from MS2 phage RNA and amplified using five different assays with BHQ probes. The data displayed here is the target reported with Quasar 705. Template DNA (5 µL) was added to RapiDxFire qPCR Master Mix reaction mix (15 µL) and performed under standard cycling conditions. Sensitive and reproducible detection of 10 to 1,000,000 copies of cDNA was achieved. For both singleplex and multiplex reactions, standard curves for all targets generated a PCR efficiency of 90-110% and an R2 value of 0.995 to 0.999.\u003c\/p\u003e\u003cp\u003eAutomation-friendly 48 hour benchtop stability of fully assembled reactions\u003c\/p\u003e\u003cp\u003eFigure 2. Amplification plots of a 4-plex RapiDxFire qPCR fully assembled reaction stored at room temperature over time prior to PCR cycling. We tested 1 hour, 24 hour or 48 hour pre-thermal cycling incubation times with reactions containing RapiDxFire qPCR Master Mix, BHQ assays, and DNA template. A multiplex assay targeting four individual mouse genomic DNA targets using BHQ probes and primers at a standard concentration of 200 nM and 900 nM, respectively, reporting with FAM and Biosearch Technologies proprietary dyes CAL Fluor Orange 560, CAL Fluor Red 610 and Quasar 670, was amplified using standard cycling conditions. A completely assembled reaction can be left on the bench for up to 48 hours without affecting the range of detection down to 10 copies.\u003c\/p\u003e\u003cp\u003eReliable lot to lot uniformity\u003c\/p\u003e\u003cp\u003eFigure 3. The Cq mean and standard deviation of three independent lots of RapiDxFire qPCR Master Mix. Three lots of BHQ Probe Master Mix were each tested with BHQ probes addressing four individual targets in a multiplex reaction. The cumulative deviation was \u0026lt; Cq 0.5 between each replicate (3 x 10 replicates) at the 10 copy level across three different lots.\u003c\/p\u003e\u003cp\u003eRapiDxFire qPCR Master Mix outperforms other master mixes in accurately quantifying pathogens during challenging multiplex reactions.\u003c\/p\u003e\u003cp\u003eSample\tOrganism (1,000,000 copies\/rxn)\tOrganism (100 copies\/rxn)\u003cbr\u003e1\tS. typhi\tP. aeruginosa \u0026amp; S. liquifacens \u0026amp; E. coli\u003cbr\u003e2\tE. coli\tP. aeruginosa \u0026amp; S. liquifacens \u0026amp; S. typhi\u003cbr\u003e3\tP. aeruginosa\tS. liquifacens \u0026amp; S. typhi \u0026amp; P. aeruginosa\u003cbr\u003e4\tS. liquifacens\tS. typhi \u0026amp; P. aeruginosa \u0026amp; S.typhi\u003cbr\u003e5\tS. typhi \u0026amp; E. coli\tP. aeruginosa \u0026amp; S. liquifacens\u003cbr\u003e6\tP. aeruginosa \u0026amp; S. liquifacens\tS. typhi \u0026amp; E. coli\u003cbr\u003e7\tP. aeruginosa \u0026amp; S. liquifacens \u0026amp; E. coli\tS. typhi\u003c\/p\u003e\u003cp\u003eFigure 4. Comparison of RapiDxFire qPCR Master Mix to other competitor master mixes in quantifying Salmonella typhi among varying levels of other pathogenic bacteria. A series of multiplex experiments were performed to detect S. typhi in the background of increasingly abundant background of genomic DNA from pathogenic organisms, Escherichia coli, Pseudomonas aeruginosa, and Serratia liquefaciens. BHQ probe (200 nM) and primer (500 nM) concentrations were fixed for each experiment. Thermal cycling conditions were as stated by the manufacturer. Each reaction was run in 20 µL volumes with 5 µL of template to a final concentration of either 1,000,000 or 100 target copies (ratios indicated in Table 2). RapiDxFire qPCR Master Mix most accurately quantified S. typhi in all samples, and was the only master mix of appropriate sensitivity to reliably detect the presence of S. typhi in the most challenging sample\u003c\/p\u003e\u003cp\u003e.\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e5X dye-free formulation offering flexible reaction setups and protocols\u003c\/li\u003e\n\u003cli\u003eSensitive detection\u003c\/li\u003e\n\u003cli\u003eWide dynamic range for multiplexing\u003c\/li\u003e\n\u003cli\u003e48-hour reaction benchtop stability ideal for automated workflows\u003c\/li\u003e\n\u003cli\u003eGlycerol-free, Triton-free, high concentration, and bulk formulations for adaptable test development and lyophilisation options.\u003c\/li\u003e\n\u003cli\u003eManufactured in an ISO 13485-certified facility demonstrating batch to batch reproducibility\u003c\/li\u003e\n\u003cli\u003eComponent of the RapiDxFire 1-step RT-qPCR system for effective detection of viral RNA pathogens\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cp\u003eConcentration: 5X Master Mix\u003c\/p\u003e\u003cp\u003eStorage: Store at -20 °C.\u003c\/p\u003e\u003cp\u003eMaster Mix buffer: Glycerol-free and Triton™ X-100-free buffer\u003c\/p\u003e\u003cp\u003eContaminating activity assays: RapiDxFire qPCR 5X Master Mix GF is free of detectable contaminating DNA exonuclease, and DNA endonuclease.\u003c\/p\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eRapiDxFire qPCR 5X Master Mix GF is also a component of the RapiDxFire 1-step RT-qPCR System.\u003c\/p\u003e\u003cp\u003eFor Laboratory Use.\u003c\/p\u003e\n\u003ch2\u003eResources\u003c\/h2\u003e\n\u003cp\u003eApplication notes\u003c\/p\u003e\u003cp\u003eRapiDxFire qPCR5 X MasterMix GF\u003c\/p\u003e\u003cp\u003erapidxfire 1 step rt qpcr sars cov 2 detection\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-012_30050-1-RapiDxFire-qPCR-5X-Master-Mix-Glycerol-Free-1-mL\"\u003e30050 1 RapiDxFire qPCR 5X Master Mix Glycerol Free 1 mL\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-012_30050-100ML-RapiDxFire-qPCR-5X-Master-Mix-Glycerol-Free-100-mL\"\u003e30050 100ML RapiDxFire qPCR 5X Master Mix Glycerol Free 100 mL\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-012_30050-2-RapiDxFire-qPCR-5X-Master-Mix-Glycerol-Free-10-mL\"\u003e30050 2 RapiDxFire qPCR 5X Master Mix Glycerol Free 10 mL\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_PCRAMP-012_quick-guide-rapidxfire-qpcr-5x-master-mix-gf\"\u003equick guide rapidxfire qpcr 5x master mix gf\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_PCRAMP-012_user-guide-rapidxfire-qpcr-5x-master-mix-gf\"\u003euser guide rapidxfire qpcr 5x master mix gf\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/app-note_PCRAMP-012_RapiDxFire-qPCR5-X-MasterMix-GF\"\u003eRapiDxFire qPCR5 X MasterMix GF\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/app-note_PCRAMP-012_rapidxfire-1-step-rt-qpcr-sars-cov-2-detection\"\u003erapidxfire 1 step rt qpcr sars cov 2 detection\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"1 mL","offer_id":45986429894735,"sku":"30050-1","price":2020.0,"currency_code":"ILS","in_stock":true},{"title":"100 mL","offer_id":45986429927503,"sku":"30050-100ML","price":157.0,"currency_code":"ILS","in_stock":true},{"title":"10 mL","offer_id":45986429960271,"sku":"30050-2","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/RapiDxFire-qPCR-5X-Master-Mix-GF.jpg?v=1785956080"},{"product_id":"rapidxfire-lyo-flex-1-step-rt-qpcr-5x-master-mix-pcramp-021","title":"RapiDxFire Lyo-Flex 1-Step RT-qPCR 5X Master Mix","description":"\u003cp\u003eRapiDxFire™ Lyo-Flex 1-Step RT-qPCR 5X Master Mix is a convenient, single-tube master mix that provides sensitive and reproducible detection of bacterial and viral pathogens. Optimised for multiplexing with gene-specific primers and BHQ probes, this temperature-stable, glycerol-free, mix is ideal for molecular diagnostic kit manufacturers developing traditional tests for high-throughput, automated workstreams or lyophilised tests for POC devices.\u003c\/p\u003e\u003cp\u003eProvided at a 5X concentration without passive reference dye, this robust master mix includes a heat-activated thermostable reverse transcriptase, Taq DNA polymerase, and dNTPs (including dUTP) in a single, Triton-free, glycerol-free buffered solution. Compatible with added Uracil N-Glycolsylase (UNG) to control carryover contamination and\/or an RNase inhibitor for template preservation, RapiDxFire Lyo-Flex 1-Step RT-qPCR 5X Master Mix is a versatile component for diagnostic test development. RapiDxFire Lyo-Flex 1-Step RT-qPCR 5X Master Mix is manufactured in an ISO 13485-certified facility and is available in suitable test development sizes to large scale production lots.\u003c\/p\u003e\u003cp\u003eRapiDxFire Lyo-Flex 1-Step RT-qPCR 5X Master Mix is a General Purpose Reagent (GPR), labelled For Laboratory Use (FLU). The FLU label statement identifies an in vitro diagnostic medical device in the United States. Outside the United States the FLU statement is equivalent to Research Use Only.\u003c\/p\u003e\u003cp\u003eSensitive detection of RNA and DNA targets\u003c\/p\u003e\u003cp\u003eFigure 1. Broad dynamic range for both RNA and DNA targets. (a) SARS-CoV-2 RNA control (Twist) 10-fold serially diluted from 1x106-1x100 copies per reaction, on which the UltraDx SARS-CoV-2 N1\/N2\/RnP assay (Biosearch Technologies) was performed (N1\/N2:FAM shown) (20 µL total reaction volume). (b) genomic mouse DNA 10-fold serially diluted from 300,000 to 3 copies per reaction on which the Isg20 assay was performed (N1\/N2:FAM shown) (20 µL total reaction volume). PCR efficiencies (90%-110%) and R2 values \u0026gt;0.98 for (a) SARS-CoV-2 RNA and (b) genomic mouse DNA assays met PCR criteria.\u003c\/p\u003e\u003cp\u003eEnhanced reaction stability at room temperature\u003c\/p\u003e\u003cp\u003eFigure 2. Fully assembled reaction mix at room temperature gives stable and sensitive Cq values for all targets for up to 8 hours. Genomic MS2 RNA (100 copies per reaction, 20 µL total reaction volume) tested in triplex with each MS2-region specific BHQ™ Probe assigned a unique fluorophore ((a) R6D1:FAM; (b) R3D3:CAL Fluor Orange 560 (c) R8D1:Quasar 705). RapiDxFire Lyo-Flex 1-Step RT-qPCR 5X Master Mix was tested against Competitor A and Competitor B, according to manufacturer’s recommendations. The lower the Cq value, the more sensitive the detection. A Cq value ≥50 is indicative of no amplification.\u003c\/p\u003e\u003cp\u003eLyo-compatible formulation\u003c\/p\u003e\u003cp\u003eFigure 3. Consistent performance of RapiDxFire Lyo-Flex 1-Step RT-qPCR 5X Master Mix post-lyophilisation by Vendor A, supplemented with 4 mM MgCl2. Lyophilised spheres were tested against genomic MS2 RNA 10-fold serially diluted from 1x107-1x102 copies per reaction (20 µL total reaction volume), with each MS2-region specific BHQ Probe assigned a unique fluorophore (R6D1:FAM; R3D3:CAL Fluor Orange 560; R8D1:Quasar 705). (a) pre-lyophilisation and (b) post-lyophilisation amplification curves, tested with the R6D1:FAM assay, and standard curves for all targets, with PCR efficiencies (90%-110%) and R2 values \u0026gt;0.98, meeting PCR criteria.\u003c\/p\u003e\u003cp\u003eLyobeads remain stable at room temperature over time\u003c\/p\u003e\u003cp\u003eFigure 4. Lyobead stability demonstrated after 0 and 4 month storage at room temperature (25 °C). Lyobeads (manufactured by Vendor B) were prepared with RapiDxFire Lyo-Flex 1-Step RT-qPCR 5X Master Mix and tested against genomic MS2 RNA 10-fold serially diluted from 1x107-1x102 copies per reaction (20 µL total reaction volume), with each MS2-region specific BHQ Probe assigned a unique fluorophore (R6D1:FAM; R3D3:CAL Fluor Orange 560; R8D1:Quasar 705). (a) 0 month and (b) 4-month time point amplification curves, tested with the R6D1:FAM assay and standard curves for all targets, with PCR efficiencies (90%-110%) and R2 values \u0026gt;0.98, meeting PCR criteria.\u003c\/p\u003e\u003cp\u003eLimitations:\u003cbr\u003eResults are a property of both the assay and template type\/concentration in the sample, as well as the formulation of the master mix itself. As such, individual results may vary.\u003c\/p\u003e\u003cp\u003eread more\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eHigh sensitivity to detect low copy RNA and DNA targets in single- and multiplex reactions\u003c\/li\u003e\n\u003cli\u003eSingle tube, room temperature-stable formulation streamlines automated workflows\u003c\/li\u003e\n\u003cli\u003eGlycerol-free components for flexible lyophilisation options\u003c\/li\u003e\n\u003cli\u003e5X concentration allows increased sample addition in liquid reactions or smaller lyobead formation during manufacturing\u003c\/li\u003e\n\u003cli\u003eGeneral purpose reagent manufactured in an ISO 13485-certified facility\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eResources\u003c\/h2\u003e\n\u003cp\u003eApplication notes\u003c\/p\u003e\u003cp\u003etechnical note RapiDxFire Lyo Flex 1 Step RT qPCR 5X Master Mix\u003c\/p\u003e\u003cp\u003etechnical note RapiDxFire Lyo Flex 1 Step RT qPCR 5X Master Mix\u003c\/p\u003e\n\u003ch2\u003eFAQs\u003c\/h2\u003e\n\u003cp\u003eFAQs\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-021_rapidxfire-lyo-flex-1-step-rt-qpcr-5x-master-mix-2021-eu\"\u003erapidxfire lyo flex 1 step rt qpcr 5x master mix 2021 eu\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-021_rapidxfire-lyo-flex-1-step-rt-qpcr-5x-master-mix-2021-eu-de\"\u003erapidxfire lyo flex 1 step rt qpcr 5x master mix 2021 eu de\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-021_rapidxfire-lyo-flex-1-step-rt-qpcr-5x-master-mix-2021-us\"\u003erapidxfire lyo flex 1 step rt qpcr 5x master mix 2021 us\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/protocol_PCRAMP-021_rapidxfire-lyo-flex-1-step-rt-qpcr-5x-master-mix\"\u003erapidxfire lyo flex 1 step rt qpcr 5x master mix\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_PCRAMP-021_rapidxfire-lyo-flex-1-step-rt-qpcr-5x-master-mix\"\u003erapidxfire lyo flex 1 step rt qpcr 5x master mix\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/data-sheet_PCRAMP-021_rapidxfire-lyo-flex-1-step-rt-qpcr-5x-master-mix\"\u003erapidxfire lyo flex 1 step rt qpcr 5x master mix\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/appnote_PCRAMP-021_technical-note-RapiDxFire-Lyo-Flex-1-Step-RT-qPCR-5X-Master-Mix\"\u003etechnical note RapiDxFire Lyo Flex 1 Step RT qPCR 5X Master Mix\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/app-note_PCRAMP-021_technical-note-RapiDxFire-Lyo-Flex-1-Step-RT-qPCR-5X-Master-Mix\"\u003etechnical note RapiDxFire Lyo Flex 1 Step RT qPCR 5X Master Mix\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"1mL","offer_id":45986430189647,"sku":"30060-1","price":3440.0,"currency_code":"ILS","in_stock":true},{"title":"10mL","offer_id":45986430222415,"sku":"30060-2","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"100 mL","offer_id":45986430255183,"sku":"30060-3","price":235.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/RapiDxFire-Lyo-Flex-1-Step-RT-qPCR-5X-Master-Mix_9bd13028-8cd8-410c-acca-9044e8e777c8.jpg?v=1785956084"},{"product_id":"dna-polymerase-i-large-klenow-fragment-30098-1","title":"DNA Polymerase I Large (Klenow) Fragment","description":"\u003cp\u003eDNA Polymerase I, Large (Klenow) Fragment is a DNA-dependent polymerase that lacks the 5′→3′ exonuclease activities of E. coli DNA Polymerase I from which it is derived. The 3′→5′ remains intact to remove 3’ overhangs, and along with the polymerase activity to fill in 5’ overhangs and create blunt end DNA fragments.  The optimal incubation temperature of the enzyme is 25 °C and uses 10X DNA Polymerase Buffer A. Heat inactivation of the enzyme is 75 °C for 20 minutes.\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eForms blunt ends by removing 3’ overhangs or filling in 5’ overhangs\u003c\/li\u003e\n\u003cli\u003eUsed in second-strand cDNA synthesis\u003c\/li\u003e\n\u003cli\u003eGenerates DNA probes using random primers\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eDNA Polymerase I Large (Klenow) Fragment includes Klenow DNA Polymerase at 5 U\/µL and 10X DNA Polymerase Buffer A\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_30098-1_10X-DNA-Polymerase-Buffer-A\"\u003e10X DNA Polymerase Buffer A\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_30098-1_DNA-Polymerase-I-Large-(Klenow)-Fragment\"\u003eDNA Polymerase I Large (Klenow) Fragment\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_30098-1_DNA-Polymerase-I-Large-(Klenow)-Fragment\"\u003eDNA Polymerase I Large (Klenow) Fragment\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"Default Title","offer_id":45986430320719,"sku":"30098-1","price":1554.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/DNA-Polymerase-I-Large-_2528Klenow_2529-Fragment_54d65d55-83f7-43f2-b901-c3f38638ae12.jpg?v=1785956086"},{"product_id":"cod-ung-pcramp-101","title":"Cod UNG","description":"\u003cp\u003eCod Uracil N-glycosylase (UNG) is a thermolabile enzyme from Atlantic cod (Gadus morhua) that catalyzes the removal of uracil bases from single-stranded or double-stranded DNA molecules, leaving an abasic site in the strand. The enzyme does not react with RNA.\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eSupplied at 1 U\/µL\u003c\/li\u003e\n\u003cli\u003eOptimal temperature range is 20-35 °C enabling 1-step RT-qPCR\u003c\/li\u003e\n\u003cli\u003eRapidly inactivated at 55 °C thus ensuring amplicon integrity during long-term storage\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eContains either 1 x 1 mL tube (300CUNG-1) or 5 x 1 mL tubes (300CUNG-2) of Cod UNG, 1U\/µL.\u003c\/p\u003e\u003cp\u003eResearch Use Only\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-101_COD-UNG-CA\"\u003eCOD UNG CA\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-101_COD-UNG-CAFR\"\u003eCOD UNG CAFR\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-101_COD-UNG-DE\"\u003eCOD UNG DE\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-101_COD-UNG-DEEN\"\u003eCOD UNG DEEN\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-101_COD-UNG-DK\"\u003eCOD UNG DK\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-101_COD-UNG-ES\"\u003eCOD UNG ES\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-101_COD-UNG-FI\"\u003eCOD UNG FI\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-101_COD-UNG-FR\"\u003eCOD UNG FR\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-101_COD-UNG-GB\"\u003eCOD UNG GB\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-101_COD-UNG-IT\"\u003eCOD UNG IT\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-101_COD-UNG-NL\"\u003eCOD UNG NL\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-101_COD-UNG-NO\"\u003eCOD UNG NO\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-101_COD-UNG-PT\"\u003eCOD UNG PT\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-101_COD-UNG-SE\"\u003eCOD UNG SE\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-101_COD-UNG-US\"\u003eCOD UNG US\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_PCRAMP-101_Cod-UNG\"\u003eCod UNG\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"1U\/uL, 1000 units","offer_id":45986430386255,"sku":"300CUNG-1","price":1467.0,"currency_code":"ILS","in_stock":true},{"title":"1U\/uL, 5000 units","offer_id":45986430419023,"sku":"300CUNG-2","price":6680.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/Cod-UNG_69581b28-3538-43ae-b25f-0a7297056eea.jpg?v=1785956090"},{"product_id":"2x-hotstart-qpcr-mastermix-pcramp-100","title":"2X HotStart qPCR MasterMix","description":"\u003cp\u003eAn inhibitor tolerant 2X master mix for endpoint or qPCR applications\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eIdeal for hydrolysis probe-based detection\u003c\/li\u003e\n\u003cli\u003eContains chemically modified Hot Start Taq DNA     Polymerase, which is activated at 95 °C for 2 minutes\u003c\/li\u003e\n\u003cli\u003eFormatted for 20 µL reaction size\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eContains either 2 x 1 mL tubes (300ITMM-1) or 6 x 1 mL tubes (300ITMM-2) of 2X HotStart qPCR MasterMIx\u003c\/p\u003e\u003cp\u003eResearch Use Only\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-100_2X-HotStart-qPCR-MasterMix-CA\"\u003e2X HotStart qPCR MasterMix CA\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-100_2X-HotStart-qPCR-MasterMix-CAFR\"\u003e2X HotStart qPCR MasterMix CAFR\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-100_2X-HotStart-qPCR-MasterMix-DE\"\u003e2X HotStart qPCR MasterMix DE\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-100_2X-HotStart-qPCR-MasterMix-DEEN\"\u003e2X HotStart qPCR MasterMix DEEN\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-100_2X-HotStart-qPCR-MasterMix-DK\"\u003e2X HotStart qPCR MasterMix DK\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-100_2X-HotStart-qPCR-MasterMix-ES\"\u003e2X HotStart qPCR MasterMix ES\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-100_2X-HotStart-qPCR-MasterMix-FI\"\u003e2X HotStart qPCR MasterMix FI\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-100_2X-HotStart-qPCR-MasterMix-FR\"\u003e2X HotStart qPCR MasterMix FR\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-100_2X-HotStart-qPCR-MasterMix-GB\"\u003e2X HotStart qPCR MasterMix GB\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-100_2X-HotStart-qPCR-MasterMix-IT\"\u003e2X HotStart qPCR MasterMix IT\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-100_2X-HotStart-qPCR-MasterMix-NL\"\u003e2X HotStart qPCR MasterMix NL\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-100_2X-HotStart-qPCR-MasterMix-NO\"\u003e2X HotStart qPCR MasterMix NO\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-100_2X-HotStart-qPCR-MasterMix-PT\"\u003e2X HotStart qPCR MasterMix PT\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-100_2X-HotStart-qPCR-MasterMix-SE\"\u003e2X HotStart qPCR MasterMix SE\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-100_2X-HotStart-qPCR-MasterMix-US\"\u003e2X HotStart qPCR MasterMix US\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_PCRAMP-100_2X-HotStart-qPCR-MasterMix\"\u003e2X HotStart qPCR MasterMix\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"200 rxns","offer_id":45986430484559,"sku":"300ITMM-1","price":891.0,"currency_code":"ILS","in_stock":true},{"title":"600 rxns","offer_id":45986430517327,"sku":"300ITMM-2","price":2523.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/2X-HotStart-qPCR-MasterMix_3a3497cb-dd2d-4d87-8afd-2cf985273f5f.jpg?v=1785956093"},{"product_id":"salt-tolerant-nuclease-300stn-1","title":"Salt Tolerant Nuclease","description":"\u003cp\u003eSalt tolerant nuclease is a recombinant enzyme from a psychrophilic marine bacterium, often used to reduce host-cell DNA contamination during production of viral particles (e.g. AAV) or recombinant proteins.\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eEnzyme is supplied at 25 U\/µL\u003c\/li\u003e\n\u003cli\u003eDegrades both single-stranded and double-stranded nucleic acids.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eContains 1 mL of salt tolerant nuclease, 25 U\/µL.\u003c\/p\u003e\u003cp\u003eResearch Use Only\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_300STN-1_Salt-Tolerant-Nuclease-CA\"\u003eSalt Tolerant Nuclease CA\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_300STN-1_Salt-Tolerant-Nuclease-CAFR\"\u003eSalt Tolerant Nuclease CAFR\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_300STN-1_Salt-Tolerant-Nuclease-DE\"\u003eSalt Tolerant Nuclease DE\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_300STN-1_Salt-Tolerant-Nuclease-DEEN\"\u003eSalt Tolerant Nuclease DEEN\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_300STN-1_Salt-Tolerant-Nuclease-DK\"\u003eSalt Tolerant Nuclease DK\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_300STN-1_Salt-Tolerant-Nuclease-ES\"\u003eSalt Tolerant Nuclease ES\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_300STN-1_Salt-Tolerant-Nuclease-FI\"\u003eSalt Tolerant Nuclease FI\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_300STN-1_Salt-Tolerant-Nuclease-FR\"\u003eSalt Tolerant Nuclease FR\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_300STN-1_Salt-Tolerant-Nuclease-GB\"\u003eSalt Tolerant Nuclease GB\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_300STN-1_Salt-Tolerant-Nuclease-IT\"\u003eSalt Tolerant Nuclease IT\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_300STN-1_Salt-Tolerant-Nuclease-NL\"\u003eSalt Tolerant Nuclease NL\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_300STN-1_Salt-Tolerant-Nuclease-NO\"\u003eSalt Tolerant Nuclease NO\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_300STN-1_Salt-Tolerant-Nuclease-PT\"\u003eSalt Tolerant Nuclease PT\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_300STN-1_Salt-Tolerant-Nuclease-SE\"\u003eSalt Tolerant Nuclease SE\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_300STN-1_Salt-Tolerant-Nuclease-US\"\u003eSalt Tolerant Nuclease US\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_300STN-1_Salt-tolerant-nuclease\"\u003eSalt tolerant nuclease\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"Default Title","offer_id":45986430615631,"sku":"300STN-1","price":2147.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/Salt-Tolerant-Nuclease_f534dd8a-a606-4ee9-b9f4-366a32023657.jpg?v=1785956095"},{"product_id":"t7-exonuclease-kit-pcramp-103","title":"T7 Exonuclease Kit","description":"\u003cp\u003eVersatile DNase used in NGS library preparation, Sanger sequencing, site-directed mutagenesis and nicked site extension applications.\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eEnzyme is supplied at 10,000 U\/mL and includes 10X Reaction Buffer\u003c\/li\u003e\n\u003cli\u003eCleaves linear or nicked double-stranded DNA in the 5’\u0026gt;3’ direction, releasing mononucleotides.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eContains either 1 x 0.25 mL vial (300T7EXO-1) or 2 x 0.25 mL vials (300T7EXO-2) of T7 Exonuclease. A tube of Exonuclease Reaction Buffer,10X is also included.\u003c\/p\u003e\u003cp\u003eResearch Use Only\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-103_T7-Exonuclease-Kit-CAFR\"\u003eT7 Exonuclease Kit CAFR\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-103_T7-Exonuclease-Kit-DE\"\u003eT7 Exonuclease Kit DE\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-103_T7-Exonuclease-Kit-DEEN\"\u003eT7 Exonuclease Kit DEEN\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-103_T7-Exonuclease-Kit-DK\"\u003eT7 Exonuclease Kit DK\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-103_T7-Exonuclease-Kit-ES\"\u003eT7 Exonuclease Kit ES\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-103_T7-Exonuclease-Kit-FI\"\u003eT7 Exonuclease Kit FI\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-103_T7-Exonuclease-Kit-FR\"\u003eT7 Exonuclease Kit FR\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-103_T7-Exonuclease-Kit-GB\"\u003eT7 Exonuclease Kit GB\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-103_T7-Exonuclease-Kit-NL\"\u003eT7 Exonuclease Kit NL\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-103_T7-Exonuclease-Kit-NO\"\u003eT7 Exonuclease Kit NO\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-103_T7-Exonuclease-Kit-PT\"\u003eT7 Exonuclease Kit PT\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-103_T7-Exonuclease-Kit-SE\"\u003eT7 Exonuclease Kit SE\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-103_T7-Exonuclease-Kit-US\"\u003eT7 Exonuclease Kit US\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_PCRAMP-103_T7-Exonuclease-Kit\"\u003eT7 Exonuclease Kit\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"2500 units","offer_id":45986430713935,"sku":"300T7EXO-1","price":650.0,"currency_code":"ILS","in_stock":true},{"title":"5000 units","offer_id":45986430746703,"sku":"300T7EXO-2","price":1216.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/T7-Exonuclease-Kit_4880c9b3-2344-4519-b12f-a424b17044f9.jpg?v=1785956098"},{"product_id":"thermostable-pyrophosphatase-pcramp-102","title":"Thermostable Pyrophosphatase","description":"\u003cp\u003eThermostable pyrophosphatase is a recombinant, heat-tolerant (\u0026gt;95°C) acidic pyrophosphatase from a thermophilic archaebacterium. This enzyme selectively hydrolyzes inorganic pyrophosphate into two orthophosphate ions under standard buffered conditions (pH 6-9) but will not degrade other molecules containing phosphodiester bonds (e.g. nucleotide triphosphates). Inorganic pyrophosphatases can be added to DNA synthesis and amplification reactions to improve reaction kinetics.\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eSupplied at 2 U\/µL\u003c\/li\u003e\n\u003cli\u003eStable at temperatures \u0026gt; 95 °C\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eContains either 1 x 0.25 mL vial (300TAPP-1) or 4 x 0.25 mL vials (300TAPP-2) of Thermostable Pyrophosphatase, 2 U\/µL.\u003c\/p\u003e\u003cp\u003eResearch Use Only\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-102_Thermostable-Pyrophosphatase-CA\"\u003eThermostable Pyrophosphatase CA\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-102_Thermostable-Pyrophosphatase-CAFR\"\u003eThermostable Pyrophosphatase CAFR\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-102_Thermostable-Pyrophosphatase-DE\"\u003eThermostable Pyrophosphatase DE\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-102_Thermostable-Pyrophosphatase-DEEN\"\u003eThermostable Pyrophosphatase DEEN\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-102_Thermostable-Pyrophosphatase-DK\"\u003eThermostable Pyrophosphatase DK\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-102_Thermostable-Pyrophosphatase-ES\"\u003eThermostable Pyrophosphatase ES\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-102_Thermostable-Pyrophosphatase-FI\"\u003eThermostable Pyrophosphatase FI\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-102_Thermostable-Pyrophosphatase-FR\"\u003eThermostable Pyrophosphatase FR\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-102_Thermostable-Pyrophosphatase-GB\"\u003eThermostable Pyrophosphatase GB\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-102_Thermostable-Pyrophosphatase-IT\"\u003eThermostable Pyrophosphatase IT\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-102_Thermostable-Pyrophosphatase-NL\"\u003eThermostable Pyrophosphatase NL\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-102_Thermostable-Pyrophosphatase-NO\"\u003eThermostable Pyrophosphatase NO\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-102_Thermostable-Pyrophosphatase-SE\"\u003eThermostable Pyrophosphatase SE\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-102_Thermostable-Pyrophosphatase-US\"\u003eThermostable Pyrophosphatase US\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_PCRAMP-102_Thermostable-Pyrophosphatase\"\u003eThermostable Pyrophosphatase\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"2U\/uL, 500 units","offer_id":45986430976079,"sku":"300TAPP-1","price":717.0,"currency_code":"ILS","in_stock":true},{"title":"2U\/uL, 2000 units","offer_id":45986431008847,"sku":"300TAPP-2","price":2546.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/Thermostable-Pyrophosphatase_423c0baf-86c2-4a0a-b418-c8f062481065.jpg?v=1785956104"},{"product_id":"nxgen-phi29-dna-polymerase-pcramp-011","title":"NxGen phi29 DNA Polymerase","description":"\u003cp\u003eNxGen Phi29 DNA Polymerase is a highly processive, high-fidelity DNA polymerase that is derived from bacteriophage phi29.\u003c\/p\u003e\u003cp\u003eIt is an isothermal DNA polymerase with high strand displacement activity and a 3′→ 5′ proofreading exonuclease function that can amplify DNA with high specificity and accuracy, even from limited or degraded samples.\u003c\/p\u003e\u003cp\u003eIdeal applications:\u003c\/p\u003e\u003cp\u003eRolling Circle Amplification (RCA)\u003c\/p\u003e\u003cp\u003eWhole Genome Amplification (WGA)\u003c\/p\u003e\u003cp\u003eStrand Displacement Activity\u003c\/p\u003e\u003cp\u003eThe high processivity of the enzyme allows for efficient amplification of long DNA fragments up to 70,000 base insertions per binding event, while the high-fidelity ensures that the amplified DNA is of high quality and accuracy.\u003c\/p\u003e\u003cp\u003eFigure 1. Whole genome amplification as outlined in Protocol 2 of this manual. Five different lots of NxGen phi29 DNA Polymerase were used and duplicate NTC and (+) human genomic amplification reactions were set up. Amplification reactions were incubated for 16 hours at 30 °C and products were analysed in a 0.7% agarose gel and stained with ethidium bromide to visualise amplified DNA. The red arrows indicate NTC reactions with detectable background (input template independent) amplification.\u003c\/p\u003e\u003cp\u003eread more\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003ePowerful strand displacement activity, making it ideal for challenging samples or damaged DNA\u003c\/li\u003e\n\u003cli\u003eHighly processive, allowing for amplification of up to 70,000 base insertions per binding event\u003c\/li\u003e\n\u003cli\u003eHigh-fidelity 3'-\u0026gt;5' proofreading exonuclease function for accurate DNA replication with low error rates\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cp\u003eUnit Definition: 1 unit is defined as the amount of polymerase required to convert 0.5 pmol of dNTP's into acid insoluble material in 10 minutes at 30°C.\u003c\/p\u003e\u003cp\u003eSource: A recombinant E. coli strain carrying the phi29 DNA Polymerase gene from bacteriophage phi29.\u003c\/p\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eThe NxGen Phi29 DNA Polymerase sources its enzyme from a recombinant E. coli strain carrying the phi29 DNA polymerase gene from bacteriophage phi29.\u003c\/p\u003e\u003cp\u003e1 unit is defined as the amount of polymerase required to convert 0.5 pmol of dNTP's into acid insoluble material in 10 minutes at 30 °C.\u003c\/p\u003e\u003cp\u003eThe enzyme storage buffer consists of 10 mM Tris-HCl, 100 mM KCl, 0.1 mM EDTA, 1 mM dithiothreitol, 0.5% Tween-20, 0.5% NP-40, 50% glycerol, pH 7.4 @ 25 °C and the 10X phi29 DNA Polymerase Buffer is composed of 500 mM Tris-HCl, 100 mM (NH4)2SO4, 40 mM Dithiothreitol, 100 mM MgCl2, pH 7.5 @ 25 °C.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-011_30221-NxGen-phi29-DNA-Polymerase\"\u003e30221 NxGen phi29 DNA Polymerase\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_PCRAMP-011_phi29\"\u003ephi29\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/pis_PCRAMP-011_Enzyme-Catalog-Full\"\u003eEnzyme Catalog Full\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"10 U\/µL, 2,000 U","offer_id":45986430845007,"sku":"30221-1","price":1149.0,"currency_code":"ILS","in_stock":true},{"title":"10 U\/µL, 10,000 U","offer_id":45986430877775,"sku":"30221-2","price":4466.0,"currency_code":"ILS","in_stock":true},{"title":"50,000 U at 10,000 U\/mL","offer_id":45986430910543,"sku":"30221-3","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"5 mL at 10,000 U\/mL, 50,000 U","offer_id":45986430943311,"sku":"F83900-2","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/NxGen-phi29-DNA-Polymerase_2bd500de-592d-4cb3-8749-e6343fa17169.jpg?v=1785956103"},{"product_id":"nxgen-m-mulv-reverse-transcriptase-30222-1","title":"NxGen M-MuLV Reverse Transcriptase","description":"\u003cp\u003eM-MuLV Reverse Transcriptase is an RNA-dependent DNA polymerase which shows no measurable 3′→5′ proofreading function. This enzyme can copy a single-stranded DNA template or perform cDNA synthesis by extending a DNA primer annealed to an RNA template.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cp\u003eUnit Definition: 1 unit is defined as the amount of enzyme required to incorporate 1 nmol of dTTP into acid insoluble material in 10 minutes at 37 °C using poly r(A)\/oligo (dT) as a substrate.\u003c\/p\u003e\u003cp\u003eSource: A recombinant E. coli strain carrying the Moloney-Murine Leukemia Virus Reverse Transcriptase gene.\u003c\/p\u003e\u003cp\u003eUnit Concentration\t200,000 U\/mL\u003cbr\u003ePurity (SDS-PAGE)\t\u0026gt;99%\u003cbr\u003eSS Exonuclease\t2,000 U \u0026lt;5.0% released\u003cbr\u003eDS Exonuclease\t2,000 U \u0026lt;0.5% released\u003cbr\u003eEndonuclease\t2,000 U \u0026lt;10% converted\u003cbr\u003eE. coli 16S rDNA Contamination\t2,000 U \u0026lt;10 copies\u003cbr\u003eStorage\t-20 °C\u003cbr\u003eread more\u003c\/p\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eSupplied at 200,000 U\/mL in 50 mM Tris-HCl, 150 mM NaCl, 0.1 mM EDTA, 1 mM dithiothreitol, 0.1% NP-40 Alternative, 50% glycerol, pH 7.6 @ 25 °C. Also provided is 10X M-MuLV RT Buffer which in 1X form is composed of 50 mM Tris-HCl, 75 mM KCl, 3 mM MgCl2, 10 mM dithiothreitol, pH 8.3 @ 25 °C. 250,000 U is supplied as 5 × 50,000 U.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_30222-1_SDS-30222-NxGen-M-MuLV-Reverse-Transcriptase\"\u003eSDS 30222 NxGen M MuLV Reverse Transcriptase\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_30222-1_MA115-M-MuLV\"\u003eMA115 M MuLV\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/pis_30222-1_Enzyme-Catalog-Full\"\u003eEnzyme Catalog Full\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"Default Title","offer_id":45986431107151,"sku":"30222-1","price":1102.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/NxGen-M-MuLV-Reverse-Transcriptase_76af2cc0-8939-42c5-b17a-a067ba9a1869.jpg?v=1785956106"},{"product_id":"nxgen-t7-rna-polymerase-30223-1","title":"NxGen T7 RNA Polymerase","description":"\u003cp\u003eT7 RNA Polymerase catalyses the 5′→3′ RNA synthesis from the T7 promoter. It is a DNA-dependent RNA polymerase cloned from the T7 bacteriophage, which recognises the T7 promoter and terminator sequences with high specificity.\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\u003cli\u003eReliable transcription.\u003c\/li\u003e\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cp\u003eUnit Definition: One unit is defined as the amount of enzyme that will incorporate 1 nmol of ATP into acid-precipitable material in 1 hour at 37 °C.\u003c\/p\u003e\u003cp\u003eSource: Purified from a strain of E. coli that expresses the recombinant T7 RNA Polymerase gene.\u003c\/p\u003e\u003cp\u003eUnit Concentration\t50,000 U\/mL\u003cbr\u003ePurity (SDS-PAGE)\t\u0026gt;99%\u003cbr\u003eSS Exonuclease\t500 U \u0026lt;0.1% released\u003cbr\u003eDS Exonuclease\t500 U \u0026lt;0.1% released\u003cbr\u003eEndonuclease\t500 U \u0026lt;10% converted\u003cbr\u003eRNase Contamination\t500 U = none detected\u003cbr\u003eE. coli 16S rDNA Contamination\t500 U \u0026lt;10 copies\u003cbr\u003eStorage\t-20 °C\u003cbr\u003eread more\u003c\/p\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eSupplied at 50,000 U\/mL in 50 mM Tris-HCl, 100 mM NaCl, 1 mM dithiothreitol, 1 mM EDTA, 50% glycerol, 0.1% Triton X-100, pH 7.9 @ 25°C. Also provided is 10X T7 RNA Polymerase Buffer which in 1X form is composed of 40 mM Tris-HCl, 6 mM MgCl2, 10 mM Dithiothreitol, 2 mM Spermidine, pH 7.9 @ 25°C.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_30223-1_SDS-30223-NxGen-T7-RNA-Polymerase\"\u003eSDS 30223 NxGen T7 RNA Polymerase\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_30223-1_T7-RNAP\"\u003eT7 RNAP\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/pis_30223-1_Enzyme-Catalog-Full\"\u003eEnzyme Catalog Full\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"Default Title","offer_id":45986431205455,"sku":"30223-1","price":1015.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/NxGen-T7-RNA-Polymerase_27fb9e79-95df-4abb-90d3-e47cada8fbdd.jpg?v=1785956107"},{"product_id":"nxgen-t4-dna-ligase-nxgenz-001","title":"NxGen T4 DNA Ligase","description":"\u003cp\u003eT4 DNA Ligase catalyses the formation of a phosphodiester bond between the terminal 5′ phosphate and 3′ hydroxyl groups of duplex DNA or RNA. The enzyme efficiently joins blunt and cohesive ends and repairs single stranded nicks in duplex DNA, RNA or DNA\/RNA hybrids.\u003c\/p\u003e\u003cp\u003eT4 DNA Ligase Version\tSizes\tLigase Concentration\tProvided Buffer\u003cbr\u003eLow Concentration Ligase\u003c\/p\u003e\u003cp\u003e1,500 U\u003cbr\u003e7,500 U\u003c\/p\u003e\u003cp\u003e2 U\/µL\t10X T4 DNA Ligase Buffer\u003cbr\u003eHigh Concentration Rapid Kit\t1,500 U\u003cbr\u003e7,500 U\t10 U\/µL\t2X Rapid Ligation Buffer,\u003cbr\u003e10X T4 DNA Ligase Buffer\u003c\/p\u003e\u003cp\u003e10X T4 DNA Ligase Buffer is composed of 500 mM Tris-HCI, 100 mM MgCl 2, 50 mM dithiothreitol, 10 mM ATP, pH 7.6 @ 25 °C.\u003c\/p\u003e\u003cp\u003e2X Rapid Ligation Buffer is composed of 132 mM Tris-HCI, 20 mM MgCl 2, 2 mM dithiothreitol, 2 mM ATP, 15% PEG, pH 7.6 @ 25 °C.\u003c\/p\u003e\u003cp\u003eFigure 1: Purity\u003cbr\u003eThe purity of the T4 DNA ligase is equal or greater than 95% as judged by SDS-polyacrylamide gel electrophoresis with Coomassie blue staining. Molecular weight markers are in Lane 1. T4 DNA ligase was loaded at 5 units (Lane 2) and 10 units (Lane 3).\u003c\/p\u003e\u003cp\u003eFigure 2: Exonuclease and Endonuclease Activity Assay\u003cbr\u003eThe T4 DNA ligase is free of detectable of exo- and endonuclease activities, as judged by agarose gel electrophoresis following incubation of 10 units of enzyme for 16 hours at 37 °C with 1 µg of HindIII-digested λ DNA (Lanes 3 and 4) and supercoiled pUC19 DNA (Lanes 5 and 6). Molecular weight markers are in Lanes 1 and 2.\u003c\/p\u003e\u003cp\u003eread more\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\u003cli\u003eReliably join double-stranded nucleic acid chains.\u003c\/li\u003e\u003c\/ul\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eThe low concentration ligase includes NxGen T4 DNA Ligase at 2 U\/µL and 10X T4 DNA ligase Buffer. The high concentration ligase includes NxGen T4 DNA Ligase at 10 U\/µL and both 2X Rapid Ligation Buffer and 10X T4 DNA ligase buffer. Note - item ID 30243-3, NxGen T4 DNA Ligase (High Concentration) Kit, 10 U\/µL, 40,000 units includes T4 DNA Ligase and 10X T4 DNA ligase buffer only.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_NXGENZ-001_30241-NxGen-T4-DNA-Ligase-Low-Concentration\"\u003e30241 NxGen T4 DNA Ligase Low Concentration\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_NXGENZ-001_30243-NxGen-T4-DNA-Ligase-High-Concentration\"\u003e30243 NxGen T4 DNA Ligase High Concentration\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_NXGENZ-001_SDS-30241-NxGen-T4-DNA-Ligase-Low-Concentration\"\u003eSDS 30241 NxGen T4 DNA Ligase Low Concentration\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_NXGENZ-001_SDS-30243-NxGen-T4-DNA-Ligase-High-Concentration\"\u003eSDS 30243 NxGen T4 DNA Ligase High Concentration\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_NXGENZ-001_T4-Lig-HC\"\u003eT4 Lig HC\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_NXGENZ-001_T4-Lig-LC\"\u003eT4 Lig LC\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/pis_NXGENZ-001_Enzyme-Catalog-Full\"\u003eEnzyme Catalog Full\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"2 U\/µL, 1,500 U","offer_id":45986431270991,"sku":"30241-1","price":918.0,"currency_code":"ILS","in_stock":true},{"title":"10 U\/µL, 1,500 U","offer_id":45986431303759,"sku":"30243-1","price":1400.0,"currency_code":"ILS","in_stock":true},{"title":"10 U\/uL, 40,000 U","offer_id":45986431336527,"sku":"30243-3","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"30 U\/µL, 15,000 U","offer_id":45986431369295,"sku":"F836197","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"10 U\/µL, 40,000 U","offer_id":45986431402063,"sku":"F83914-2","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/NxGen-T4-DNA-Ligase_f9d7aaa5-4766-4010-a45b-002944a5ef88.jpg?v=1785956110"},{"product_id":"rapidxfire-thermostable-reverse-transcriptase-307","title":"RapiDxFire Thermostable Reverse Transcriptase","description":"\u003cp\u003eRapiDxFire Thermostable Reverse Transcriptase is a significant advancement over popular MMLV- and AMV reverse transcriptases. Originally identified in hot springs, this robust bacteriophage enzyme exhibits increasingly high activity at elevated temperatures (~60% activity remains after 10 min at 90 ⁰C) and maintains its core activity after \u0026gt;3 months storage at 20-25⁰C. This enzyme lacks RNAse H and 3’ 5’ exonuclease activity and efficiently synthesises short cDNA fragments (≤ 1 Kb). Like other reverse transcriptases, RapiDxFire RT possesses DNA polymerase activity but lacks 5′ 3′ exonuclease activity. Supplied in a glycerol-free, Triton™ X-100-free storage buffer, the enzyme formulation can be further optimised for downstream lyophilisation.\u003c\/p\u003e\u003cp\u003eRapiDxFire Thermostable RT Performs Best at High Temperatures\u003c\/p\u003e\u003cp\u003eFigure 1. Reaction temperature profile for RapiDxFire Thermostable Reverse Transcriptase. Reverse transcription reactions were set up on ice using each manufacturer’s recommended buffer system and Poly (rC) \/p(dG)12-18 template\/primer substrate. Reactions were transferred from ice to the indicated temperatures (37, 50, 55, 60, 65, 70, 75, and 80 °C) and incubated for 40 minutes. Following incubation, RNA\/cDNA heteroduplex product is quantified as a measure of polymerisation activity, utilising PicoGreen® fluorescence on a Tecan Infinite® M1000 Pro. RapiDxFire Thermostable RT exhibits increasing activity as the reaction temperature is increased, up to 80 °C (highest temperature tested); ~60% activity remains after 10 min at 90 °C (data not shown).\u003c\/p\u003e\u003cp\u003eSuperior Detection of Zika virus with RapiDxFire Thermostable RT\u003c\/p\u003e\u003cp\u003eFigure 2. cDNA synthesis time course studies in a 2-step RT-qPCR reaction with different thermostable RTs. A) qPCR curve after a 1 minute reverse transcription reaction. Zika cDNA synthesis was conducted for each enzyme in duplicate using target-specific primers and recommended reaction buffer and incubation temperatures per suppliers’ guidelines (RapiDxFire= 60 ⁰C, Supplier B= 50 ⁰C, and Supplier T= 55 ⁰C). After cDNA synthesis real time PCR was performed using one-tenth volume of each of the cDNA samples. PCR was performed using EconoTaq and its supplied buffer (Item No. 30031-3). Detection of PCR products were done real time using an intercalating dye (Dyomics Cat No. V13-01184) and BioRad CFX C1000 Touch™ with absorption\/emission of 481nm\/526nm. B) Reverse transcription time course study prior to performing second-step real-time PCR. Encircled data points are derived from data represented in the qPCR curve in A).\u003c\/p\u003e\u003cp\u003e3-month Room Temperature Stability\u003c\/p\u003e\u003cp\u003eFigure 3. RapiDxFire Thermostable RT was stored in separate aliquots per time point at ambient and -20 °C. At each time point RapiDxFire Thermostable RT was evaluated by measuring first-strand cDNA synthesis of 10000 copies MS2 RNA. cDNA synthesis was carried out using a gene-specific primer for MS2 at reaction temperature of 60 °C for 5 minutes. cDNA was measured using real time PCR and MS2 primers design around a 520 base pair amplicon.\u003c\/p\u003e\u003cp\u003eLegal Information\/Updates:\u003cbr\u003eUS Patent 8093030 for RapiDxFire™ is owned by LGC, Biosearch Technologies. For research use only. Not for use in diagnostic procedures.\u003c\/p\u003e\u003cp\u003eread more\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eExtremely active at high temperatures (55 °C to 80 °C): Improves specificity of cDNA Synthesis from diverse RNA templates\u003c\/li\u003e\n\u003cli\u003eSensitive: Detects 100 copies of RNA in two-step RT-qPCR assays\u003c\/li\u003e\n\u003cli\u003eShort reaction time (5 minutes or less): Streamlined RT-qPCR workflow and faster time to results\u003c\/li\u003e\n\u003cli\u003eStable at room temperature (\u0026gt; 3 months): Simplifies setup on automation decks (no cold storage required) and enables use in environments with limited cold-storage\u003c\/li\u003e\n\u003cli\u003eLyo-compatible: Enzyme formulation is free of glycerol -and other components- that are known to interfere with downstream lyophilisation\u003c\/li\u003e\n\u003cli\u003eBatch to batch reproducibility: Manufactured in an ISO 13485-certified facility\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cp\u003eStorage: Store at -20 °C.\u003c\/p\u003e\u003cp\u003eStorage Buffer: RapiDxFire Thermostable RT is supplied in a glycerol-free, Triton X-100-free buffer.\u003c\/p\u003e\u003cp\u003eUnit Definition: One RT unit is defined as the amount of enzyme required to incorporate 1 nmol of dTTP into an acid-insoluble form in 10 minutes at 55 °C (using poly(rA) oligo(dT) as template primer and 1X kit -supplied buffer formulation).\u003c\/p\u003e\u003cp\u003eFunctional QC Assay: RapiDxFire Thermostable RT is functionally tested with gene-° primer and bacteriophage MS2 RNA. In this reaction the enzyme converts 1000 copies of MS2 RNA into 520 bp cDNA in 5 minutes at 60 °C.\u003c\/p\u003e\u003cp\u003eEndonuclease: Free of contaminating endonucleases at the 10 Unit level.\u003c\/p\u003e\u003cp\u003eExonuclease: Free of contaminating exonucleases at the 10 Unit level.\u003c\/p\u003e\u003cp\u003eRNase Activity: No RNase activity detected using a fluorescence-based RNase activity assay.\u003c\/p\u003e\u003cp\u003eread more\u003c\/p\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eContents include RapiDxFire Thermostable Reverse Transcriptase (3 U\/µL) and 10X RapiDxFire Reaction Buffer. The enzyme pack sizes (50 or 250 Rxns) are based on 25 µL reaction volumes.\u003c\/p\u003e\n\u003ch2\u003eResources\u003c\/h2\u003e\n\u003cp\u003eBrochures and guides\u003c\/p\u003e\u003cp\u003eapp note rapidxfire reverse transcriptase\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_307_30250-RapidxFire-Thermostable-RT\"\u003e30250 RapidxFire Thermostable RT\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_307_RapiDxFire-reverse-transcriptase-manual\"\u003eRapiDxFire reverse transcriptase manual\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/guide_307_app-note-rapidxfire-reverse-transcriptase\"\u003eapp note rapidxfire reverse transcriptase\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"50 x 25 µL Reactions","offer_id":45986431467599,"sku":"30250-1","price":2770.0,"currency_code":"ILS","in_stock":true},{"title":"250 x 25 µL Reactions","offer_id":45986431500367,"sku":"30250-2","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/RapiDxFire-Thermostable-Reverse-Transcriptase_cbcc5f5c-0cfb-465a-ac15-09668a324b7d.jpg?v=1785956113"},{"product_id":"endonuclease-viii-ngs-010","title":"Endonuclease VIII","description":"\u003cp\u003eE.coli Endonuclease VIII is a bifunctional DNA glycosylase with DNA N-glycosylase and AP lyase activities. The N-glycosylase activity releases damaged pyrimidines from double-stranded DNA generating an apurinic (AP) site. The AP lyase activity cleaves the DNA phosphodiester backbone at AP sites via β- and δ-elimination, creating a one nucleotide gap with 5′ and 3′ phosphate termini. Damaged bases recognised and removed by Endonuclease VIII include thymine glycol, uracil glycol, urea, 5,6-dihydroxythymine, 5-hydroxy-5-methylhydantoin, 5-hydroxy-6-hydrothymine, 5,6-dihydrouracil and alloxan.\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e• Removes oxidatively damaged pyrimidines from DNA\u003c\/li\u003e\n\u003cli\u003e• Has both β and δ lyase activity, whereas Endonuclease III only has β lyase activity\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eEndonuclease VIII Kit contains Endonuclease VIII enzyme (10 U\/µL) and 10X Reaction Buffer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_NGS-010_Endonuclease-VIII\"\u003eEndonuclease VIII\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_NGS-010_Endonuclease-VIII-Reaction-Buffer-10X\"\u003eEndonuclease VIII Reaction Buffer 10X\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_NGS-010_E.-coli-Endonuclease-VIII-Kit\"\u003eE. coli Endonuclease VIII Kit\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"000 Units at 10","offer_id":45986431565903,"sku":"30270","price":486.0,"currency_code":"ILS","in_stock":true},{"title":"000 Units at 1","offer_id":45986431598671,"sku":"30271","price":3729.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/Endonuclease-VIII_653da74f-dbec-49be-87ce-33c49ce8d3f5.jpg?v=1785956114"},{"product_id":"nxgen-rnase-inhibitor-pcramp-016","title":"NxGen RNase Inhibitor","description":"\u003cp\u003eRNAse Inhibitor is a potent inhibitor of ribonucleases such as RNase A, RNase B, and RNase C. The 52 kDa protein is a fusion of the porcine RNAse Inhibitor gene with a proprietary 22.5 kDa protein tag.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cp\u003eUnit Definition: One unit is defined as the amount of enzyme required to inhibit by 50% the hydrolysis of cytidine 2',3'-cyclic monophosphate by 5 ng of RNAse A.\u003c\/p\u003e\u003cp\u003eSource: A recombinant E. coli strain carrying the porcine RNase Inhibitor gene.\u003c\/p\u003e\u003cp\u003eUnit Concentration\t40,000 U\/mL\u003cbr\u003ePurity (SDS-PAGE)\t\u0026gt;99%\u003cbr\u003eSS Exonuclease\t2000 U \u0026lt;5.0% released\u003cbr\u003eDS Exonuclease\t2000 U \u0026lt;0.5% released\u003cbr\u003eEndonuclease\t2000 U \u0026lt;10% converted\u003cbr\u003eNon-specific RNAse\t2000 U none detected\u003cbr\u003eE. coli 16S rDNA Contamination\t2000 U \u0026lt;10 copies\u003cbr\u003eStorage\t-20 °C\u003cbr\u003eread more\u003c\/p\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eSupplied at 40,000 U\/mL in 20 mM HEPES-KOH, 50 mM KCl, 8 mM dithiothreitol, 50% glycerol, pH 7.5 @ 25°C.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-016_30281-NxGen-RNase-Inhibitor\"\u003e30281 NxGen RNase Inhibitor\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_PCRAMP-016_RNAs-Inh\"\u003eRNAs Inh\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/pis_PCRAMP-016_Enzyme-Catalog-Full\"\u003eEnzyme Catalog Full\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"40 U\/µL, 10,000 U","offer_id":45986431729743,"sku":"30281-1","price":1521.0,"currency_code":"ILS","in_stock":true},{"title":"40 U\/µL, 50,000 U","offer_id":45986431762511,"sku":"30281-2","price":6332.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/NxGen-RNase-Inhibitor_46b7dcc5-fef8-4302-a7f5-36ffb565c412.jpg?v=1785956117"},{"product_id":"e-cloni-10g-bac-optimized-electrocompetent-cells-60215","title":"E. cloni 10G BAC-Optimized Electrocompetent Cells","description":"\u003cp\u003eWe offer two bacterial strains designed for BAC library construction or transformation of large constructs:\u003c\/p\u003e\u003cp\u003eBAC-Optimised Replicator™ v2.0 Electrocompetent Cells (\u0026gt; 1 × 1010 cfu\/µg pKanR DNA). These cells are required for on-demand, copy number amplification of the pSMART® BAC or pEZ™ BAC vectors in the CopyRight® Kits, thereby increasing DNA yields. BAC-Optimised Replicator Cells are components of CopyRight® Cloning Kits.\u003c\/p\u003e\u003cp\u003eE. cloni 10G BAC-Optimised Electrocompetent Cells (\u0026gt; 1 × 107 cfu\/µg 150 kb BAC DNA; \u0026gt; 1 × 1010 cfu\/µg pUC DNA). The first competent cells developed specifically for large DNA cloning. Manufactured using a novel procedure that maximises transformation efficiencies with very large inserts, 10G BAC-Optimised Cells are an excellent choice for BAC library construction and large construct transformation.\u003c\/p\u003e\u003cp\u003eTable 1. E. cloni Competent Cells for BAC or Large Fragment Cloning\u003c\/p\u003e\u003cp\u003eE. cloni Cell Lines\u003c\/p\u003e\u003cp\u003eTransformation Efficiency\u003cbr\u003e(cfu\/µg pUC or pKanR DNA)\u003c\/p\u003e\u003cp\u003eCloning Methylated DNA\u003c\/p\u003e\u003cp\u003eBAC, Cosmid Cloning\u003c\/p\u003e\u003cp\u003eBlue\/White Screening\u003c\/p\u003e\u003cp\u003e10G BAC-Optimised Electrocompetent\u003c\/p\u003e\u003cp\u003e\u0026gt;1 × 1010\u003c\/p\u003e\u003cp\u003eYES\u003c\/p\u003e\u003cp\u003eYES\u003c\/p\u003e\u003cp\u003eYES\u003cbr\u003ewithout IPTG induction\u003c\/p\u003e\u003cp\u003eBAC-Optimised Replicator v2.0 Electrocompetent\u003c\/p\u003e\u003cp\u003e\u0026gt;1 × 1010\u003c\/p\u003e\u003cp\u003eYES\u003c\/p\u003e\u003cp\u003eYES\u003c\/p\u003e\u003cp\u003eYES\u003cbr\u003eIPTG induction required\u003c\/p\u003e\u003cp\u003eread more\u003c\/p\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eCompetent Cells include Control DNA and Recovery Medium, and are packaged as FIVERs (5 transformations per tube), as indicated. Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_60215-2_SDS-60215-E.-cloni-10G-BAC-Optimized-Electrocompetent-Cells\"\u003eSDS 60215 E. cloni 10G BAC Optimized Electrocompetent Cells\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_60215-2_BAC-Optimized-Replicator\"\u003eBAC Optimized Replicator\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_60215-2_BAC-Optimized-Replicator-v2.0-Electrocompetent-Cells\"\u003eBAC Optimized Replicator v2.0 Electrocompetent Cells\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_60215-2_E-cloni-10-G-BAC-Optimized-Electro\"\u003eE cloni 10 G BAC Optimized Electro\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"12 Rxns (DUOs)","offer_id":45986431860815,"sku":"60061-1","price":1139.0,"currency_code":"ILS","in_stock":true},{"title":"24 Reactions (DUOs)","offer_id":45986431893583,"sku":"60061-2","price":1946.0,"currency_code":"ILS","in_stock":true},{"title":"25 rxns (Fivers)","offer_id":45986431926351,"sku":"60215-2","price":2218.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/BAC-Optimised-Electrocompetent-Cells_467be3c9-7a5c-4fa4-81fc-d7566b5bdc05.jpg?v=1785956120"},{"product_id":"e-cloni-10g-elite-electrocompetent-cells-efficiency-2-x-1010-cfu-g-comcel-019","title":"E. cloni 10G Elite Electrocompetent Cells, Efficiency \u003e2 x 1010 cfu\/µg","description":"\u003cp\u003eE. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5α\u0026amp;trade, DH10B\u0026amp;trade, JM109, TOP10, etc. and directly replace them in cloning protocols. However, E. cloni electrocompetent cells incorporate a unique manufacturing technology that increases transformation efficiency, recombinant yields and reliability (Figures. 1 and 2), while decreasing costs. These cells provide solutions for a wide range of applications at economical prices.\u003c\/p\u003e\u003cp\u003eChoice of strain:\u003cbr\u003eE. cloni 10G Competent Cells. Library construction, cloning, subcloning, and plasmid isolation with or without blue\/white screening.\u003cbr\u003eE. cloni 10GF′ Competent Cells. Contain the F' plasmid for infection with M13 to produce ssDNA.\u003cbr\u003eChoice of efficiency:\u003cbr\u003eE. cloni 10G SUPREME Electrocompetent Cells \u0026gt; 4 × 1010 cfu\/µg pUC DNA\u003cbr\u003eSUPREME Cells have the highest transformation efficiency available from any supplier. Choose SUPREME Cells for the most demanding cloning situations, such as construction of large, high complexity libraries or cloning difficult targets, which require the greatest number of transformants possible.\u003cbr\u003eE. cloni 10G \u0026amp; 10GF´ ELITE Electrocompetent Cells \u0026gt; 2 × 1010 cfu\/µg pUC DNA\u003cbr\u003eELITE Cells have twice the transformation efficiency compared to “ultra high efficiency” cells from other suppliers. ELITE Cells provide large numbers of transformants from hard-to-clone fragments or limited DNA at a lower price.\u003cbr\u003eE. cloni 10G CLASSIC Electrocompetent Cells \u0026gt; 5 × 109 cfu\/µg pUC DNA\u003cbr\u003eCLASSIC Cells are high efficiency cells with a substantially lower cost per reaction. These cells are the most economical choice for standard cloning and library construction. 10G CLASSIC Cells are available in larger package sizes for convenient use in higher volume cloning applications.\u003c\/p\u003e\u003cp\u003eCustom Competent Cells\u003c\/p\u003e\u003cp\u003eBulk cells or your strain of E. coli, made to the highest possible transfection efficiency with custom dispensing! See Custom Competent Cells page for detailed information.\u003c\/p\u003e\u003cp\u003ePlasmid cloning. E. cloni 10G SUPREME Cells provide recombinant yields higher than the highest efficiency cells offered by a leading supplier (Figures. 1 and 2)\u003c\/p\u003e\u003cp\u003eFigure 1. E. cloni 10G SUPREME Electrocompetent Cells consistently outperformed “ultra high efficiency” cells from a leading supplier. Both strains were transformed with 10 pg of pUC19 (n=16).\u003c\/p\u003e\u003cp\u003eFigure 2.Transformation efficiency comparison of E. cloni 10G SUPREME Cells and Mega DH10B cells (Invitrogen). Competent cells were transformed according to the manufacturer’s instructions with the same pUC19 DNA control (provided with the Invitrogen cells)\u003c\/p\u003e\u003cp\u003eTable 1. E. cloni Competent Cells for Cloning and Library Construction\u003c\/p\u003e\u003cp\u003eE. cloni Cell Lines\u003c\/p\u003e\u003cp\u003eTransformation Efficiency\u003cbr\u003e(cfu\/µg pUC DNA)\u003c\/p\u003e\u003cp\u003eCloning Methylated DNA\u003c\/p\u003e\u003cp\u003eBAC, Cosmid Cloning\u003c\/p\u003e\u003cp\u003eBlue\/White Screening\u003c\/p\u003e\u003cp\u003e10G SUPREME Electrocompetent\u003c\/p\u003e\u003cp\u003e\u0026gt;4 × 1010\u003c\/p\u003e\u003cp\u003eYES\u003c\/p\u003e\u003cp\u003eYES\u003c\/p\u003e\u003cp\u003eYES\u003cbr\u003ewithout IPTG induction\u003c\/p\u003e\u003cp\u003e10G ELITE Electrocompetent\u003c\/p\u003e\u003cp\u003e\u0026gt;2 × 1010\u003c\/p\u003e\u003cp\u003eYES\u003c\/p\u003e\u003cp\u003eNO\u003c\/p\u003e\u003cp\u003eYES\u003cbr\u003ewithout IPTG induction\u003c\/p\u003e\u003cp\u003e10G CLASSIC Electrocompetent\u003c\/p\u003e\u003cp\u003e\u0026gt;5 × 109\u003c\/p\u003e\u003cp\u003eYES\u003c\/p\u003e\u003cp\u003eNO\u003c\/p\u003e\u003cp\u003eYES\u003cbr\u003ewithout IPTG induction\u003c\/p\u003e\u003cp\u003e10GF´ ELITE Electrocompetent\u003c\/p\u003e\u003cp\u003e\u0026gt;2 × 1010\u003c\/p\u003e\u003cp\u003eYES\u003c\/p\u003e\u003cp\u003eNO\u003c\/p\u003e\u003cp\u003eYES\u003cbr\u003eIPTG induction required\u003c\/p\u003e\u003cp\u003eread more\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eDirect replacements for standard cloning strains\u003c\/li\u003e\n\u003cli\u003eOptimised genetics for high yields: phage T1 resistant, endonuclease and recombination minus, blue\/white screening-capable.\u003c\/li\u003e\n\u003cli\u003eAvailable in a range of transformation efficiencies .\u003c\/li\u003e\n\u003cli\u003eConvenient packaging options.\u003c\/li\u003e\n\u003cli\u003eHigh efficiency cells result in high colony counts\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eCompetent Cells include Control DNA and Recovery Medium, and are packaged as SOLOs (1 transformation per tube), DUOs (2 transformations per tube), Subcloning Grade (12 transformations per tube), or microplates as indicated. Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_COMCEL-019_60051-1-E-cloni-10G-ELITE-Electrocompetent-Cells-solo\"\u003e60051 1 E cloni 10G ELITE Electrocompetent Cells solo\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_COMCEL-019_60052-0-60052-1-60052-2-60052-3-60052-4-E-cloni-10G-ELITE-Electrocompetent-Cells-Duo-Sixpack\"\u003e60052 0 60052 1 60052 2 60052 3 60052 4 E cloni 10G ELITE Electrocompetent Cells Duo Sixpack\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_COMCEL-019_60061-1-60061-2-E-cloni-10GF-Prime-Electrocompetent-Cells\"\u003e60061 1 60061 2 E cloni 10GF Prime Electrocompetent Cells\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_COMCEL-019_60080-1-60082-2-E-cloni-10G-Supreme-Electrocompetent-Cells-Duo\"\u003e60080 1 60082 2 E cloni 10G Supreme Electrocompetent Cells Duo\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_COMCEL-001_60080-E.-cloni-10G-Supreme-Electrocompetent-Cells-Duo\"\u003e60080 E. cloni 10G Supreme Electrocompetent Cells Duo\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_COMCEL-001_60081-E.-cloni-10G-Supreme-Electrocompetent-Cells-Solo\"\u003e60081 E. cloni 10G Supreme Electrocompetent Cells Solo\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_COMCEL-001_60106-E.-cloni-Chemically-Competent-Cells-Solo\"\u003e60106 E. cloni Chemically Competent Cells Solo\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_COMCEL-019_60107-E\"\u003e60107 E\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_COMCEL-001_60107-E.-cloni-Chemically-Competent-Cells-Duos\"\u003e60107 E. cloni Chemically Competent Cells Duos\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_COMCEL-001_60108-E.-cloni-Chemically-Competent-Cells-Subcloning\"\u003e60108 E. cloni Chemically Competent Cells Subcloning\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_COMCEL-019_E-cloni-10GF-Prime-Electrocompetent-Cells\"\u003eE cloni 10GF Prime Electrocompetent Cells\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_COMCEL-019_Ecloni-10G-10GF'-Electrocompetent\"\u003eEcloni 10G 10GF' Electrocompetent\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_COMCEL-019_Ecloni-10G-Chem-Comp\"\u003eEcloni 10G Chem Comp\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_COMCEL-019_Ecloni-96-well-10G-ChemComp\"\u003eEcloni 96 well 10G ChemComp\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/pis_COMCEL-019_F300-US-ecloni-comp-cells-cloning\"\u003eF300 US ecloni comp cells cloning\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"12 Reactions (SOLOs)","offer_id":45986431991887,"sku":"60051-1","price":1270.0,"currency_code":"ILS","in_stock":true},{"title":"12 Reactions (DUOs)","offer_id":45986432024655,"sku":"60052-1","price":1119.0,"currency_code":"ILS","in_stock":true},{"title":"24 Reactions (DUOs)","offer_id":45986432057423,"sku":"60052-2","price":1913.0,"currency_code":"ILS","in_stock":true},{"title":"24 Reactions (SixPacks)","offer_id":45986432090191,"sku":"60052-3","price":1481.0,"currency_code":"ILS","in_stock":true},{"title":"48 Reactions (SixPacks)","offer_id":45986432122959,"sku":"60052-4","price":2442.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/E-cloni-10G-Elite-Electrocompetent-Cells_252C-Efficiency-_253E2-x-1010-cfu-_25C2_25B5g_c43476c9-de8a-4d40-b3bd-c1928cf7f2b4.jpg?v=1785956121"},{"product_id":"e-cloni-10g-supreme-electrocompetent-cells-efficiency-4-x-1010-cfu-g-comcel-018","title":"E. cloni 10G Supreme Electrocompetent Cells, Efficiency \u003e4 x 1010 cfu\/µg","description":"\u003cp\u003eE. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5α\u0026amp;trade, DH10B\u0026amp;trade, JM109, TOP10, etc. and directly replace them in cloning protocols. However, E. cloni electrocompetent cells incorporate a unique manufacturing technology that increases transformation efficiency, recombinant yields and reliability (Figures. 1 and 2), while decreasing costs. These cells provide solutions for a wide range of applications at economical prices.\u003c\/p\u003e\u003cp\u003eChoice of strain:\u003cbr\u003eE. cloni 10G Competent Cells. Library construction, cloning, subcloning, and plasmid isolation with or without blue\/white screening.\u003cbr\u003eE. cloni 10GF′ Competent Cells. Contain the F' plasmid for infection with M13 to produce ssDNA.\u003cbr\u003eChoice of efficiency:\u003cbr\u003eE. cloni 10G SUPREME Electrocompetent Cells \u0026gt; 4 × 1010 cfu\/µg pUC DNA\u003cbr\u003eSUPREME Cells have the highest transformation efficiency available from any supplier. Choose SUPREME Cells for the most demanding cloning situations, such as construction of large, high complexity libraries or cloning difficult targets, which require the greatest number of transformants possible.\u003cbr\u003eE. cloni 10G \u0026amp; 10GF´ ELITE Electrocompetent Cells \u0026gt; 2 × 1010 cfu\/µg pUC DNA\u003cbr\u003eELITE Cells have twice the transformation efficiency compared to “ultra high efficiency” cells from other suppliers. ELITE Cells provide large numbers of transformants from hard-to-clone fragments or limited DNA at a lower price.\u003cbr\u003eE. cloni 10G CLASSIC Electrocompetent Cells \u0026gt; 5 × 109 cfu\/µg pUC DNA\u003cbr\u003eCLASSIC Cells are high efficiency cells with a substantially lower cost per reaction. These cells are the most economical choice for standard cloning and library construction. 10G CLASSIC Cells are available in larger package sizes for convenient use in higher volume cloning applications.\u003c\/p\u003e\u003cp\u003eCustom Competent Cells\u003c\/p\u003e\u003cp\u003eBulk cells or your strain of E. coli, made to the highest possible transfection efficiency with custom dispensing! See Custom Competent Cells page for detailed information.\u003c\/p\u003e\u003cp\u003ePlasmid cloning. E. cloni 10G SUPREME Cells provide recombinant yields higher than the highest efficiency cells offered by a leading supplier (Figures. 1 and 2)\u003c\/p\u003e\u003cp\u003eFigure 1. E. cloni 10G SUPREME Electrocompetent Cells consistently outperformed “ultra high efficiency” cells from a leading supplier. Both strains were transformed with 10 pg of pUC19 (n=16).\u003c\/p\u003e\u003cp\u003eFigure 2.Transformation efficiency comparison of E. cloni 10G SUPREME Cells and Mega DH10B cells (Invitrogen). Competent cells were transformed according to the manufacturer’s instructions with the same pUC19 DNA control (provided with the Invitrogen cells)\u003c\/p\u003e\u003cp\u003eTable 1. E. cloni Competent Cells for Cloning and Library Construction\u003c\/p\u003e\u003cp\u003eE. cloni Cell Lines\u003c\/p\u003e\u003cp\u003eTransformation Efficiency\u003cbr\u003e(cfu\/µg pUC DNA)\u003c\/p\u003e\u003cp\u003eCloning Methylated DNA\u003c\/p\u003e\u003cp\u003eBAC, Cosmid Cloning\u003c\/p\u003e\u003cp\u003eBlue\/White Screening\u003c\/p\u003e\u003cp\u003e10G SUPREME Electrocompetent\u003c\/p\u003e\u003cp\u003e\u0026gt;4 × 1010\u003c\/p\u003e\u003cp\u003eYES\u003c\/p\u003e\u003cp\u003eYES\u003c\/p\u003e\u003cp\u003eYES\u003cbr\u003ewithout IPTG induction\u003c\/p\u003e\u003cp\u003e10G ELITE Electrocompetent\u003c\/p\u003e\u003cp\u003e\u0026gt;2 × 1010\u003c\/p\u003e\u003cp\u003eYES\u003c\/p\u003e\u003cp\u003eNO\u003c\/p\u003e\u003cp\u003eYES\u003cbr\u003ewithout IPTG induction\u003c\/p\u003e\u003cp\u003e10G CLASSIC Electrocompetent\u003c\/p\u003e\u003cp\u003e\u0026gt;5 × 109\u003c\/p\u003e\u003cp\u003eYES\u003c\/p\u003e\u003cp\u003eNO\u003c\/p\u003e\u003cp\u003eYES\u003cbr\u003ewithout IPTG induction\u003c\/p\u003e\u003cp\u003e10GF´ ELITE Electrocompetent\u003c\/p\u003e\u003cp\u003e\u0026gt;2 × 1010\u003c\/p\u003e\u003cp\u003eYES\u003c\/p\u003e\u003cp\u003eNO\u003c\/p\u003e\u003cp\u003eYES\u003cbr\u003eIPTG induction required\u003c\/p\u003e\u003cp\u003eread more\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eDirect replacements for standard cloning strains\u003c\/li\u003e\n\u003cli\u003eOptimised genetics for high yields: phage T1 resistant, endonuclease and recombination minus, blue\/white screening-capable.\u003c\/li\u003e\n\u003cli\u003eAvailable in a range of transformation efficiencies .\u003c\/li\u003e\n\u003cli\u003eConvenient packaging options.\u003c\/li\u003e\n\u003cli\u003eHigh efficiency cells result in high colony counts\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eCompetent Cells include Control DNA and Recovery Medium, and are packaged as SOLOs (1 transformation per tube), DUOs (2 transformations per tube), Subcloning Grade (12 transformations per tube), or microplates as indicated. Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.\u003c\/p\u003e","brand":"Biosearch Technologies","offers":[{"title":"12 Reactions (DUOs)","offer_id":45986432188495,"sku":"60080-1","price":1735.0,"currency_code":"ILS","in_stock":true},{"title":"24 Reactions (DUOs)","offer_id":45986432221263,"sku":"60080-2","price":3179.0,"currency_code":"ILS","in_stock":true},{"title":"12 Reactions (SOLOs)","offer_id":45986432254031,"sku":"60081-1","price":1977.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/E-cloni-10G-Supreme-Electrocompetent-Cells_252C-Efficiency-_253E4-x-1010-cfu-_25C2_25B5g_97055989-3b19-42bd-8f14-a8c547f3dc60.jpg?v=1785956125"},{"product_id":"e-cloni-10g-chemically-competent-cells-comcel-001","title":"E. cloni 10G Chemically Competent Cells","description":"\u003cp\u003eE. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5α\u0026amp;trade, DH10B\u0026amp;trade, JM109, TOP10, etc. and directly replace them in cloning protocols. However, E. cloni electrocompetent cells incorporate a unique manufacturing technology that increases transformation efficiency, recombinant yields and reliability (Figures. 1 and 2), while decreasing costs. These cells provide solutions for a wide range of applications at economical prices.\u003c\/p\u003e\u003cp\u003eChoice of strain:\u003cbr\u003eE. cloni 10G Competent Cells. Library construction, cloning, subcloning, and plasmid isolation with or without blue\/white screening.\u003cbr\u003eE. cloni 10GF′ Competent Cells. Contain the F' plasmid for infection with M13 to produce ssDNA.\u003cbr\u003eChoice of efficiency:\u003cbr\u003eE. cloni 10G SUPREME Electrocompetent Cells \u0026gt; 4 × 1010 cfu\/µg pUC DNA\u003cbr\u003eSUPREME Cells have the highest transformation efficiency available from any supplier. Choose SUPREME Cells for the most demanding cloning situations, such as construction of large, high complexity libraries or cloning difficult targets, which require the greatest number of transformants possible.\u003cbr\u003eE. cloni 10G \u0026amp; 10GF´ ELITE Electrocompetent Cells \u0026gt; 2 × 1010 cfu\/µg pUC DNA\u003cbr\u003eELITE Cells have twice the transformation efficiency compared to “ultra high efficiency” cells from other suppliers. ELITE Cells provide large numbers of transformants from hard-to-clone fragments or limited DNA at a lower price.\u003cbr\u003eE. cloni 10G CLASSIC Electrocompetent Cells \u0026gt; 5 × 109 cfu\/µg pUC DNA\u003cbr\u003eCLASSIC Cells are high efficiency cells with a substantially lower cost per reaction. These cells are the most economical choice for standard cloning and library construction. 10G CLASSIC Cells are available in larger package sizes for convenient use in higher volume cloning applications.\u003c\/p\u003e\u003cp\u003eCustom Competent Cells\u003c\/p\u003e\u003cp\u003eBulk cells or your strain of E. coli, made to the highest possible transfection efficiency with custom dispensing! See Custom Competent Cells page for detailed information.\u003c\/p\u003e\u003cp\u003ePlasmid cloning. E. cloni 10G SUPREME Cells provide recombinant yields higher than the highest efficiency cells offered by a leading supplier (Figures 1 and 2)\u003c\/p\u003e\u003cp\u003eFigure 1. E. cloni 10G SUPREME Electrocompetent Cells consistently outperformed “ultra high efficiency” cells from a leading supplier. Both strains were transformed with 10 pg of pUC19 (n=16).\u003c\/p\u003e\u003cp\u003eFigure 2.Transformation efficiency comparison of E. cloni 10G SUPREME Cells and Mega DH10B cells (Invitrogen). Competent cells were transformed according to the manufacturer’s instructions with the same pUC19 DNA control (provided with the Invitrogen cells)\u003c\/p\u003e\u003cp\u003eTable 1. E. cloni Competent Cells for Cloning and Library Construction\u003c\/p\u003e\u003cp\u003eE. cloni Cell Lines\u003c\/p\u003e\u003cp\u003eTransformation Efficiency\u003cbr\u003e(cfu\/µg pUC DNA)\u003c\/p\u003e\u003cp\u003eCloning Methylated DNA\u003c\/p\u003e\u003cp\u003eBAC, Cosmid Cloning\u003c\/p\u003e\u003cp\u003eBlue\/White Screening\u003c\/p\u003e\u003cp\u003e10G SUPREME Electrocompetent\u003c\/p\u003e\u003cp\u003e\u0026gt;4 × 1010\u003c\/p\u003e\u003cp\u003eYES\u003c\/p\u003e\u003cp\u003eYES\u003c\/p\u003e\u003cp\u003eYES\u003cbr\u003ewithout IPTG induction\u003c\/p\u003e\u003cp\u003e10G ELITE Electrocompetent\u003c\/p\u003e\u003cp\u003e\u0026gt;2 × 1010\u003c\/p\u003e\u003cp\u003eYES\u003c\/p\u003e\u003cp\u003eNO\u003c\/p\u003e\u003cp\u003eYES\u003cbr\u003ewithout IPTG induction\u003c\/p\u003e\u003cp\u003e10G CLASSIC Electrocompetent\u003c\/p\u003e\u003cp\u003e\u0026gt;5 × 109\u003c\/p\u003e\u003cp\u003eYES\u003c\/p\u003e\u003cp\u003eNO\u003c\/p\u003e\u003cp\u003eYES\u003cbr\u003ewithout IPTG induction\u003c\/p\u003e\u003cp\u003e10GF´ ELITE Electrocompetent\u003c\/p\u003e\u003cp\u003e\u0026gt;2 × 1010\u003c\/p\u003e\u003cp\u003eYES\u003c\/p\u003e\u003cp\u003eNO\u003c\/p\u003e\u003cp\u003eYES\u003cbr\u003eIPTG induction required\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eDirect replacements for standard cloning strains\u003c\/li\u003e\n\u003cli\u003eOptimised genetics for high yields: phage T1 resistant, endonuclease and recombination minus, blue\/white screening-capable.\u003c\/li\u003e\n\u003cli\u003eAvailable in a range of high transformation efficiencies\u003c\/li\u003e\n\u003cli\u003eConvenient packaging options.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cp\u003eGenotypes\u003c\/p\u003e\u003cp\u003eE. cloni 10G: F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697galU galK rpsL nupG λ- tonA (StrR)\u003c\/p\u003e\u003cp\u003eE. cloni 10GF´: [F´ pro A+B+ lacIqZΔM15::Tn10 (TetR)] \/mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara, leu)7697 galU galK rpsL nupG λ- tonA (StrR)\u003c\/p\u003e\u003cp\u003eEfficiencies\u003c\/p\u003e\u003cp\u003eE. cloni 10G SUPREME Electrocompetent Cells \u0026gt; 4 × 1010 cfu\/µg pUC DNA\u003c\/p\u003e\u003cp\u003eE. cloni 10G \u0026amp; 10GF´ ELITE Electrocompetent Cells \u0026gt; 2 × 1010 cfu\/µg pUC DNA\u003c\/p\u003e\u003cp\u003eE. cloni 10G CLASSIC Electrocompetent Cells \u0026gt; 5 × 109 cfu\/µg pUC DNA\u003c\/p\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eCompetent Cells include Control DNA and Recovery Medium, and are packaged as SOLOs (1 transformation per tube), DUOs (2 transformations per tube), Subcloning Grade (12 transformations per tube), or microplates as indicated. Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_COMCEL-001_60051-1-E-cloni-10G-ELITE-Electrocompetent-Cells-solo\"\u003e60051 1 E cloni 10G ELITE Electrocompetent Cells solo\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_COMCEL-001_60052-0-60052-1-60052-2-60052-3-60052-4-E-cloni-10G-ELITE-Electrocompetent-Cells-Duo-Sixpack\"\u003e60052 0 60052 1 60052 2 60052 3 60052 4 E cloni 10G ELITE Electrocompetent Cells Duo Sixpack\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_COMCEL-001_60061-1-60061-2-E-cloni-10GF-Prime-Electrocompetent-Cells\"\u003e60061 1 60061 2 E cloni 10GF Prime Electrocompetent Cells\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_COMCEL-001_60061-E.-cloni-10GF-Prime-Electrocompetent-Cells\"\u003e60061 E. cloni 10GF Prime Electrocompetent Cells\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_COMCEL-001_60080-1-60082-2-E-cloni-10G-Supreme-Electrocompetent-Cells-Duo\"\u003e60080 1 60082 2 E cloni 10G Supreme Electrocompetent Cells Duo\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_COMCEL-001_60080-E.-cloni-10G-Supreme-Electrocompetent-Cells-Duo\"\u003e60080 E. cloni 10G Supreme Electrocompetent Cells Duo\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_COMCEL-001_60081-E.-cloni-10G-Supreme-Electrocompetent-Cells-Solo\"\u003e60081 E. cloni 10G Supreme Electrocompetent Cells Solo\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_COMCEL-001_60096-1-60096-4-E-cloni-96-well-Chemically-Competent-Cells\"\u003e60096 1 60096 4 E cloni 96 well Chemically Competent Cells\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_COMCEL-001_60106-E.-cloni-Chemically-Competent-Cells-Solo\"\u003e60106 E. cloni Chemically Competent Cells Solo\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_COMCEL-001_60107-E.-cloni-Chemically-Competent-Cells-Duos\"\u003e60107 E. cloni Chemically Competent Cells Duos\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_COMCEL-001_60108-E.-cloni-Chemically-Competent-Cells-Subcloning\"\u003e60108 E. cloni Chemically Competent Cells Subcloning\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_COMCEL-001_60117-E.-cloni-10G-Classic-Electrocompetent-Cells\"\u003e60117 E. cloni 10G Classic Electrocompetent Cells\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_COMCEL-001_Ecloni-10G-10GF'-Electrocompetent\"\u003eEcloni 10G 10GF' Electrocompetent\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_COMCEL-001_Ecloni-10G-Chem-Comp\"\u003eEcloni 10G Chem Comp\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_COMCEL-001_Ecloni-96-well-10G-ChemComp\"\u003eEcloni 96 well 10G ChemComp\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/pis_COMCEL-001_F300-US-ecloni-comp-cells-cloning\"\u003eF300 US ecloni comp cells cloning\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"4 plates","offer_id":45986432319567,"sku":"60096-4","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"12 Reactions (SOLOs)","offer_id":45986432352335,"sku":"60106-1","price":958.0,"currency_code":"ILS","in_stock":true},{"title":"12 Rxns (DUOs)","offer_id":45986432385103,"sku":"60107-1","price":827.0,"currency_code":"ILS","in_stock":true},{"title":"24 Reactions (DUOs)","offer_id":45986432417871,"sku":"60107-2","price":1447.0,"currency_code":"ILS","in_stock":true},{"title":"Subcloning Grade, 48 Reactions","offer_id":45986432450639,"sku":"60108-1","price":482.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/E-cloni-10G-Chemically-Competent-Cells_8bf44e7a-265f-49c3-aac1-8fde11cff9e8.jpg?v=1785956126"},{"product_id":"hi-control-10g-chemically-competent-cells-60110-1","title":"HI-Control 10G Chemically Competent Cells","description":"\u003cp\u003eThe HI-Control™ strains are based on E. cloni 10G ( DH10B) and BL21(DE3) strains. These strains harbor a plasmid bearing a constitutive lacI repressor gene, which provides tight control over promoters containing the lacO operator.\u003c\/p\u003e\u003cp\u003eTightly Control Protein Expression\t \t \u003cbr\u003e \tT7 Promoters\tNon-T7 lac promoters\u003cbr\u003e(T5-lac, tac, trc, lac)\tClone with low background target expression\tTransformation efficiencies\u003cbr\u003eHI-Control\u003cbr\u003eBL21(DE3)\tX\tX\t \t≥ 1×107 cfu\/µg\u003cbr\u003eHI-Control\u003cbr\u003e10G\t \tX\tX\t≥ 1×109 cfu\/µg\u003cbr\u003eGenotypes:\u003c\/p\u003e\u003cp\u003eHI-Control BL21 (DE3): F- ompT hsdSB (rB- mB-) gal dcm (DE3) Mini-F lacIq1(GentR)\u003c\/p\u003e\u003cp\u003eHI-Control 10G: mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 ɸ80dlacZΔM15 ΔlacX74 araD139 Δ (ara,leu)7697 galU galK rpsL (StrR) nupG λ− tonA Mini-F lacIq1 (GentR)\u003c\/p\u003e\u003cp\u003eread more\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eReduce leaky expression: The LacI repressor protein binds to the lacO operator, blocking transcription of downstream genes. In the absence of an induction agent such as lactose sugar or IPTG, target protein expression from the adjacent promoter is greatly reduced.\u003c\/li\u003e\n\u003cli\u003eObtain high protein yields: Both 10G and BL21(DE3)-based strains express high levels of protein. For expression from a T7 promoter, select the HI-Control BL21(DE3) strain. Other lac-based promoters like T5-lac, tac, trc, and lac, can be used in either HI-Control strain.\u003c\/li\u003e\n\u003cli\u003eAchieve high efficiency transformations: Highly efficient competent cells give excellent results from small amounts of plasmid DNA.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cp\u003ePlease note that the SUMO Protease and SUMO Fusion tag are modified from the original native proteins. Native or non-modified control proteins or proteases will not be compatible.\u003c\/p\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eCompetent Cells include Control DNA and Recovery Medium, and are packaged as SOLOs (1 transformation per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_60110-1_SDS-60110-HI-Control-10G-Chemically-Competent-Cells\"\u003eSDS 60110 HI Control 10G Chemically Competent Cells\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_60110-1_SDS0026-60110-1-HI-Control-10G-ChemComp\"\u003eSDS0026 60110 1 HI Control 10G ChemComp\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_60110-1_HI-Control\"\u003eHI Control\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"Default Title","offer_id":45986432516175,"sku":"60110-1","price":1179.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/HI-Control-10G-Chemically-Competent-Cells_02569925-6ebe-470f-bce0-9255c9cbd7b6.jpg?v=1785956129"},{"product_id":"e-cloni-10g-classic-electrocompetent-cells-efficiency-5-x-109-cfu-g-comcel-016","title":"E. cloni 10G Classic Electrocompetent Cells, Efficiency \u003e5 x 109 cfu\/µg","description":"\u003cp\u003eE. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5α\u0026amp;trade, DH10B\u0026amp;trade, JM109, TOP10, etc. and directly replace them in cloning protocols. However, E. cloni electrocompetent cells incorporate a unique manufacturing technology that increases transformation efficiency, recombinant yields and reliability (Figures. 1 and 2), while decreasing costs. These cells provide solutions for a wide range of applications at economical prices.\u003c\/p\u003e\u003cp\u003eChoice of strain:\u003cbr\u003eE. cloni 10G Competent Cells. Library construction, cloning, subcloning, and plasmid isolation with or without blue\/white screening.\u003cbr\u003eE. cloni 10GF′ Competent Cells. Contain the F' plasmid for infection with M13 to produce ssDNA.\u003cbr\u003eChoice of efficiency:\u003cbr\u003eE. cloni 10G SUPREME Electrocompetent Cells \u0026gt; 4 × 1010 cfu\/µg pUC DNA\u003cbr\u003eSUPREME Cells have the highest transformation efficiency available from any supplier. Choose SUPREME Cells for the most demanding cloning situations, such as construction of large, high complexity libraries or cloning difficult targets, which require the greatest number of transformants possible.\u003cbr\u003eE. cloni 10G \u0026amp; 10GF´ ELITE Electrocompetent Cells \u0026gt; 2 × 1010 cfu\/µg pUC DNA\u003cbr\u003eELITE Cells have twice the transformation efficiency compared to “ultra high efficiency” cells from other suppliers. ELITE Cells provide large numbers of transformants from hard-to-clone fragments or limited DNA at a lower price.\u003cbr\u003eE. cloni 10G CLASSIC Electrocompetent Cells \u0026gt; 5 × 109 cfu\/µg pUC DNA\u003cbr\u003eCLASSIC Cells are high efficiency cells with a substantially lower cost per reaction. These cells are the most economical choice for standard cloning and library construction. 10G CLASSIC Cells are available in larger package sizes for convenient use in higher volume cloning applications.\u003c\/p\u003e\u003cp\u003eCustom Competent Cells\u003c\/p\u003e\u003cp\u003eBulk cells or your strain of E. coli, made to the highest possible transfection efficiency with custom dispensing! See Custom Competent Cells page for detailed information.\u003c\/p\u003e\u003cp\u003ePlasmid cloning. E. cloni 10G SUPREME Cells provide recombinant yields higher than the highest efficiency cells offered by a leading supplier (Figures. 1 and 2)\u003c\/p\u003e\u003cp\u003eFigure 1. E. cloni 10G SUPREME Electrocompetent Cells consistently outperformed “ultra high efficiency” cells from a leading supplier. Both strains were transformed with 10 pg of pUC19 (n=16).\u003c\/p\u003e\u003cp\u003eFigure 2.Transformation efficiency comparison of E. cloni 10G SUPREME Cells and Mega DH10B cells (Invitrogen). Competent cells were transformed according to the manufacturer’s instructions with the same pUC19 DNA control (provided with the Invitrogen cells)\u003c\/p\u003e\u003cp\u003eTable 1. E. cloni Competent Cells for Cloning and Library Construction\u003c\/p\u003e\u003cp\u003eE. cloni Cell Lines\u003c\/p\u003e\u003cp\u003eTransformation Efficiency\u003cbr\u003e(cfu\/µg pUC DNA)\u003c\/p\u003e\u003cp\u003eCloning Methylated DNA\u003c\/p\u003e\u003cp\u003eBAC, Cosmid Cloning\u003c\/p\u003e\u003cp\u003eBlue\/White Screening\u003c\/p\u003e\u003cp\u003e10G SUPREME Electrocompetent\u003c\/p\u003e\u003cp\u003e\u0026gt;4 × 1010\u003c\/p\u003e\u003cp\u003eYES\u003c\/p\u003e\u003cp\u003eYES\u003c\/p\u003e\u003cp\u003eYES\u003cbr\u003ewithout IPTG induction\u003c\/p\u003e\u003cp\u003e10G ELITE Electrocompetent\u003c\/p\u003e\u003cp\u003e\u0026gt;2 × 1010\u003c\/p\u003e\u003cp\u003eYES\u003c\/p\u003e\u003cp\u003eNO\u003c\/p\u003e\u003cp\u003eYES\u003cbr\u003ewithout IPTG induction\u003c\/p\u003e\u003cp\u003e10G CLASSIC Electrocompetent\u003c\/p\u003e\u003cp\u003e\u0026gt;5 × 109\u003c\/p\u003e\u003cp\u003eYES\u003c\/p\u003e\u003cp\u003eNO\u003c\/p\u003e\u003cp\u003eYES\u003cbr\u003ewithout IPTG induction\u003c\/p\u003e\u003cp\u003e10GF´ ELITE Electrocompetent\u003c\/p\u003e\u003cp\u003e\u0026gt;2 × 1010\u003c\/p\u003e\u003cp\u003eYES\u003c\/p\u003e\u003cp\u003eNO\u003c\/p\u003e\u003cp\u003eYES\u003cbr\u003eIPTG induction required\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eDirect replacements for standard cloning strains\u003c\/li\u003e\n\u003cli\u003eOptimised genetics for high yields: phage T1 resistant, endonuclease and recombination minus, blue\/white screening-capable.\u003c\/li\u003e\n\u003cli\u003eAvailable in a range of transformation efficiencies .\u003c\/li\u003e\n\u003cli\u003eConvenient packaging options.\u003c\/li\u003e\n\u003cli\u003eHigh efficiency cells result in high colony counts\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eCompetent Cells include Control DNA and Recovery Medium, and are packaged as SOLOs (1 transformation per tube), DUOs (2 transformations per tube), Subcloning Grade (12 transformations per tube), or microplates as indicated. Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_COMCEL-016_60210-1-60210-2BAC-Optimized-Replicator\"\u003e60210 1 60210 2BAC Optimized Replicator\u003c\/a\u003e\u003c\/li\u003e\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"24 Reactions (SixPacks)","offer_id":45986432581711,"sku":"60117-1","price":1317.0,"currency_code":"ILS","in_stock":true},{"title":"48 Reactions (SixPacks)","offer_id":45986432614479,"sku":"60117-2","price":2345.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/E-cloni-10G-Classic-Electrocompetent-Cells_252C-Efficiency-_253E5-x-109-cfu-_25C2_25B5g_86a558c0-5a5a-4ddf-8ae2-849ce5ce6a21.jpg?v=1785956130"},{"product_id":"bac-optimised-electrocompetent-cells-60210","title":"BAC-Optimised Electrocompetent Cells","description":"\u003cp\u003eWe offer two bacterial strains designed for BAC library construction or transformation of large constructs:\u003c\/p\u003e\u003cp\u003eBAC-Optimised Replicator™ v2.0 Electrocompetent Cells (\u0026gt; 1 × 1010 cfu\/µg pKanR DNA). These cells are required for on-demand, copy number amplification of the pSMART® BAC or pEZ™ BAC vectors in the CopyRight® Kits, thereby increasing DNA yields. BAC-Optimised Replicator Cells are components of Biosearch Technologies Cloning Kits\u003c\/p\u003e\u003cp\u003eE. cloni 10G BAC-Optimised Electrocompetent Cells (\u0026gt; 1 × 107 cfu\/µg 150 kb BAC DNA; \u0026gt; 1 × 1010 cfu\/µg pUC DNA). The first competent cells developed specifically for large DNA cloning. Manufactured using a novel procedure that maximises transformation efficiencies with very large inserts, 10G BAC-Optimised Cells are an excellent choice for BAC library construction and large construct transformation.\u003c\/p\u003e\u003cp\u003eTable 1. E. cloni Competent Cells for BAC or Large Fragment Cloning\u003c\/p\u003e\u003cp\u003eE. cloni Cell Lines\u003c\/p\u003e\u003cp\u003eTransformation Efficiency\u003cbr\u003e(cfu\/µg pUC or pKanR DNA)\u003c\/p\u003e\u003cp\u003eCloning Methylated DNA\u003c\/p\u003e\u003cp\u003eBAC, Cosmid Cloning\u003c\/p\u003e\u003cp\u003eBlue\/White Screening\u003c\/p\u003e\u003cp\u003e10G BAC-Optimised Electrocompetent\u003c\/p\u003e\u003cp\u003e\u0026gt;1 × 1010\u003c\/p\u003e\u003cp\u003eYES\u003c\/p\u003e\u003cp\u003eYES\u003c\/p\u003e\u003cp\u003eYES\u003cbr\u003ewithout IPTG induction\u003c\/p\u003e\u003cp\u003eBAC-Optimised Replicator v2.0 Electrocompetent\u003c\/p\u003e\u003cp\u003e\u0026gt;1 × 1010\u003c\/p\u003e\u003cp\u003eYES\u003c\/p\u003e\u003cp\u003eYES\u003c\/p\u003e\u003cp\u003eYES\u003cbr\u003eIPTG induction required\u003c\/p\u003e\u003cp\u003eread more\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCells that enable the most challenging library construction and screening.\u003c\/li\u003e\n\u003cli\u003eHighest available transformation efficiencies for library construction and screening.\u003c\/li\u003e\n\u003cli\u003eOptimised for high yields of DNA.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cp\u003eReplicator v2.0: F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galUgalK rpsL nupG (attL araC-PBAD-trfA250 bla attR) λ-. Note that Replicator v2.0 cells are AmpR (bla gene) (StrR)\u003c\/p\u003e\u003cp\u003eE. cloni 10G BAC-Optimized: F - mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara, leu)7697 galU galK rpsL nupG λ- tonA (StrR)\u003c\/p\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eCompetent Cells include Control DNA and Recovery Medium, and are packaged as DUOs (2 transformations per tube) or FIVERs (5 transformations per tube), as indicated. Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_60210_60210-1-60210-2-BAC-Optimized-Replicator-v2-0-Electrocompetent-Cells\"\u003e60210 1 60210 2 BAC Optimized Replicator v2 0 Electrocompetent Cells\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/SDS_60210_sds-60210-1-60210-2-BAC-Optimized-Replicator-v2-0-Electrocompetent-Cells\"\u003esds 60210 1 60210 2 BAC Optimized Replicator v2 0 Electrocompetent Cells\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"12 Reactions (DUOs)","offer_id":45986432680015,"sku":"60210-1","price":1350.0,"currency_code":"ILS","in_stock":true},{"title":"24 Reactions (DUOs)","offer_id":45986432712783,"sku":"60210-2","price":2318.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/BAC-Optimised-Electrocompetent-Cells_0fe6ae4c-ebd4-4d86-8e83-3b1e17354d11.jpg?v=1785956134"},{"product_id":"endura-competent-cells-comcel-002","title":"Endura Competent Cells","description":"\u003cp\u003eMaintain unstable DNA.\u003cbr\u003eEndura Competent Cells are a commonly used strain for cloning sequences that suffer unwanted recombination events in other strains. Clones with inverted repeats or other sequences prone to recombination are commonly found in retroviral genes, and require cells such as Endura to be propagated stably.\u003c\/p\u003e\u003cp\u003eExcellent value and efficiency! Switch to Endura cells today and experience a higher level of efficiency and a savings in your cloning budget.\u003c\/p\u003e\u003cp\u003eChemical or Electrocompetent. Whichever method of transformation you prefer, we can give you better efficiency and\/or better prices.\u003c\/p\u003e\u003cp\u003eFigure 2. Transformation efficiencies of electrocompetent clone-stabilising strains.\u003c\/p\u003e\u003cp\u003eread more\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eClone repetitive sequences and lentiviral libraries\u003c\/li\u003e\n\u003cli\u003eStabilise direct repeats and create lentiviral constructs\u003c\/li\u003e\n\u003cli\u003eGenerate CRISPR-GeCKO-Libraries-Endura lentiviral guide RNA libraries\u003c\/li\u003e\n\u003cli\u003eRecommended in CRISPR GeCKO library protocols\u003c\/li\u003e\n\u003cli\u003eChoose electrocompetent or chemically competent cells\u003c\/li\u003e\n\u003cli\u003eHighest efficiency commercially available cells for lentiviral cloning\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cp\u003eGenotype:\u003c\/p\u003e\u003cp\u003erecA13 supE44 ara-14 galK2 lacY1 proA2 rpsL20(StrR) xyl-5 λ– leu mtl-1 F– mcrB mrr hsdS20(rB–, mB–)\u003c\/p\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eEndura™ Competent Cells include Control DNA and Recovery Medium, and are packaged as SOLOs (1 transformation per tube) or DUOs (2 transformations per tube) as indicated. Endura Electrocompetent cells are provided in DUOs format only. Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_COMCEL-002_60240-Endura-Chemically-Competent-Cells-Duo\"\u003e60240 Endura Chemically Competent Cells Duo\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_COMCEL-002_60241-Endura-Chemically-Competent-Cells-Solo\"\u003e60241 Endura Chemically Competent Cells Solo\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_COMCEL-002_60242-Endura-ElectroCompetent-Cells\"\u003e60242 Endura ElectroCompetent Cells\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_COMCEL-002_Endura-Competent-Cells\"\u003eEndura Competent Cells\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/pis_COMCEL-002_F301-US-Endura-Comp-Cells\"\u003eF301 US Endura Comp Cells\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"12 Reactions (DUOs)","offer_id":45986432745551,"sku":"60240-1","price":1085.0,"currency_code":"ILS","in_stock":true},{"title":"24 Reactions (DUOs)","offer_id":45986432778319,"sku":"60240-2","price":1910.0,"currency_code":"ILS","in_stock":true},{"title":"12 Reactions (SOLOs)","offer_id":45986432811087,"sku":"60241-1","price":1193.0,"currency_code":"ILS","in_stock":true},{"title":"12 Reactions (DUOs) (60242-1)","offer_id":45986432843855,"sku":"60242-1","price":1273.0,"currency_code":"ILS","in_stock":true},{"title":"24 Reactions (DUOs) (60242-2)","offer_id":45986432876623,"sku":"60242-2","price":2231.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/Endura-Competent-Cells_bee79760-e2b5-4e13-a306-14feb5a19bdc.jpg?v=1785956135"},{"product_id":"e-cloni-express-competent-cells-comcel-003","title":"E. cloni EXPRESS Competent Cells","description":"\u003cp\u003eE. cloni EXPRESS BL21(DE3) Electrocompetent Cells are the first to offer high efficiency cloning and high level protein expression in the same cell. Cloning efficiencies are increased 25-1,000 fold relative to competitors’ preparations of BL21 cells (Figure 1), which is essential for construction of complex expression libraries. The unprecedented transformation efficiency of E. cloni EXPRESS Electrocompetent Cells (\u0026gt; 5 × 109 cfu\/µg) eliminates the need for plasmid transfer from the cloning strain to the expression strain, saving days of work in a typical cloning and expression experiment (Figure. 2).\u003c\/p\u003e\u003cp\u003eFigure 1. Transformation efficiency comparison of commercially available BL21(DE3) competent cells.\u003c\/p\u003e\u003cp\u003eFigure 2. Comparison of cloning and protein expression procedures.\u003c\/p\u003e\u003cp\u003eE. cloni EXPRESS BL21(DE3) strains contain the phage T7 RNA polymerase gene linked to the IPTG-inducible promoter, for use with any expression plasmid containing the T7 promoter.\u003c\/p\u003e\u003cp\u003eNOTE: For best results in expressing proteins that may be toxic, we recommend using OverExpress Cells. E. cloni EXPRESS cells are also available as chemically competent cells. With a transformation efficiency of \u0026gt;1 × 107 cfu\/µg and very economical prices, EXPRESS Chemically Competent Cells offer superior value for everyday protein expression work.\u003c\/p\u003e\u003cp\u003eread more\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eBest value for routine protein expression\u003c\/li\u003e\n\u003cli\u003eAvailable as Electrocompetent and Chemically Competent Cells.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cp\u003eGenotype\u003c\/p\u003e\u003cp\u003eE. cloni EXPRESS BL21(DE3): F– ompT hsdSB (rB- mB-) gal dcm lon λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5])\u003c\/p\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eEach E. cloni EXPRESS Kit contains: the indicated E. cloni EXPRESS Electrocompetent or Chemically Competent Cells in DUO packaging (2 transformations per tube), Expression Recovery Medium (lactose minus), pUC19 Positive Control Plasmid, and complete protocols.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_COMCEL-003_60300-E.-cloni-EXPRESS-BL21DE3-Electrocompetent-Cells\"\u003e60300 E. cloni EXPRESS BL21DE3 Electrocompetent Cells\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_COMCEL-003_60401-E.-cloni-EXPRESS-BL21DE3-Chemically-Competent-Cells\"\u003e60401 E. cloni EXPRESS BL21DE3 Chemically Competent Cells\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_COMCEL-003_Ecloni%20EXPRESS%20Chemically%20Competent%20Cells%20v2.4\"\u003eEcloni EXPRESS Chemically Competent Cells v2.4\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_COMCEL-003_Ecloni-EXPRESS-Electrocompetent-Cells\"\u003eEcloni EXPRESS Electrocompetent Cells\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"12 Rxns (DUOs)","offer_id":45986432974927,"sku":"60300-1","price":848.0,"currency_code":"ILS","in_stock":true},{"title":"24 Rxns (DUOs)","offer_id":45986433007695,"sku":"60300-2","price":1514.0,"currency_code":"ILS","in_stock":true},{"title":"12 Rxns (DUOs) (60401-1)","offer_id":45986433040463,"sku":"60401-1","price":523.0,"currency_code":"ILS","in_stock":true},{"title":"24 Reactions (DUOs)","offer_id":45986433073231,"sku":"60401-2","price":744.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/E-cloni-EXPRESS-Competent-Cells_84c44f79-ec05-4583-8d12-1cb8f1b095eb.jpg?v=1785956139"},{"product_id":"overexpress-electrocompetent-cells-comcel-015","title":"OverExpress Electrocompetent Cells","description":"\u003cp\u003eE. coli BL21(DE3) strains, like E. cloni™ EXPRESS Competent Cells provide reliable expression of many genes cloned into T7 expression vectors (e.g.., pET or pSMART™-cDNA vectors). However, in some cases expression is minimal or not detectable because the recombinant protein, when expressed, is deleterious or lethal to these standard BL21 strains. Examples of such toxic proteins include many membrane proteins, some cytoplasmic proteins, and nucleases. Unfortunately, successful expression of one or more toxic proteins is often important to the experimental goal.\u003c\/p\u003e\u003cp\u003eOverExpress Electrocompetent and Chemically Competent Cells are E. coli strains that are effective in expressing toxic proteins from all classes of organisms, including eubacteria, yeasts, plants, viruses, and mammals. The effectiveness of these new strains in expressing toxic proteins has been validated in more than 350 publications.\u003c\/p\u003e\u003cp\u003eThe OverExpress strains contain genetic mutations phenotypically selected for conferring tolerance to toxic proteins. The strain C41(DE3) was derived from BL21(DE3). This strain has at least one mutation, which prevents cell death associated with expression of many recombinant toxic proteins. The strain C43(DE3) was derived from C41(DE3) by selecting for resistance to a different toxic protein and can express a different set of toxic proteins to C41(DE3). Figure. 1 graphically illustrates the advantages of the OverExpress Competent Cells, compared to standard BL21(DE3) cells, in expressing toxic proteins.\u003c\/p\u003e\u003cp\u003eFigure 1. Green Fluorescent Protein (top) or Red Fluorescent Protein (bottom) expressed from a T7 promoter construct that was transformed into C41, BL21, or C43 competent cells spread on IPTG plates to induce protein expression.\u003c\/p\u003e\u003cp\u003eTable. 1 and Figure. 2 summarise transformation effectiveness, tolerance of expression-induced toxicity, and protein expression for T7 expression plasmids coding for a variety of recombinant proteins. These results demonstrate that the OverExpress C41(DE3) and C43(DE3) strains are clearly superior to the parental BL21(DE3) in transformation and expression of toxic proteins.\u003c\/p\u003e\u003cp\u003eTable 1. Comparison of OverExpress C41(DE3) and C43(DE3) cells with the parental strain BL21(DE3) in transformation and expression of heterologous proteins.**\u003c\/p\u003e\u003cp\u003eStrain\t\u003cbr\u003eTransformation\u003cbr\u003eSuccess Ratea\u003c\/p\u003e\u003cp\u003eExpression-induced Toxicityb\u003c\/p\u003e\u003cp\u003eExpressing Plasmidsc\u003c\/p\u003e\u003cp\u003eBL21(DE3)\u003c\/p\u003e\u003cp\u003e16\/26 (62%)\u003c\/p\u003e\u003cp\u003e25\/26 (96%)\u003c\/p\u003e\u003cp\u003e14\/26 (54%)\u003c\/p\u003e\u003cp\u003eC41(DE3)\u003c\/p\u003e\u003cp\u003e28\/28 (100%)\u003c\/p\u003e\u003cp\u003e14\/28 (50%)\u003c\/p\u003e\u003cp\u003e24\/28 (86%)\u003c\/p\u003e\u003cp\u003eC43(DE3)\u003c\/p\u003e\u003cp\u003e28\/28 (100%)\u003c\/p\u003e\u003cp\u003e1\/28 (4%)\u003c\/p\u003e\u003cp\u003e23\/28 (81%)\u003c\/p\u003e\u003cp\u003eFigure 2. Comparison of OverExpress C41(DE3) and C43(DE3) cells with the parental strain BL21(DE3) in transformation and expression of heterologous proteins.**\u003c\/p\u003e\u003cp\u003ea Transformation success corresponds to the presence of colonies on LB+ampicillin agar following transformation with a plasmid.\u003cbr\u003eb Expression toxicity corresponds to the absence of colonies on LB+ampicillin+IPTG agar following transformation with a plasmid.\u003cbr\u003ec Expressing plasmids corresponds to observation of a heterologous protein in the total cell pellet on Coomassie-stained SDS-PAGE following growth of a colony in LB+ampicillin medium and induction with IPTG.\u003cbr\u003e**L. Dumon-Seignovert, G. Cariot, and L. Vuillard (2004). Protein Expression and Purification 37, 203-206. Data used with permission.\u003c\/p\u003e\u003cp\u003eAs in standard BL21(DE3) strains, OverExpress C41(DE3), C41(DE3)pLysS, C43(DE3), and C43 (DE3)pLysS are lysogens of λDE3. These strains carry a chromosomal copy of the T7 RNA Polymerase gene under the control of the lacUV5 promoter. These strains are suitable for production of protein from target genes cloned into T7-driven expression vectors. OverExpress C41(DE3), C41(DE3) pLysS, C43(DE3), and C43(DE3) pLysS are also deficient in the lon and ompT proteases.\u003c\/p\u003e\u003cp\u003eOverExpress C41(DE3)pLysS and C43(DE3)pLysS also carry a chloramphenicol-resistant plasmid that encodes T7 lysozyme, which is a natural inhibitor of T7 RNA polymerase. Cells containing pLysS produce a small amount of T7 lysozyme. These strains are used to suppress basal expression of T7 RNA polymerase prior to induction, thus stabilising recombinants encoding particularly toxic proteins.\u003c\/p\u003e\u003cp\u003eread more\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eExpress genes cloned into any T7 vector with these BL21(DE3) derivatives\u003c\/li\u003e\n\u003cli\u003eEffective in expressing toxic membrane proteins\u003c\/li\u003e\n\u003cli\u003eCited in over 350 research articles\u003c\/li\u003e\n\u003cli\u003eRecommended for membrane proteins with T7 vector\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cp\u003eOverExpress Genotypes\u003cbr\u003eOverExpress C41(DE3): F – ompT hsdSB (rB- mB-) gal dcm (DE3)\u003cbr\u003eOverExpress C41(DE3)pLysS: F – ompT hsdSB (rB- mB-) gal dcm (DE3) pLysS (CmR)\u003cbr\u003eOverExpress C43(DE3): F – ompT hsdSB (rB- mB-) gal dcm (DE3)\u003cbr\u003eOverExpress C43(DE3)pLysS: F – ompT hsdSB (rB- mB-) gal dcm (DE3) pLysS (CmR)\u003c\/p\u003e\u003cp\u003eTable 1. OverExpress Transformation Efficiencies\u003c\/p\u003e\u003cp\u003eElectrocompetent Cells\t\u003cbr\u003ecfu\/µg DNA\u003c\/p\u003e\u003cp\u003eC41(DE3)\t\u003cbr\u003e\u0026gt; 1 × 109\u003c\/p\u003e\u003cp\u003eC43(DE3)\t\u003cbr\u003e\u0026gt; 1 × 109\u003c\/p\u003e\u003cp\u003eChemically Competent Cells\u003cbr\u003eC41(DE3)\t\u003cbr\u003e\u0026gt; 1 × 106\u003c\/p\u003e\u003cp\u003eC41(DE3)pLysS\t\u003cbr\u003e\u0026gt; 1 × 106\u003c\/p\u003e\u003cp\u003eC43(DE3)\t\u003cbr\u003e\u0026gt; 1 × 106\u003c\/p\u003e\u003cp\u003eC43(DE3)pLysS\t\u003cbr\u003e\u0026gt; 1 × 106\u003cbr\u003eread more\u003c\/p\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eEach OverExpress kit contains Electrocompetent Cells in SOLO packaging (1 transformation per tube), Expression Recovery Medium (lactose minus), pUC19 Positive Control Plasmid, pAVD10 Verification Plasmid, and complete protocols.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_COMCEL-015_60341-OverExpress-C41-DE3-Electrocompetent-Cells\"\u003e60341 OverExpress C41 DE3 Electrocompetent Cells\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_COMCEL-015_60345-OverExpress-C43-DE3-Electrocompetent-Cells\"\u003e60345 OverExpress C43 DE3 Electrocompetent Cells\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_COMCEL-015_OverExpress-Chemicallycompetent-Cells\"\u003eOverExpress Chemicallycompetent Cells\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_COMCEL-015_OverExpress-Electrocompetent-Cells\"\u003eOverExpress Electrocompetent Cells\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/pis_COMCEL-015_F304-OverExpress-US\"\u003eF304 OverExpress US\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"12 Reactions (SOLOs)","offer_id":45986433105999,"sku":"60341-1","price":2057.0,"currency_code":"ILS","in_stock":true},{"title":"12 Reactions (SOLOs) (60345-1)","offer_id":45986433138767,"sku":"60345-1","price":2057.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/OverExpress-Electrocompetent-Cells_db42e32c-d52c-4709-b86a-a9bbd167bd3e.jpg?v=1785956139"},{"product_id":"hi-control-bl21-de3-chemically-competent-cells-60435-1","title":"HI-Control BL21(DE3) Chemically Competent Cells","description":"\u003cp\u003eThe HI-Control™ strains are based on E. cloni 10G ( DH10B) and BL21(DE3) strains. These strains harbour a plasmid bearing a constitutive lacI repressor gene, which provides tight control over promoters containing the lacO operator.\u003c\/p\u003e\u003cp\u003eTightly Control Protein Expression\t \t \u003cbr\u003e \tT7 Promoters\tNon-T7 lac promoters\u003cbr\u003e(T5-lac, tac, trc, lac)\tClone with low background target expression\tTransformation efficiencies\u003cbr\u003eHI-Control\u003cbr\u003eBL21(DE3)\t \t \t \t≥ 1×107 cfu\/µg\u003cbr\u003eHI-Control\u003cbr\u003e10G\t \t \t \t≥ 1×109 cfu\/µg\u003cbr\u003eGenotypes:\u003c\/p\u003e\u003cp\u003eHI-Control BL21 (DE3): F- ompT hsdSB (rB- mB-) gal dcm (DE3) Mini-F lacIq1(GentR)\u003c\/p\u003e\u003cp\u003eHI-Control 10G: mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 ɸ80dlacZΔM15 ΔlacX74 araD139 Δ (ara,leu)7697 galU galK rpsL (StrR) nupG λ− tonA Mini-F lacIq1 (GentR)\u003c\/p\u003e\u003cp\u003eread more\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eReduce leaky expression: The LacI repressor protein binds to the lacO operator, blocking transcription of downstream genes. In the absence of an induction agent such as lactose sugar or IPTG, target protein expression from the adjacent promoter is greatly reduced.\u003c\/li\u003e\n\u003cli\u003eObtain high protein yields:Both 10G and BL21(DE3)-based strains express high levels of protein. For expression from a T7 promoter, select the HI-Control BL21(DE3) strain. Other lac-based promoters like T5-lac, tac, trc, and lac, can be used in either HI-Control strain.\u003c\/li\u003e\n\u003cli\u003eAchieve high efficiency transformations: Highly efficient competent cells give excellent results from small amounts of plasmid DNA.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cp\u003ePlease note that the SUMO Protease and SUMO Fusion tag are modified from the original native proteins. Native or non-modified control proteins or proteases will not be compatible.\u003c\/p\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eCompetent Cells include Control DNA and Recovery Medium, and are packaged as SOLOs (1 transformation per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_60435-1_SDS-60435-HI-Control-BL21DE3-Chemically-Competent-Cells\"\u003eSDS 60435 HI Control BL21DE3 Chemically Competent Cells\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_60435-1_SDS0025-60435-1-HI-Control-BL21-DE3-ChemComp\"\u003eSDS0025 60435 1 HI Control BL21 DE3 ChemComp\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_60435-1_HI-Control\"\u003eHI Control\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"Default Title","offer_id":45986433400911,"sku":"60435-1","price":1179.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/HI-Control-BL21_2528DE3_2529-Chemically-Competent-Cells_19a2333c-55d0-484c-b49e-1c365a2f061c.jpg?v=1785956144"},{"product_id":"overexpress-chemically-competent-cells-comcel-004","title":"OverExpress Chemically Competent Cells","description":"\u003cp\u003eE. coli BL21(DE3) strains, like E. cloni™ EXPRESS Competent Cells provide reliable expression of many genes cloned into T7 expression vectors (e.g.., pET or pSMART™-cDNA vectors). However, in some cases expression is minimal or not detectable because the recombinant protein, when expressed, is deleterious or lethal to these standard BL21 strains. Examples of such toxic proteins include many membrane proteins, some cytoplasmic proteins, and nucleases. Unfortunately, successful expression of one or more toxic proteins is often important to the experimental goal.\u003c\/p\u003e\u003cp\u003eOverExpress Electrocompetent and Chemically Competent Cells are E. coli strains that are effective in expressing toxic proteins from all classes of organisms, including eubacteria, yeasts, plants, viruses, and mammals. The effectiveness of these new strains in expressing toxic proteins has been validated in more than 350 publications.\u003c\/p\u003e\u003cp\u003eThe OverExpress strains contain genetic mutations phenotypically selected for conferring tolerance to toxic proteins. The strain C41(DE3) was derived from BL21(DE3). This strain has at least one mutation, which prevents cell death associated with expression of many recombinant toxic proteins. The strain C43(DE3) was derived from C41(DE3) by selecting for resistance to a different toxic protein and can express a different set of toxic proteins to C41(DE3). Figure. 1 graphically illustrates the advantages of the OverExpress Competent Cells, compared to standard BL21(DE3) cells, in expressing toxic proteins.\u003c\/p\u003e\u003cp\u003eFigure 1. Green Fluorescent Protein (top) or Red Fluorescent Protein (bottom) expressed from a T7 promoter construct that was transformed into C41, BL21, or C43 competent cells spread on IPTG plates to induce protein expression.\u003c\/p\u003e\u003cp\u003eTable. 1 and Figure. 2 summarise transformation effectiveness, tolerance of expression-induced toxicity, and protein expression for T7 expression plasmids coding for a variety of recombinant proteins. These results demonstrate that the OverExpress C41(DE3) and C43(DE3) strains are clearly superior to the parental BL21(DE3) in transformation and expression of toxic proteins.\u003c\/p\u003e\u003cp\u003eTable 1. Comparison of OverExpress C41(DE3) and C43(DE3) cells with the parental strain BL21(DE3) in transformation and expression of heterologous proteins.**\u003c\/p\u003e\u003cp\u003eStrain\t\u003cbr\u003eTransformation\u003cbr\u003eSuccess Ratea\u003c\/p\u003e\u003cp\u003eExpression-induced Toxicityb\u003c\/p\u003e\u003cp\u003eExpressing Plasmidsc\u003c\/p\u003e\u003cp\u003eBL21(DE3)\u003c\/p\u003e\u003cp\u003e16\/26 (62%)\u003c\/p\u003e\u003cp\u003e25\/26 (96%)\u003c\/p\u003e\u003cp\u003e14\/26 (54%)\u003c\/p\u003e\u003cp\u003eC41(DE3)\u003c\/p\u003e\u003cp\u003e28\/28 (100%)\u003c\/p\u003e\u003cp\u003e14\/28 (50%)\u003c\/p\u003e\u003cp\u003e24\/28 (86%)\u003c\/p\u003e\u003cp\u003eC43(DE3)\u003c\/p\u003e\u003cp\u003e28\/28 (100%)\u003c\/p\u003e\u003cp\u003e1\/28 (4%)\u003c\/p\u003e\u003cp\u003e23\/28 (81%)\u003c\/p\u003e\u003cp\u003eFigure 2. Comparison of OverExpress C41(DE3) and C43(DE3) cells with the parental strain BL21(DE3) in transformation and expression of heterologous proteins.**\u003c\/p\u003e\u003cp\u003ea Transformation success corresponds to the presence of colonies on LB+ampicillin agar following transformation with a plasmid.\u003cbr\u003eb Expression toxicity corresponds to the absence of colonies on LB+ampicillin+IPTG agar following transformation with a plasmid.\u003cbr\u003ec Expressing plasmids corresponds to observation of a heterologous protein in the total cell pellet on Coomassie-stained SDS-PAGE following growth of a colony in LB+ampicillin medium and induction with IPTG.\u003cbr\u003e**L. Dumon-Seignovert, G. Cariot, and L. Vuillard (2004). Protein Expression and Purification 37, 203-206. Data used with permission.\u003c\/p\u003e\u003cp\u003eAs in standard BL21(DE3) strains, OverExpress C41(DE3), C41(DE3)pLysS, C43(DE3), and C43 (DE3)pLysS are lysogens of λDE3. These strains carry a chromosomal copy of the T7 RNA Polymerase gene under the control of the lacUV5 promoter. These strains are suitable for production of protein from target genes cloned into T7-driven expression vectors. OverExpress C41(DE3), C41(DE3) pLysS, C43(DE3), and C43(DE3) pLysS are also deficient in the lon and ompT proteases.\u003c\/p\u003e\u003cp\u003eOverExpress C41(DE3)pLysS and C43(DE3)pLysS also carry a chloramphenicol-resistant plasmid that encodes T7 lysozyme, which is a natural inhibitor of T7 RNA polymerase. Cells containing pLysS produce a small amount of T7 lysozyme. These strains are used to suppress basal expression of T7 RNA polymerase prior to induction, thus stabilising recombinants encoding particularly toxic proteins.\u003c\/p\u003e\u003cp\u003eWhich OverExpress cell strain should I use?\u003c\/p\u003e\u003cp\u003eIt is difficult to predict which of the four OverExpress strains – C41(DE3), C43(DE3), C41(DE3)pLysS, or C43(DE3)pLysS – will work best in expressing a given protein. We recommend initially using the OverExpress ComboPack™ which contains 3 reactions each of the four OverExpress competent cell strains, to determine which one is best for your application. The OverExpress strains are available as electrocompetent or chemically competent cells.\u003c\/p\u003e\u003cp\u003eBecause there are no intrinsic antibiotic resistances (or plasmids) in either C41(DE3) or C43(DE3), the strains can be differentiated from each other and from BL21(DE3) by transformation with a strain verification vector, pAVD10. pAVD10 contains the uncF gene (encoding the beta-subunit of E. coli ATPase) under the control of the T7 promoter. This plasmid is lethal to BL21(DE3) and to induced C41(DE3), but it is tolerated by C43(DE3) regardless of induction. pAVD10 is provided with OverExpress Cells.\u003c\/p\u003e\u003cp\u003eRead more\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eExpress genes cloned into any T7 vector with these BL21(DE3) derivatives\u003c\/li\u003e\n\u003cli\u003eEffective in expressing toxic membrane proteins\u003c\/li\u003e\n\u003cli\u003eCited in over 350 research articles\u003c\/li\u003e\n\u003cli\u003eRecommended for membrane proteins with T7 vector\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cp\u003eOverExpress Genotypes\u003cbr\u003eOverExpress C41(DE3): F – ompT hsdSB (rB- mB-) gal dcm (DE3)\u003cbr\u003eOverExpress C41(DE3)pLysS: F – ompT hsdSB (rB- mB-) gal dcm (DE3) pLysS (CmR)\u003cbr\u003eOverExpress C43(DE3): F – ompT hsdSB (rB- mB-) gal dcm (DE3)\u003cbr\u003eOverExpress C43(DE3)pLysS: F – ompT hsdSB (rB- mB-) gal dcm (DE3) pLysS (CmR)\u003c\/p\u003e\u003cp\u003eTable 1. OverExpress Transformation Efficiencies\u003c\/p\u003e\u003cp\u003eElectrocompetent Cells\t\u003cbr\u003ecfu\/µg DNA\u003c\/p\u003e\u003cp\u003eC41(DE3)\t\u003cbr\u003e\u0026gt; 1 × 109\u003c\/p\u003e\u003cp\u003eC43(DE3)\t\u003cbr\u003e\u0026gt; 1 × 109\u003c\/p\u003e\u003cp\u003eChemically Competent Cells\u003cbr\u003eC41(DE3)\t\u003cbr\u003e\u0026gt; 1 × 106\u003c\/p\u003e\u003cp\u003eC41(DE3)pLysS\t\u003cbr\u003e\u0026gt; 1 × 106\u003c\/p\u003e\u003cp\u003eC43(DE3)\t\u003cbr\u003e\u0026gt; 1 × 106\u003c\/p\u003e\u003cp\u003eC43(DE3)pLysS\t\u003cbr\u003e\u0026gt; 1 × 106\u003cbr\u003eRead more\u003c\/p\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eEach OverExpress kit contains Chemically Competent Cells in SOLO packaging (1 transformation per tube), Expression Recovery Medium (lactose minus), pUC19 Positive Control Plasmid, pAVD10 Verification Plasmid, and complete protocols. ComboPacks contain 3 reactions each of chemically competent C41(DE3), C43(DE3), C41(DE)pLysS, and C43(DE3)pLysS.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_COMCEL-004_60442-OverExpress-C41-DE3-Chemically-Competent-Cells\"\u003e60442 OverExpress C41 DE3 Chemically Competent Cells\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_COMCEL-004_60444-OverExpress-C41-DE3-pLysS-Chemically-Competent-Cells\"\u003e60444 OverExpress C41 DE3 pLysS Chemically Competent Cells\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_COMCEL-004_60446-OverExpress-C43-DE3-Chemically-Competent-Cells\"\u003e60446 OverExpress C43 DE3 Chemically Competent Cells\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_COMCEL-004_60448-OverExpress-C43-DE3-pLysS-Chemically-Competent-Cells\"\u003e60448 OverExpress C43 DE3 pLysS Chemically Competent Cells\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_COMCEL-004_60452-OverExpress-ChemCombo-Pack\"\u003e60452 OverExpress ChemCombo Pack\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_COMCEL-004_OverExpress-Chemicallycompetent-Cells\"\u003eOverExpress Chemicallycompetent Cells\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_COMCEL-004_OverExpress-Electrocompetent-Cells\"\u003eOverExpress Electrocompetent Cells\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/pis_COMCEL-004_F304-OverExpress-US\"\u003eF304 OverExpress US\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"12 Reactions (SOLOs)","offer_id":45986433237071,"sku":"60442-1","price":1702.0,"currency_code":"ILS","in_stock":true},{"title":"12 Reactions (SOLOs) (60444-1)","offer_id":45986433269839,"sku":"60444-1","price":1705.0,"currency_code":"ILS","in_stock":true},{"title":"12 Reactions (SOLOs) (60446-1)","offer_id":45986433302607,"sku":"60446-1","price":1702.0,"currency_code":"ILS","in_stock":true},{"title":"12 Reactions (SOLOs) (60448-1)","offer_id":45986433335375,"sku":"60448-1","price":1705.0,"currency_code":"ILS","in_stock":true},{"title":"12 Reactions (SOLOs) (60452-1)","offer_id":45986433368143,"sku":"60452-1","price":1702.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/OverExpress-Chemically-Competent-Cells_dd4e0682-a5a1-4d77-9e02-3f047c232263.jpg?v=1785956144"},{"product_id":"tg1-electrocompetent-cells-comcel-014","title":"TG1 Electrocompetent Cells","description":"\u003cp\u003eTG1 Electrocompetent Cells. An amber suppressor strain (supE) prepared as highly efficient (≥4 × 1010 cfu\/µg) electrocompetent cells for phage display library screening. These cells are used for phage display and protein expression\u003c\/p\u003e\u003cp\u003eFigure 1. Transformation efficiency of electrocompetent bacterial cells for phage display compared to competitor's specification.\u003c\/p\u003e\u003cp\u003eTable 1. Competent Cells for Phage Display libraries\u003c\/p\u003e\u003cp\u003eCell Lines\u003c\/p\u003e\u003cp\u003eTransformation Efficiency\u003cbr\u003e(cfu\/µg pUC DNA)\u003c\/p\u003e\u003cp\u003eCloning Methylated DNA\u003c\/p\u003e\u003cp\u003eBAC, Cosmid Cloning\u003c\/p\u003e\u003cp\u003eBlue\/White Screening\u003c\/p\u003e\u003cp\u003eTG1 Electrocompetent\u003c\/p\u003e\u003cp\u003e\u0026gt;4 × 1010\u003c\/p\u003e\u003cp\u003eYES\u003c\/p\u003e\u003cp\u003eNO\u003c\/p\u003e\u003cp\u003eYES\u003cbr\u003eIPTG induction required\u003c\/p\u003e\u003cp\u003eSS320 (MC1061 F') Electrocompetent\u003c\/p\u003e\u003cp\u003e\u0026gt;4× 1010\u003c\/p\u003e\u003cp\u003eYES\u003c\/p\u003e\u003cp\u003eNO\u003c\/p\u003e\u003cp\u003eYES\u003cbr\u003eIPTG induction required\u003c\/p\u003e\u003cp\u003eMC1061 F-Electrocompetent\u003c\/p\u003e\u003cp\u003e\u0026gt;4× 1010\u003c\/p\u003e\u003cp\u003eYES\u003c\/p\u003e\u003cp\u003eNO\u003c\/p\u003e\u003cp\u003eYES\u003cbr\u003eIPTG induction required\u003c\/p\u003e\u003cp\u003eER2738 Electrocompetent\u003c\/p\u003e\u003cp\u003e\u0026gt;2 × 1010\u003c\/p\u003e\u003cp\u003eYES\u003c\/p\u003e\u003cp\u003eNO\u003c\/p\u003e\u003cp\u003eYES\u003cbr\u003eIPTG induction required\u003c\/p\u003e\u003cp\u003eread more\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003ePerfect for antibody phage display library creation\u003c\/li\u003e\n\u003cli\u003eThe highest efficiency TG1 competent cells available.\u003c\/li\u003e\n\u003cli\u003eCreate larger libraries; speed discovery\u003c\/li\u003e\n\u003cli\u003eBulk custom dispensing available with all of our phage display cells\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cp\u003eGenotype\u003c\/p\u003e\u003cp\u003eTG1: [F' traD36 proAB lacIqZ ΔM15] supE thi-1 Δ(lac-proAB) Δ(mcrB-hsdSM)5(rK - mK -)\u003cbr\u003eSS320 (MC1061F'): F'[proAB lacIqZ ΔM15 Tn10 (TetR)] araD139 Δ(ara-leu)7696 galE15 galK16 Δ(lac)X74 rpsL (StrR) hsdR2 (rK– mK+) mcrA mcrB1\u003cbr\u003eER2738: [F'proA+B+ lacIq Δ(lacZ)M15 zzf::Tn10 (tetr)] fhuA2 glnVΔ(lac-proAB) thi-1Δ(hsdS-mcrB)5\u003cbr\u003eMC1061 F-: F-, araD139 Δ(ara-leu)7696 galE15 galK16 Δ(lac)X74 rpsL (StrR) hsdR2 (rK– mK+) mcrA mcrB1\u003c\/p\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eCompetent Cells include Control DNA and Recovery Medium and are packaged as DUOs (2 transformations per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.\u003c\/p\u003e\n\u003ch2\u003eResources\u003c\/h2\u003e\n\u003cp\u003eApplication notes\u003c\/p\u003e\u003cp\u003etechnical note E.coli strains for phage display\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_COMCEL-014_60502-1-60502-2-TG1-Electrocompetent-Cells\"\u003e60502 1 60502 2 TG1 Electrocompetent Cells\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_COMCEL-014_MC1061F-Electro\"\u003eMC1061F Electro\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_COMCEL-014_Phage-Display\"\u003ePhage Display\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/pis_COMCEL-014_F302-US-phage-display-comp-cells\"\u003eF302 US phage display comp cells\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/app-note_COMCEL-014_technical-note-E.coli-strains-for-phage-display\"\u003etechnical note E.coli strains for phage display\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"12 Reactions (DUOs)","offer_id":45986433499215,"sku":"60502-1","price":1317.0,"currency_code":"ILS","in_stock":true},{"title":"24 Reactions (DUOs)","offer_id":45986433531983,"sku":"60502-2","price":2278.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TG1-Electrocompetent-Cells_beb4a083-a68d-4798-bc02-223062c0c9ae.jpg?v=1785956148"},{"product_id":"ss320-mc1061-f-electrocompetent-cells-comcel-013","title":"SS320 (MC1061 F') Electrocompetent Cells","description":"\u003cp\u003eSS320 Electrocompetent Cells. A non-amber suppressor strain (sometimes called MC1061F') prepared as highly efficient (≥4 × 1010 cfu\/µg) electrocompetent cells for phage display library screening. These cells have the highest available transformation efficiency of any strain used for phage display.\u003c\/p\u003e\u003cp\u003eFigure 1. Transformation efficiency of electrocompetent bacterial cells for phage display compared to competitor's specification.\u003c\/p\u003e\u003cp\u003eTable 1. Competent Cells for Phage Display libraries\u003c\/p\u003e\u003cp\u003eCell Lines\u003c\/p\u003e\u003cp\u003eTransformation Efficiency\u003cbr\u003e(cfu\/µg pUC DNA)\u003c\/p\u003e\u003cp\u003eCloning Methylated DNA\u003c\/p\u003e\u003cp\u003eBAC, Cosmid Cloning\u003c\/p\u003e\u003cp\u003eBlue\/White Screening\u003c\/p\u003e\u003cp\u003eTG1 Electrocompetent\u003c\/p\u003e\u003cp\u003e\u0026gt;4 × 1010\u003c\/p\u003e\u003cp\u003eYES\u003c\/p\u003e\u003cp\u003eNO\u003c\/p\u003e\u003cp\u003eYES\u003cbr\u003eIPTG induction required\u003c\/p\u003e\u003cp\u003eSS320 (MC1061 F') Electrocompetent\u003c\/p\u003e\u003cp\u003e\u0026gt;4× 1010\u003c\/p\u003e\u003cp\u003eYES\u003c\/p\u003e\u003cp\u003eNO\u003c\/p\u003e\u003cp\u003eYES\u003cbr\u003eIPTG induction required\u003c\/p\u003e\u003cp\u003eMC1061 F-Electrocompetent\u003c\/p\u003e\u003cp\u003e\u0026gt;4× 1010\u003c\/p\u003e\u003cp\u003eYES\u003c\/p\u003e\u003cp\u003eNO\u003c\/p\u003e\u003cp\u003eYES\u003cbr\u003eIPTG induction required\u003c\/p\u003e\u003cp\u003eER2738 Electrocompetent\u003c\/p\u003e\u003cp\u003e\u0026gt;2 × 1010\u003c\/p\u003e\u003cp\u003eYES\u003c\/p\u003e\u003cp\u003eNO\u003c\/p\u003e\u003cp\u003eYES\u003cbr\u003eIPTG induction required\u003c\/p\u003e\u003cp\u003eread more\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003ePerfect for antibody phage display library creation\u003c\/li\u003e\n\u003cli\u003eCreate larger libraries; speed discovery\u003c\/li\u003e\n\u003cli\u003eBulk custom dispensing available with all of our phage display cells\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cp\u003eGenotype\u003c\/p\u003e\u003cp\u003eTG1: [F' traD36 proAB lacIqZ ΔM15] supE thi-1 Δ(lac-proAB) Δ(mcrB-hsdSM)5(rK - mK -)\u003cbr\u003eSS320 (MC1061F'): F'[proAB lacIqZ ΔM15 Tn10 (TetR)] araD139 Δ(ara-leu)7696 galE15 galK16 Δ(lac)X74 rpsL (StrR) hsdR2 (rK– mK+) mcrA mcrB1\u003cbr\u003eER2738: [F'proA+B+ lacIq Δ(lacZ)M15 zzf::Tn10 (tetr)] fhuA2 glnVΔ(lac-proAB) thi-1Δ(hsdS-mcrB)5\u003cbr\u003eMC1061 F-: F-, araD139 Δ(ara-leu)7696 galE15 galK16 Δ(lac)X74 rpsL (StrR) hsdR2 (rK– mK+) mcrA mcrB1\u003c\/p\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eCompetent Cells include Control DNA and Recovery Medium and are packaged as DUOs (2 transformations per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.\u003c\/p\u003e\n\u003ch2\u003eResources\u003c\/h2\u003e\n\u003cp\u003eApplication notes\u003c\/p\u003e\u003cp\u003etechnical note E.coli strains for phage display\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_COMCEL-013_60512-SS320-MC1061-F-Prime-Electrocompetent-Cells\"\u003e60512 SS320 MC1061 F Prime Electrocompetent Cells\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_COMCEL-013_SDS007-60512-SS320-MC1061-F-Prime-Electrocompetent-Cells\"\u003eSDS007 60512 SS320 MC1061 F Prime Electrocompetent Cells\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_COMCEL-013_MC1061F-Electro\"\u003eMC1061F Electro\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_COMCEL-013_Phage-Display\"\u003ePhage Display\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/pis_COMCEL-013_F302-US-phage-display-comp-cells\"\u003eF302 US phage display comp cells\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/app-note_COMCEL-013_technical-note-E.coli-strains-for-phage-display\"\u003etechnical note E.coli strains for phage display\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"12 Reactions (DUOs)","offer_id":45986433597519,"sku":"60512-1","price":1390.0,"currency_code":"ILS","in_stock":true},{"title":"24 Reactions (DUOs)","offer_id":45986433630287,"sku":"60512-2","price":2288.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/SS320-_2528MC1061-F_2527_2529-Electrocompetent-Cells_ef5581a5-51bd-4dbc-9af0-612ebe277cbe.jpg?v=1785956148"},{"product_id":"mc1061-f-electrocompetent-cells-comcel-012","title":"MC1061 F-Electrocompetent Cells","description":"\u003cp\u003eMC1061 F- Electrocompetent Cells. A non-amber suppressor strain, identical to SS320 except that it lacks the F′episome. Highly efficient (≥4 × 1010 cfu\/µg) electrocompetent cells for general cloning or Phage Display. Note that as an F- strain, it cannot be used for re-infection by filamentous phage. This strain not included in the sample kit.\u003c\/p\u003e\u003cp\u003eFigure 1. Transformation efficiency of electrocompetent bacterial cells for phage display compared to competitor's specification.\u003c\/p\u003e\u003cp\u003eTable 1. Competent Cells for Phage Display libraries\u003c\/p\u003e\u003cp\u003eCell Lines\u003c\/p\u003e\u003cp\u003eTransformation Efficiency\u003cbr\u003e(cfu\/µg pUC DNA)\u003c\/p\u003e\u003cp\u003eCloning Methylated DNA\u003c\/p\u003e\u003cp\u003eBAC, Cosmid Cloning\u003c\/p\u003e\u003cp\u003eBlue\/White Screening\u003c\/p\u003e\u003cp\u003eTG1 Electrocompetent\u003c\/p\u003e\u003cp\u003e\u0026gt;4 × 1010\u003c\/p\u003e\u003cp\u003eYES\u003c\/p\u003e\u003cp\u003eNO\u003c\/p\u003e\u003cp\u003eYES\u003cbr\u003eIPTG induction required\u003c\/p\u003e\u003cp\u003eSS320 (MC1061 F') Electrocompetent\u003c\/p\u003e\u003cp\u003e\u0026gt;4× 1010\u003c\/p\u003e\u003cp\u003eYES\u003c\/p\u003e\u003cp\u003eNO\u003c\/p\u003e\u003cp\u003eYES\u003cbr\u003eIPTG induction required\u003c\/p\u003e\u003cp\u003eMC1061 F- Electrocompetent\u003c\/p\u003e\u003cp\u003e\u0026gt;4× 1010\u003c\/p\u003e\u003cp\u003eYES\u003c\/p\u003e\u003cp\u003eNO\u003c\/p\u003e\u003cp\u003eYES\u003cbr\u003eIPTG induction required\u003c\/p\u003e\u003cp\u003eER2738 Electrocompetent\u003c\/p\u003e\u003cp\u003e\u0026gt;2 × 1010\u003c\/p\u003e\u003cp\u003eYES\u003c\/p\u003e\u003cp\u003eNO\u003c\/p\u003e\u003cp\u003eYES\u003cbr\u003eIPTG induction required\u003c\/p\u003e\u003cp\u003eread more\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003ePerfect for antibody phage display or peptide phage display library creation\u003c\/li\u003e\n\u003cli\u003eThe highest efficiency TG1 competent cells available\u003c\/li\u003e\n\u003cli\u003eYour only source for electrocompetent SS320 and ER2738 cells\u003c\/li\u003e\n\u003cli\u003eAmber suppressor and non-amber suppressor lines for maximum flexibility\u003c\/li\u003e\n\u003cli\u003eHigh efficiency MC1061 F- cells for cloning or control reactions\u003c\/li\u003e\n\u003cli\u003eCreate larger libraries; speed discovery\u003c\/li\u003e\n\u003cli\u003eBulk custom dispensing available with all of our phage display cells\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cp\u003eGenotype\u003c\/p\u003e\u003cp\u003eTG1: [F' traD36 proAB lacIqZ ΔM15] supE thi-1 Δ(lac-proAB) Δ(mcrB-hsdSM)5(rK - mK -)\u003cbr\u003eSS320 (MC1061F'): F'[proAB lacIqZ ΔM15 Tn10 (TetR)] araD139 Δ(ara-leu)7696 galE15 galK16 Δ(lac)X74 rpsL (StrR) hsdR2 (rK– mK+) mcrA mcrB1\u003cbr\u003eER2738: [F'proA+B+ lacIq Δ(lacZ)M15 zzf::Tn10 (tetr)] fhuA2 glnVΔ(lac-proAB) thi-1Δ(hsdS-mcrB)5\u003cbr\u003eMC1061 F-: F-, araD139 Δ(ara-leu)7696 galE15 galK16 Δ(lac)X74 rpsL (StrR) hsdR2 (rK– mK+) mcrA mcrB1\u003c\/p\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eCompetent Cells include Control DNA and Recovery Medium and are packaged as DUOs (2 transformations per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.\u003c\/p\u003e\n\u003ch2\u003eResources\u003c\/h2\u003e\n\u003cp\u003eApplication notes\u003c\/p\u003e\u003cp\u003etechnical note E.coli strains for phage display\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_COMCEL-012_60514-1-60514-2-MC1061-F-Electrocompetent-Cells\"\u003e60514 1 60514 2 MC1061 F Electrocompetent Cells\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_COMCEL-012_60514-MC1061-F-Electrocompetent-Cells\"\u003e60514 MC1061 F Electrocompetent Cells\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_COMCEL-012_MC1061F-Electro\"\u003eMC1061F Electro\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_COMCEL-012_Phage-Display\"\u003ePhage Display\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/pis_COMCEL-012_F302-US-phage-display-comp-cells\"\u003eF302 US phage display comp cells\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/app-note_COMCEL-012_technical-note-E.coli-strains-for-phage-display\"\u003etechnical note E.coli strains for phage display\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"12 Reactions (DUOs)","offer_id":45986433663055,"sku":"60514-1","price":1219.0,"currency_code":"ILS","in_stock":true},{"title":"24 Reactions (DUOs)","offer_id":45986433695823,"sku":"60514-2","price":2097.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/MC1061-F-Electrocompetent-Cells_2820ecd1-4d77-42b7-8e8e-9ebb14367809.jpg?v=1785956152"},{"product_id":"er2738-electrocompetent-cells-comcel-005","title":"ER2738 Electrocompetent Cells","description":"\u003cp\u003eER2738 Electrocompetent Cells. An amber suppressor strain (glnV) prepared as highly efficient (≥2 × 1010 cfu\/µg) electrocompetent cells for phage display library screening. This strain is recommended for use with New England Biolab's Ph.D.™ Phage Display Kits.\u003c\/p\u003e\u003cp\u003eFigure 1. Transformation efficiency of electrocompetent bacterial cells for phage display compared to competitor's specification.\u003c\/p\u003e\u003cp\u003eTable 1. Competent Cells for Phage Display libraries\u003c\/p\u003e\u003cp\u003eCell Lines\u003c\/p\u003e\u003cp\u003eTransformation Efficiency\u003cbr\u003e(cfu\/µg pUC DNA)\u003c\/p\u003e\u003cp\u003eCloning Methylated DNA\u003c\/p\u003e\u003cp\u003eBAC, Cosmid Cloning\u003c\/p\u003e\u003cp\u003eBlue\/White Screening\u003c\/p\u003e\u003cp\u003eTG1 Electrocompetent\u003c\/p\u003e\u003cp\u003e\u0026gt;4 × 1010\u003c\/p\u003e\u003cp\u003eYES\u003c\/p\u003e\u003cp\u003eNO\u003c\/p\u003e\u003cp\u003eYES\u003cbr\u003eIPTG induction required\u003c\/p\u003e\u003cp\u003eSS320 (MC1061 F') Electrocompetent\u003c\/p\u003e\u003cp\u003e\u0026gt;4× 1010\u003c\/p\u003e\u003cp\u003eYES\u003c\/p\u003e\u003cp\u003eNO\u003c\/p\u003e\u003cp\u003eYES\u003cbr\u003eIPTG induction required\u003c\/p\u003e\u003cp\u003eMC1061 F-Electrocompetent\u003c\/p\u003e\u003cp\u003e\u0026gt;4× 1010\u003c\/p\u003e\u003cp\u003eYES\u003c\/p\u003e\u003cp\u003eNO\u003c\/p\u003e\u003cp\u003eYES\u003cbr\u003eIPTG induction required\u003c\/p\u003e\u003cp\u003eER2738 Electrocompetent\u003c\/p\u003e\u003cp\u003e\u0026gt;2 × 1010\u003c\/p\u003e\u003cp\u003eYES\u003c\/p\u003e\u003cp\u003eNO\u003c\/p\u003e\u003cp\u003eYES\u003cbr\u003eIPTG induction required\u003c\/p\u003e\u003cp\u003eread more\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003ePerfect for peptide phage display library creation\u003c\/li\u003e\n\u003cli\u003eYour only source for electrocompetent ER2738 cells\u003c\/li\u003e\n\u003cli\u003eCreate larger libraries; speed discovery\u003c\/li\u003e\n\u003cli\u003eBulk custom dispensing available with all of our phage display cells\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cp\u003eGenotype\u003c\/p\u003e\u003cp\u003eTG1: [F' traD36 proAB lacIqZ ΔM15] supE thi-1 Δ(lac-proAB) Δ(mcrB-hsdSM)5(rK - mK -)\u003cbr\u003eSS320 (MC1061F'): F'[proAB lacIqZ ΔM15 Tn10 (TetR)] araD139 Δ(ara-leu)7696 galE15 galK16 Δ(lac)X74 rpsL (StrR) hsdR2 (rK– mK+) mcrA mcrB1\u003cbr\u003eER2738: [F'proA+B+ lacIq Δ(lacZ)M15 zzf::Tn10 (tetr)] fhuA2 glnVΔ(lac-proAB) thi-1Δ(hsdS-mcrB)5\u003cbr\u003eMC1061 F-: F-, araD139 Δ(ara-leu)7696 galE15 galK16 Δ(lac)X74 rpsL (StrR) hsdR2 (rK– mK+) mcrA mcrB1\u003c\/p\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eCompetent Cells include Control DNA and Recovery Medium and are packaged as DUOs (2 transformations per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.\u003c\/p\u003e\n\u003ch2\u003eResources\u003c\/h2\u003e\n\u003cp\u003eApplication notes\u003c\/p\u003e\u003cp\u003etechnical note E.coli strains for phage display\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_COMCEL-005_60522-ER2738-Electrocompetent-Cells\"\u003e60522 ER2738 Electrocompetent Cells\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_COMCEL-005_SDS016-60522-1-60522-2-ER2738-Electrocompetent-Cells\"\u003eSDS016 60522 1 60522 2 ER2738 Electrocompetent Cells\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_COMCEL-005_MC1061F-Electro\"\u003eMC1061F Electro\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_COMCEL-005_Phage-Display\"\u003ePhage Display\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/pis_COMCEL-005_F302-US-phage-display-comp-cells\"\u003eF302 US phage display comp cells\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/app-note_COMCEL-005_technical-note-E.coli-strains-for-phage-display\"\u003etechnical note E.coli strains for phage display\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"12 Reactions (DUOs)","offer_id":45986433925199,"sku":"60522-1","price":1250.0,"currency_code":"ILS","in_stock":true},{"title":"24 Reactions (DUOs)","offer_id":45986433957967,"sku":"60522-2","price":2157.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/ER2738-Electrocompetent-Cells_27b4034d-2099-48e4-b0bd-e649ad48886c.jpg?v=1785956154"},{"product_id":"clearcoli-bl21-de3-electrocompetent-cells-comcel-008","title":"ClearColi BL21(DE3) Electrocompetent Cells","description":"\u003cp\u003eIntroduction to ClearColi® technology:\u003cbr\u003eIs there a better way to eliminate endotoxin contamination?\u003cbr\u003eNow there is.\u003c\/p\u003e\u003cp\u003eInstead of removing lipopolysaccharide (LPS) contamination from your protein or plasmid DNA preparations, eliminate the LPS at the source. Genetically modified LPS from a novel E. coli strain produces functionally clean recombinant proteins and plasmids. ClearColi® BL21(DE3) cells are the first commercially available competent cells with a modified LPS (Lipid IVA - see Fig. 1) that does not trigger the endotoxic response in mammalian cells. ClearColi™ cells lack outer membrane agonists for hTLR4\/MD-2 activation; therefore, activation of hTLR4\/MD-2 signalling by ClearColi is several orders of magnitude lower compared with E. coli wild-type cells, and heterologous proteins prepared from ClearColi are virtually free of endotoxic activity. After minimal purification from ClearColi cells, proteins or plasmids, which may still contain Lipid IVA, can still be used in most applications without eliciting an endotoxic response .\u003c\/p\u003e\u003cp\u003eFigure 1. The LPS of a normal E. coli cell compared to the genetically modified Lipid IVA from ClearColi cells. In ClearColi, the oligosaccharide chain has been deleted, and two of the six acyl chains have been removed to disable the endotoxin signal.\u003c\/p\u003e\u003cp\u003eModifications to the genotype of the ClearColi cells consist of seven separate gene deletions, thereby ensuring there is no chance of genetic reversion back to wild type and production of normal LPS. These mutations result in the deletion of the oligosaccharide chain from the LPS, making it easier to remove the resulting lipid IVA from the downstream product. More importantly, two of the six acyl chains are deleted. The six acyl chains of the LPS are the trigger which is recognised by the Toll-like receptor 4 (TLR4) in complex with myeloid differentiation factor 2 (MD-2), causing activation of NF-ƙB and production of proinflammatory cytokines. Lipid IVA, which contains only four acyl chains, is not recognised by TLR4 and thus does not trigger the endotoxic response (see Figure. 2).\u003c\/p\u003e\u003cp\u003eFig. 2. Comparison of relative NF-κB induction in HEK-Blue Cells using purified LPS from a K-12 E. coli strain or from pure, synthetically manufactured Lipid IVA.\u003c\/p\u003e\u003cp\u003eBack to top ▲\u003c\/p\u003e\u003cp\u003eGenotype Information:\u003c\/p\u003e\u003cp\u003eClearColi BL21(DE3) competent cells have the following genotype:\u003cbr\u003eF– ompT hsdSB (rB- mB-) gal dcm lon λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) msbA148 ΔgutQ ΔkdsD ΔlpxLΔlpxMΔpagPΔlpxP ΔeptA\u003c\/p\u003e\u003cp\u003eSeven specific deletion mutations (ΔgutQ ΔkdsD ΔlpxL ΔlpxMΔpagPΔlpxPΔeptA) encode the modification of LPS to Lipid IVA, while one additional compensating mutation (msbA148) enables the cells to maintain viability in the presence of the LPS precursor lipid IVA.\u003c\/p\u003e\u003cp\u003eBack to top ▲\u003c\/p\u003e\u003cp\u003eWhy Endotoxin Removal is not the Best Method\u003c\/p\u003e\u003cp\u003eLipopolysaccharide (LPS), also known as endotoxin, is a potent agonist for hTLR4\/MD-2-mediated proinflammatory activity in human immune cells. To prevent toxicity, recombinant proteins, commonly manufactured in E. coli, must be essentially free of endotoxin. However, efficient elimination of endotoxin is a challenging task, and none of the downstream applications remove endotoxin entirely. Current methods for endotoxin removal from recombinant proteins are varied, including ultra-filtration, activated carbon, surfactants, anion exchange chromatography, and immobilized sepharose. Each of these strategies involves negative effects: significant yield loss, high cost, loss of bioactivity of the protein, or bioactivity of the additives used for endotoxin clean up. Table. 1 below describes some of the most common methods and their associated shortcomings:\u003c\/p\u003e\u003cp\u003eTable 1:\u003c\/p\u003e\u003cp\u003eMethod\u003c\/p\u003e\u003cp\u003eDisadvantages\u003c\/p\u003e\u003cp\u003eUltrafiltration\u003c\/p\u003e\u003cp\u003eOnly useful for small proteins\u003cbr\u003eEndotoxin monomers may permeate the membrane due to endotoxin\/protein interaction\u003cbr\u003eInefficient for proteins which can be damaged by physical forces\u003c\/p\u003e\u003cp\u003eActivated carbon\u003c\/p\u003e\u003cp\u003eAdsorbing activity for both endotoxin and protein\u003c\/p\u003e\u003cp\u003eSurfactants\u003c\/p\u003e\u003cp\u003eExpensive\u003cbr\u003eMay affect bioactivity of protein\u003cbr\u003eDifficult to completely remove\u003cbr\u003eRemoval may lead to product loss\u003c\/p\u003e\u003cp\u003eAnion-exchange chromatography\u003c\/p\u003e\u003cp\u003eHigh adsorbtion of both endotoxin and acidic protein\u003cbr\u003eNo selectivity to adsorb endotoxin\u003c\/p\u003e\u003cp\u003eHistamine- and histidine-immobilized Sepharose\u003c\/p\u003e\u003cp\u003eRemoving capacity dependent on the ionic strength\u003cbr\u003eBiological activity of histamine\u003c\/p\u003e\u003cp\u003ePolymyxin B-immobilised Sepharose\u003c\/p\u003e\u003cp\u003eProtein losses due to the ionic interaction between polymyxin B and protein\u003cbr\u003ePolymixin B is physiologically active\u003c\/p\u003e\u003cp\u003eBack to top ▲\u003c\/p\u003e\u003cp\u003eProtein Expression with ClearColi\u003c\/p\u003e\u003cp\u003eThe relative production efficiency of endotoxin free ClearColi™ BL21(DE3) competent cells was compared to normal BL21(DE3) cells using two different recombinant proteins. Although overall growth rates of the ClearColi are slower, final protein production levels are very similar (see Figure. 3 and 4).\u003c\/p\u003e\u003cp\u003eFigure 3. Comparison of protein expression in ClearColi BL21(DE3) and Lucigen's E. Cloni® EXPRESS BL21(DE3) competent cells. Cells containing a T7 expression plasmid harbouring a gene encoding the human apolipoprotein A1 (ApoA1) were grown in LB Miller medium at 37 °C. When cultures reached OD600 of 0.6 to 0.8, expression was induced by the addition of 0.4 mM IPTG and incubation was continued for 3 hours. Equivalent numbers of uninduced (-) and induced (+) cells were lysed by heating in Laemmli buffer and samples were analysed by SDS-PAGE on a 4% - 20% polyacrylamide gradient gel.\u003c\/p\u003e\u003cp\u003eFigure 4. Comparison of fluorescent protein expression in ClearColi BL21(DE3) and E. cloni EXPRESS BL21(DE3) cells. A pET expression vector harbouring a gene encoding a yellow fluorescent protein (LucY) under the control of a T7 promoter was transformed into both ClearColi BL21(DE3) and E. cloni EXPRESS BL21(DE3). Colonies were inoculated into LB-Miller medium and grown at 37 °C for induction. Samples of induced and uninduced cells containing equivalent numbers of cells were centrifuged, and cell pellets were photographed under long-wavelength UV illumination.\u003c\/p\u003e\u003cp\u003eBack to top ▲\u003c\/p\u003e\u003cp\u003eEndotoxicity Assay Results\u003c\/p\u003e\u003cp\u003eIn most cases, a simple nickel column purification is sufficient for endotoxic shock response negative recombinant proteins suitable or downstream eukaryotic cellular response assays. Researchers can now assay target protein effects without concern that immune response triggers are a result of LPS endotoxin contamination. To demonstrate, ApoA1 protein expressed from ClearColi BL21(DE3) competent cells was Ni-column purified and tested for endotoxicity using both the HEK-Blue cell line from Invivogen (see Fig 5) and the LAL assay (see Fig. 6).\u003c\/p\u003e\u003cp\u003eFigure 5. Comparison of endotoxic response from protein derived from ClearColi BL21(DE3) and traditional BL21(DE3) competent cells\u003c\/p\u003e\u003cp\u003eBack to top ▲\u003c\/p\u003e\u003cp\u003eLAL Testing: Does it really measure endotoxin?\u003c\/p\u003e\u003cp\u003eLimulus amebocyte assay testing is an FDA-approved method for detection of endotoxins and the most common assay used; however the LAL assay is activated solely by the 4´-monophosphoryldiglucosamine backbone of LPS. LAL activity is minimally influenced by acylation pattern of LPS, the key determinant of endotoxicity in eukaryotic cells. The LAL assay also recognizes a wider spectrum of LPS\/lipid A variants than the central cellular endotoxin sensor system of the human immune cell system. As such, false positive results can and will result due to the lack of specificity of the assay.\u003c\/p\u003e\u003cp\u003eA simple Ni-column purification step for proteins produced from ClearColi cells will reduce LAL response levels by 95% or greater (see Fig. 6). However, the residual EU measurements are due to the non-specific nature of the assay unless extraneous LPS contamination from other sources is present. Alternative toxicity assays, such as those using HEK-Blue cells (see Fig. 5) suggest that even in the presence of EU levels above threshholds normally targeted by researchers, the actual immunogenic effects from ClearColi-derived proteins are non-existent.\u003c\/p\u003e\u003cp\u003eDue to the non-specificity of the LAL assay when combined with lipid IVA from ClearColi, it is suggested that researchers consider alternative methods of endotoxin measurement.\u003c\/p\u003e\u003cp\u003eFigure 6. Comparison of endotoxin units as measured by the LAL assay using nickel-column purified recombinant ApoA1 and HSP protein expressed in ClearColi BL21(DE3) (red bars) and E. cloni EXPRESS BL21(DE3) (grey bars) competent cells. Protein expressed from ClearColi demonstrates significant reduction in EU\/mg without endotoxin removal steps.\u003c\/p\u003e\u003cp\u003eBack to top ▲\u003c\/p\u003e\u003cp\u003eGrowth Rates for ClearColi BL21(DE3) Cells\u003c\/p\u003e\u003cp\u003eClearColi BL21(DE3) cells grow at approximately 50% of the rate of normal BL21(DE3) cells (see Fig. 7). Users should expect to see very small colonies for the first 24 hours after plating transformants. We recommend incubating plates for 32-40 hours before picking colonies for future experiments. When grown to sufficient densities, ClearColi BL21(DE3) cells produce similar protein levels as normal BL21(DE3) cells when comparing equal numbers of cells.\u003c\/p\u003e\u003cp\u003eFigure 7. Comparison of growth rates for ClearColi BL21(DE3) vs. E. cloni EXPRESS BL21(DE3) cells. Cells were inoculated to an initial OD600 of ~0.003 in 200 ml of LB Miller medium and grown at 37° C with shaking at 210 rpm. The OD600 of the cultures was recorded hourly.\u003c\/p\u003e\u003cp\u003eBack to top ▲\u003c\/p\u003e\u003cp\u003eRelevant Reference Articles:\u003cbr\u003eTeghanemt, et al, Molecular Basis of Reduced Potency of Underacylated Endotoxins, J Immunol, 2005; 175:4669-4676\u003cbr\u003eMamat, et al, Single amino acid substitutions in either YhjD or MsbA confer viability to 3-deoxy-D-manno-oct-2-ulosonic acid-depleted Escherichia coli, Molecular Microbiology, 2008, 67(3), 633–648\u003cbr\u003eMeredith, et al, Redefining the Requisite Lipopolysaccharide Structure in Escherichia coli, ACS Chemical Biology, 2006, 1(1), 33-42\u003cbr\u003eBrandenburg, et al, The Expression of Endotoxic Activity in the Limulus Test as Compared to Cytokine Production in Immune Cells, Current Medicinal Chemistry, 2009, 16, 2653-2660\u003cbr\u003eGutsmann, et al. Structural prerequisites for endotoxic activity in the Limulus test as compared to cytokine production in mononuclear cells, Innate Immunity, 2010, 16(1), 39-47\u003cbr\u003eBeom Seok Park1 et al., The structural basis of lipopolysaccharide recognition by the TLR4–MD-2 complex, Nature 458, 1191-1195 (30 April 2009)\u003c\/p\u003e\u003cp\u003eBack to top ▲\u003c\/p\u003e\u003cp\u003eClearColi Licensing Information:\u003c\/p\u003e\u003cp\u003eClearColi™ Competent cells are subject to US Patent 8,303,964 and other US and foreign pending patents.\u003c\/p\u003e\u003cp\u003eLucigen Corporation (\"Lucigen\") has a license from Research Corporation Technologies to sell ClearColi competent cells to third-parties for non-commercial research purposes only. A separate license is required for any commercial use. For more information about the use of this product by commercial entities, please review our full licensing page.\u003c\/p\u003e\u003cp\u003eBack to top ▲\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eEliminate endotoxin at the source\u003c\/li\u003e\n\u003cli\u003eGenetically modified LPS does not trigger endotoxic response in human cells\u003c\/li\u003e\n\u003cli\u003eIdeal for mammalian cell immunogenicity testing, toxicity assays, and therapeutic protein drug discovery\u003c\/li\u003e\n\u003cli\u003eUseful for membrane and lipid binding protein production\u003c\/li\u003e\n\u003cli\u003eProtein expression similar to BL21(DE3) cells without requiring downstream endotoxin removal\u003c\/li\u003e\n\u003cli\u003eReduce false positives in cytokine assays, improve confidence in your results\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cp\u003eTransformation efficiency: \u003cbr\u003e1 × 109 cfu\/ug, measured using pUC19 DNA in standard electroporation conditions\u003c\/p\u003e\u003cp\u003eGenotype:\u003cbr\u003eF– ompT hsdSB (rB- mB-) gal dcm lon λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) msbA148 ΔgutQΔkdsD ΔlpxLΔlpxMΔpagPΔlpxPΔeptA\u003c\/p\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eEach ClearColi® BL21(DE3) Electrocompetent Cell Kit contains: ClearColi BL21(DE3) Electrocompetent Cells in DUO packaging (2 transformations per tube), Expression Recovery Medium (lactose minus), pUC19 Positive Control Plasmid, and complete protocols.\u003c\/p\u003e\u003cp\u003eExpression Recovery Medium (lactose minus) is also available separately.\u003c\/p\u003e\n\u003ch2\u003eResources\u003c\/h2\u003e\n\u003cp\u003ePosters\u003c\/p\u003e\u003cp\u003eClearColi Poster Aug 2013\u003c\/p\u003e\n\u003ch2\u003eFAQs\u003c\/h2\u003e\n\u003cp\u003eFAQs\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_COMCEL-008_60810-ClearColi-BL21-DE3-Electrocompetent-Cells\"\u003e60810 ClearColi BL21 DE3 Electrocompetent Cells\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_COMCEL-008_SDS009-60850-ClearColi-K-12-Electrocompetent-Cells\"\u003eSDS009 60850 ClearColi K 12 Electrocompetent Cells\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_COMCEL-008_ClearColi-BL21-DE3-Electocompetent-Cells\"\u003eClearColi BL21 DE3 Electocompetent Cells\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/pis_COMCEL-008_F303-US-ClearColi\"\u003eF303 US ClearColi\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/poster_COMCEL-008_ClearColi-Poster-Aug-2013\"\u003eClearColi Poster Aug 2013\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"12 Reactions (DUOs)","offer_id":45986434089039,"sku":"60810-1","price":2037.0,"currency_code":"ILS","in_stock":true},{"title":"24 Reactions (DUOs)","offer_id":45986434121807,"sku":"60810-2","price":3722.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/ClearColi-BL21_2528DE3_2529-Electrocompetent-Cells_fbc504b7-5b0a-45b3-9110-f14cdc5021bb.jpg?v=1785956158"},{"product_id":"e-cloni-5-alpha-chemically-competent-cells-comcel-006","title":"E. cloni 5-alpha Chemically Competent Cells","description":"\u003cp\u003eE. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5α™ and directly replace them in cloning protocols. E. cloni chemically competent cells provide the performance researchers need with ease of use—only common laboratory equipment is required. These cells provide solutions for a wide range of applications at economical prices.\u003c\/p\u003e\u003cp\u003eE. cloni 5-alpha Chemically Competent Cells \u0026gt;1 × 10 8 cfu\/µg DNA\u003cbr\u003eUseful for routine cloning, subcloning, and plasmid isolation with or without blue\/white screening.\u003c\/p\u003e\u003cp\u003eTable 1. E. cloni Competent Cells for Cloning\u003c\/p\u003e\u003cp\u003eE. cloni Cell Lines\u003c\/p\u003e\u003cp\u003eTransformation Efficiency\u003cbr\u003e(cfu\/µg pUC DNA)\u003c\/p\u003e\u003cp\u003eCloning Methylated DNA\u003c\/p\u003e\u003cp\u003eBAC, Cosmid Cloning\u003c\/p\u003e\u003cp\u003eBlue\/White Screening\u003c\/p\u003e\u003cp\u003e5-alpha Chemically Competent\u003c\/p\u003e\u003cp\u003e\u0026gt;1 × 10 8 cfu\/µg DNA\u003c\/p\u003e\u003cp\u003eNO\u003c\/p\u003e\u003cp\u003eNO\u003c\/p\u003e\u003cp\u003eYES\u003cbr\u003eNo IPTG induction required\u003c\/p\u003e\u003cp\u003eread more\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eDirect replacements for standard cloning strains\u003c\/li\u003e\n\u003cli\u003eOptimised genetics for high yields: endonuclease and recombination minus, for blue\/white screening-capable.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cp\u003eGenotype\u003c\/p\u003e\u003cp\u003eE. cloni 5-alpha: fhuA2Δ(argF-lacZ)U169 phoA glnV44 Φ80 Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17\u003c\/p\u003e\u003cp\u003eEfficiencies\u003c\/p\u003e\u003cp\u003eE. cloni 5-alpha Chemically Competent Cells \u0026gt;1 × 108 cfu\/µg DNA\u003c\/p\u003e\u003cp\u003eread more\u003c\/p\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eCompetent Cells include Control DNA and Recovery Medium, and are packaged as DUOs (2 transformations per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_COMCEL-006_E-cloni-alpha-Chemically-Competent-Cells\"\u003eE cloni alpha Chemically Competent Cells\u003c\/a\u003e\u003c\/li\u003e\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"12 Reactions (DUOs)","offer_id":45986434154575,"sku":"60602-1","price":744.0,"currency_code":"ILS","in_stock":true},{"title":"24 Reactions (DUOs)","offer_id":45986434187343,"sku":"60602-2","price":1273.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/E-cloni-5-alpha-Chemically-Competent-Cells_6246d4d4-8d33-4fe2-8889-23959ca92e0c.jpg?v=1785956158"},{"product_id":"expression-recovery-medium-80030-1","title":"Expression Recovery Medium","description":"\u003cp\u003eOptimised formulation for ensuring stability and high-efficiency transformation of protein expression clones.\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRecommended for transformation of T7 expression clones\u003c\/li\u003e\n\u003cli\u003eFormulated without lactose to prevent unwanted leaky expression from T7 or other lactose\/IPTG-inducible promoters.\u003c\/li\u003e\n\u003cli\u003eEnables the highest possible transformation efficiency.\u003c\/li\u003e\n\u003cli\u003eUse in the outgrowth stage of competent cell transformation to achieve 10-folder higher transformation efficiency than you would get with LB or SOC media.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cp\u003eExpression Recovery Medium is tested to ensure high-efficiency competent cell transformation and to ensure a lack of contamination.\u003c\/p\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eExpression Recovery Medium is provided as one box containing eight 12 mL vials of recovery medium. Expression Recovery Medium is shipped frozen and should be stored at -20 °C until just before use.\u003c\/p\u003e\u003cp\u003eFor research use only. Not for human or diagnostic use.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_80030-1_SDS-80030-Expression-Recovery-Medium\"\u003eSDS 80030 Expression Recovery Medium\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_80030-1_SDS005--80030-0-80030-1-Expression-Recovery-Medium\"\u003eSDS005 80030 0 80030 1 Expression Recovery Medium\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"Default Title","offer_id":45986434285647,"sku":"80030-1","price":787.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/Expression-Recovery-Medium_76ff405a-0426-42ff-9c04-b8f86ceaa583.jpg?v=1785956166"},{"product_id":"recovery-medium-8-x-12-ml-rm02","title":"Recovery Medium, 8 x 12 mL","description":"\u003cp\u003eRecovery Medium is our optimized formulation, designed to produce highest possible transformation efficiencies for cloning applications.\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eEnables the highest possible transformation efficiency.\u003c\/li\u003e\n\u003cli\u003eUse in the outgrowth stage of competent cell transformation to attain up to 10-folder higher transformation efficiency than with LB or SOC media.\u003c\/li\u003e\n\u003cli\u003eTrusted formulation used for cloning with Biosearch Technologies competent cells.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_80026-1_SDS-80026-Recovery-Medium\"\u003eSDS 80026 Recovery Medium\u003c\/a\u003e\u003c\/li\u003e\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"Default Title","offer_id":45986434318415,"sku":"80026-1","price":787.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/Recovery-Medium_b5775d0d-9563-4be3-b33c-8ae21ccfc98c.jpg?v=1785956163"},{"product_id":"ampligase-thermostable-dna-ligase-a0110","title":"Ampligase Thermostable DNA Ligase","description":"\u003cp\u003eAmpligase™ Thermostable DNA Ligase catalyses NAD-dependent ligation of adjacent 3´-hydroxylated and 5´-phosphorylated termini in duplex DNA structures that are stable at high temperatures. Derived from a thermophilic bacterium, the enzyme is stable and active at much higher temperatures than conventional DNA ligases. Its half-life is 48 hours at 65°C and greater than 1 hour at 95°C. Ampligase DNA Ligase has been shown to be active for at least 500 thermal cycles (94°C\/80°C) or 16 hours of cycling. 10 This exceptional thermostability permits extremely high hybridization stringency and ligation specificity. Ampligase DNA Ligase has no detectable activity in ligating blunt-ended DNA and has no activity on RNA or RNA:DNA hybrids.\u003c\/p\u003e\u003cp\u003eApplications\u003c\/p\u003e\u003cp\u003eLigation amplification (Ligase Chain Reaction, LCR) 1-7\u003cbr\u003eRepeat Expansion Detection (RED) 7\u003cbr\u003eSimultaneous mutagenesis of multiple sites 8,9\u003cbr\u003eOther ligation-based detection methods\u003c\/p\u003e\u003cp\u003eReferences\u003c\/p\u003e\u003cp\u003eLandegren, U. et al. (1988) Science 242, 229.\u003cbr\u003eWu, D.Y. and Wallace, R.B. (1989) Genomics 4, 560.\u003cbr\u003eBarany, F. (1991) Proc. Natl. Acad. Sci. USA 88, 189.\u003cbr\u003eBirkenmeyer, L. and Armstrong, A.S. (1992) J. Clin. Micro. 30, 3089.\u003cbr\u003eDille, B.J. et al. (1993) J. Clin. Micro. 31, 729\u003cbr\u003eNakazawa, H. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 360.\u003cbr\u003eSirugo, G. and Kidd, K. (1995) Epicentre Forum 2(3), 1.\u003cbr\u003eMoore, D. and Michael, S. (1995) Epicentre Forum 2(4), 4.\u003cbr\u003eRouwendal, G.J. et al. (1993) BioTechniques 15, 68.\u003cbr\u003eSchalling, M. et al. (1993) Nature Genetics 4, 135.\u003cbr\u003eHardenbol, P. et al. (2003) Nature Biotechnology 21, 673.\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eHigh thermostability allows ligation using high-stringency hybridization conditions\u003c\/li\u003e\n\u003cli\u003eHigh specificity and stringency permits sensitive detection of SNPs\u003c\/li\u003e\n\u003cli\u003eAvailable in kit and \"enzyme only\" formats11\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eAmpligase Thermostable DNA Ligase starter Kit (Item ID A8101) includes Ampligase Thermostable DNA Ligase, 10X Ampligase Reaction Buffer, and a DNA ligation control. All other Ampligase Thermostable DNA Ligase Kits include Ampligase DNA Ligase and 10X Ampligase Reaction Buffer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_A0110_A0102K-A32750-A3202K-Ampligase-Enzyme-and-Buffer\"\u003eA0102K A32750 A3202K Ampligase Enzyme and Buffer\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/SDS_A0110_A0110K-A3210K-Ampligase-DNA-Ligase\"\u003eA0110K A3210K Ampligase DNA Ligase\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_A1905B_MA023E-Ampligase-Thermostable-DNA-Ligase\"\u003eMA023E Ampligase Thermostable DNA Ligase\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"100 U\/µL, 2,500 units","offer_id":45986434416719,"sku":"A0102K","price":1977.0,"currency_code":"ILS","in_stock":true},{"title":"100 U\/uL, 10,000 U","offer_id":45986434449487,"sku":"A0110K","price":5085.0,"currency_code":"ILS","in_stock":true},{"title":"5 U\/µL, 100,000 U","offer_id":45986434482255,"sku":"A3100","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"2,500 U @ 5 U\/µL","offer_id":45986434515023,"sku":"A3202K","price":2080.0,"currency_code":"ILS","in_stock":true},{"title":"5 U\/µL, 10,000 U","offer_id":45986434547791,"sku":"A3210K","price":5176.0,"currency_code":"ILS","in_stock":true},{"title":"5 U\/µL, 750 U","offer_id":45986434580559,"sku":"A32750","price":965.0,"currency_code":"ILS","in_stock":true},{"title":"5 U\/µL, 1,000 U","offer_id":45986434613327,"sku":"A8101","price":1350.0,"currency_code":"ILS","in_stock":true},{"title":"5 U\/uL, 20 mL, 100,000 U","offer_id":45986434646095,"sku":"E0001-5D5","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/Ampligase-Thermostable-DNA-Ligase_d0f154b6-ab31-4a17-b536-ca6f3ef116c9.jpg?v=1785956168"},{"product_id":"ampligase-10x-reaction-buffer-a1905","title":"Ampligase 10X Reaction buffer","description":"\u003cp\u003eThe Ampligase™ 10X reaction buffer is optimised for use with the Ampligase Thermostable DNA Ligase enzyme and will enable high temperature, high-specificity ligation of DNA templates when used in conjunction with the enzyme.\u003c\/p\u003e\u003cp\u003ePlease visit the Ampligase Thermostable DNA Ligase webpage for additional information on this powerful enzyme.\u003c\/p\u003e\u003cp\u003e10X Ampligase Reaction Buffer: 200 mM Tris-HCl (pH 8.3), 250 mM KCl, 100 mM MgCl2, 5 mM NAD, and 0.1% Triton® X-100.\u003c\/p\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003ePlease note this product does not include any Ampligase Thermostable DNA Ligase enzyme.\u003c\/p\u003e\u003cp\u003eAmpligase DNA Ligase Kit contains: Ampligase DNA Ligase, 10X Reaction Buffer, and Control DNA. Ampligase Enzyme \u0026amp; Buffer contain: Ampligase DNA Ligase and 10X Reaction Buffer (25 μL Buffer for each 50 U enzyme).\u003c\/p\u003e\u003cp\u003eOne unit of Ampligase is equal to as many as 15 units of other thermostable DNA ligases. Please compare competitive unit definitions.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_A1905B_SDS-A0102K-A32750-A3202K-Ampligase-Enzyme-and-Buffer\"\u003eSDS A0102K A32750 A3202K Ampligase Enzyme and Buffer\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_A1905B_SDS-A1905B-Ampligase-10X-Reaction-Buffer\"\u003eSDS A1905B Ampligase 10X Reaction Buffer\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_A1905B_MA023E-Ampligase-Thermostable-DNA-Ligase\"\u003eMA023E Ampligase Thermostable DNA Ligase\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"Default Title","offer_id":45986434678863,"sku":"A1905B","price":516.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/Ampligase-10X-Reaction-Buffer_922ab96c-47fc-4518-bf59-f868da67c61a.jpg?v=1785956170"},{"product_id":"ampliscribe-t7-high-yield-transcription-kit-as3107","title":"AmpliScribe T7 High Yield Transcription Kit","description":"\u003cp\u003eThe AmpliScribe™ T7 High Yield Transcription Kit is specially formulated to use high concentrations of NTPs, that can be inhibitory to other in vitro transcription systems. AmpliScribe High Yield Transcription reactions can produce \u0026gt;20-fold more full-length RNA transcript than conventional in vitro transcription reactions. For example, the AmpliScribe T7 Kit yields approximately 5-7.5 mg\/ml of 1.4-kb full-length control RNA in 30-120 minutes, respectively, using 1 µg of template DNA (Figure. 1).\u003c\/p\u003e\u003cp\u003eApplications\u003c\/p\u003e\u003cp\u003eHigh-yield synthesis of RNA from DNA cloned downstream from a T7 RNA polymerase promoter, for microinjection, RNA interference, or antisense experiments.\u003cbr\u003eStructural and functional studies of splicing, processing, heterogeneous nuclear RNAs, tRNAs, viral RNAs, and ribozymes. 1\u003cbr\u003eProduction of fluorescent 2-, biotinylated 3-, or digoxigenin 4- labelled RNA.\u003cbr\u003eFeatures\u003c\/p\u003e\u003cp\u003eHigh yield. Produce microgram amounts of RNA from all templates—synthesise both long (Figure. 2) and short RNA using only one kit. 5\u003cbr\u003eSensitive. Produce microgram amounts of RNA from as little as 1 ng of template.\u003cbr\u003eScalable. Increase the reaction size to produce milligram amounts of RNA.\u003cbr\u003eFlexible. Substitute a labelled nucleotide for one of the supplied NTP solutions.\u003cbr\u003eConvenient. RNase inhibitor included.\u003c\/p\u003e\u003cp\u003eNote: Because of the very high concentration of NTPs used in AmpliScribe reactions, these kits are not recommended for preparing high specific-activity radiolabelled RNA probes.\u003c\/p\u003e\u003cp\u003eFigure 1. The AmpliScribe™ T7 High Yield Transcription Kit is specially formulated to consistently produce \u0026gt;20-fold more RNA than a conventional T7 in vitro transcription reaction.\u003cbr\u003eFigure 2. An AmpliScribe™ T7 High Yield Transcription reaction produces full-length, long RNA transcripts. Aliquots from standard 20 µl AmpliScribe T7 High Yield Transcription reactions were loaded onto a denaturing 1% agarose-formaldehyde gel 6 and stained with ethidium bromide. The size of the RNA transcripts and RNA yields (in parentheses) were: Lane 1, 3.0 kb (185 µg); lane 2, 4.0 kb (178 µg); lane 3, 5.0 kb (176 µg); lane 4, 6.0 kb (169 µg); lane M, RNA size marker.\u003c\/p\u003e\u003cp\u003eReferences\u003c\/p\u003e\u003cp\u003eJohnson, M. (1995) Epicentre Forum 2(5), 4.\u003cbr\u003eDeLong, E.F. et al. (1999) App. And Environ. Microbiol. 65, 5554.\u003cbr\u003eKaplan, E. et al. (1996) Epicentre Forum 3(2), 1.\u003cbr\u003eHoff man, L.M. and Johnson, M.G. (1994) BioTechniques 17, 372.\u003cbr\u003eSchanke, J. (2000) Epicentre Forum 7(2), 6.\u003cbr\u003eMolecular Cloning - A Laboratory Manual, Third Edition, 2001. CSHL Press. pp 7.27 - 7.34. J. Sambrook and D. Russell.\u003cbr\u003eread more\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eProduce high yields of high quality RNA quickly and easily.\u003c\/li\u003e\n\u003cli\u003eSynthesise long and short RNA with one kit\u003c\/li\u003e\n\u003cli\u003eProduce 20 times more RNA than conventional or \"homemade\" in vitro transcription kits\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eAmpliScribe T7 Enzyme Solution, 100 mM ATP, CTP, GTP, and UTP Solutions, AmpliScribe 10X Reaction Buffer, 100 mM Dithiothreitol (DTT), RiboGuard RNase Inhibitor, RNase-Free Water, Control Template DNA, and RNase-Free DNase I\u003c\/p\u003e\n\u003ch2\u003eFAQs\u003c\/h2\u003e\n\u003cp\u003eFAQs\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_AS3107_SDS-AS3107-AmpliScribe-T7-Flash-High-Yield-Transcription-Kit\"\u003eSDS AS3107 AmpliScribe T7 Flash High Yield Transcription Kit\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_AS3107_MA040E-AmpliScribe-Kits\"\u003eMA040E AmpliScribe Kits\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"Default Title","offer_id":45986435301455,"sku":"AS3107","price":2791.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/AmpliScribe-T7-High-Yield-Transcription-Kit_82ef14f1-d961-463f-a78f-124819bf7523.jpg?v=1785956172"},{"product_id":"ampliscribe-t7-flash-transcription-kits-mbtool-006","title":"AmpliScribe T7-Flash Transcription Kits","description":"\u003cp\u003eAn AmpliScribe T7-Flash Transcription reaction yields more RNA (180 µg per 20-µl reaction) than other high-yield in vitro transcription kit reactions. The AmpliScribe T7-Flash Kit utilizes proprietary enzyme formulations that enable the maximum possible yields of RNA from virtually any template that is downstream of the T7 RNA polymerase promoter, including sequences in linearized plasmids, double-stranded oligos or PCR products.\u003c\/p\u003e\u003cp\u003eFeatures\u003c\/p\u003e\u003cp\u003eExceptionally high yields. Up to 180 µg of full-length RNA can be produced from 1 µg of 1-kb linearized plasmid DNA template.\u003cbr\u003eScalable. Reactions can be scaled up to obtain grams (or more) of RNA.\u003cbr\u003eFast. A standard AmpliScribe T7-Flash reaction is complete in 30 minutes.\u003cbr\u003eConvenient. Rnase inhibitor included.\u003cbr\u003eTrusted. Can be used for classic \"Eberwine\"-type RNA amplification procedures.\u003cbr\u003eread more\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eGenerate maximum amounts of RNA - over 180 ug RNA from 1 ug DNA template\u003c\/li\u003e\n\u003cli\u003eSave time with 30 minute high-yield protocol\u003c\/li\u003e\n\u003cli\u003eScale up the reaction to produce grams of RNA\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eAmpliScribe T7-Flash Transcription Kits ASF3257(25 reactions) and ASF3507 (50 reactions) contain AmpliScribe T7-Flash Enzyme Solution, AmpliScribe T7-Flash 10X Reaction Buffer, ATP, CTP, GTP and UTP Solutions, RNase-Free Water, RNase-Free DNase I, DTT, Control DNA Template (linearized), and RiboGuard RNase Inhibitor. AmpliScribe T7-Flash Transcription Kit Item ID ASF250 (250 reactions) contains AmpliScribe T7-Flash Enzyme Solution and AmpliScribe T7-Flash 10X Reaction Buffer only.-\u003c\/p\u003e\u003cp\u003eThe \"enzyme and 10X buffer only\" format includes AmpliScribe T7-Flash Enzyme Solution andd 10X Reaction Buffer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_MBTOOL-006_ASF3257-AmpliScribe-T7-Flash-Transcription-Kit\"\u003eASF3257 AmpliScribe T7 Flash Transcription Kit\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_MBTOOL-006_ASF3507-AmpliScribe-T7-Flash-Transcription-Kit\"\u003eASF3507 AmpliScribe T7 Flash Transcription Kit\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_MBTOOL-006_Ampliscribe-t7-flash-transcription-kit\"\u003eAmpliscribe t7 flash transcription kit\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/pis_MBTOOL-006_F500-US-AmpliScribe-T7-Flash\"\u003eF500 US AmpliScribe T7 Flash\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"250 Reactions","offer_id":45986439135311,"sku":"ASF250","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"25 Rxns","offer_id":45986439168079,"sku":"ASF3257","price":2087.0,"currency_code":"ILS","in_stock":true},{"title":"50 Rxns","offer_id":45986439200847,"sku":"ASF3507","price":3035.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/AmpliScribe-T7-Flash-Transcription-Kits_246e4841-ae21-4e1d-b4f0-704021db57b2.jpg?v=1785956175"},{"product_id":"copycutter-epi400-competent-cells-comcel-010","title":"CopyCutter EPI400 Competent Cells","description":"\u003cp\u003eCopyCutter™ EPI400™ E. coli* cells were developed to significantly lower the copy number of a wide variety of common vectors so that you can more readily clone unstable DNA sequences. DNA that is unstable at high-copy number often codes for a protein that inhibits cell growth or contains AT- and GC-rich sequences or sequences with strong secondary structure (Figure. 2).1\u003c\/p\u003e\u003cp\u003eApplications\u003c\/p\u003e\u003cp\u003eCloning of unstable DNA sequences or those expressing toxic proteins.\u003c\/p\u003e\u003cp\u003eThe CopyCutter EPI400 cell line was derived from Epicentre's high-transformation efficiency phage T1-resistant TransforMAX™ EC100™-T1RE.coli strain by manipulating a gene that controls the copy number of vectors containing ColE1 or pMB1 origins of replication (e.g., pUC- and pET-type vectors). This constitutively expressed gene, pcnB (plasmid copy number), was deleted from the TransforMAX EC100 strain and replaced with a modified pcnB gene that is linked to an inducible promoter, creating the CopyCutter EPI400 strain.\u003c\/p\u003e\u003cp\u003eThe copy number of ColE1-type vectors in the CopyCutter EPI400 strain compared to the parental TransforMAX EC100 strain is approximately 4- to 25-fold lower, depending on the vector. Moreover, a short incubation in the presence of the CopyCutter Induction Solution can increase the copy number of the vector to improve plasmid yields (Figure. 3).\u003c\/p\u003e\u003cp\u003eFigure 1. Copy-number of ColE1-type plasmids is lowered up to 25-fold in CopyCutter EPI400 E. coli cells. Lanes C, TransforMax™ EC100™ cells; Lanes U and I, uninduced or induced CopyCutter EPI400 cells. DNA extracts from the same number of lysed cells (based on OD600) were loaded per lane.\u003c\/p\u003e\u003cp\u003eFigure 2. DNA inserts encoding toxic gene products were successfully cloned into high-copy-number vectors using CopyCutter EPI400 E. coli cells. After sequencing, the full-length acpP clones in TransforMAX EC100 cells were found to contain multiple point mutations.\tFigure 3. Uninduced CopyCutter EPI400 E. coli cells containing a regB clone (lane U) are induced to higher-copy number (lane I) using the CopyCutter Induction Solution. Crude extracts of plasmid DNA were prepared from cells grown in selective media and analysed by agarose gel electrophoresis. Approximately the same number of lysed cells (based on A600) were loaded per lane.\u003c\/p\u003e\u003cp\u003eBenefits\u003c\/p\u003e\u003cp\u003eMaintain clones at low-copy number, then induce to higher copy number for improved plasmid yield.\u003cbr\u003eHigh transformation efficiency with clones of all sizes.\u003cbr\u003etonA for resistance to bacteriophages T1 and T5.\u003cbr\u003elacZΔM15 for blue\/white screening of recombinants.\u003cbr\u003eRestriction minus [mcrA, Δ(mrr-hsdRMS-mcrBC)] genotype enables efficient cloning of methylated DNA.\u003cbr\u003eEndonuclease minus (endA1) to ensure high yields of DNA.\u003cbr\u003eRecombination minus (recA1) for greater stability of large cloned inserts.\u003c\/p\u003e\u003cp\u003eGenotype\u003c\/p\u003e\u003cp\u003eF- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL (StrR) nupG trfA tonA pcnB4 dhfr\u003c\/p\u003e\u003cp\u003eCopyCutter EPI400 Electrocompetent E. coli\u003c\/p\u003e\u003cp\u003eTransformation efficiency of \u0026gt;1 x 1010 cfu\/µg of pUC19.\u003c\/p\u003e\u003cp\u003eCopyCutter EPI400 Chemically Competent E. coli\u003c\/p\u003e\u003cp\u003eTransformation efficiency of \u0026gt;1 x 107 cfu\/µg of pUC19.\u003c\/p\u003e\u003cp\u003eReference\u003c\/p\u003e\u003cp\u003eHaskins, D. (2004) Epicentre Forum 11(5), 6.\u003c\/p\u003e\u003cp\u003e*Covered by issued and\/or pending patents.\u003c\/p\u003e\u003cp\u003eread more\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eOptimised for cloning toxic or unstable DNA\u003c\/li\u003e\n\u003cli\u003eStabilise toxic inserts in common cloning and expression vectors (pUC and pET-type vectors)\u003c\/li\u003e\n\u003cli\u003eClone and maintain challenging sequences at reduced plasmid copy number, then induce to high copy number for DNA recovery\u003c\/li\u003e\n\u003cli\u003eAvoid T1 and T5 phage contamination with tonA mutation\u003c\/li\u003e\n\u003cli\u003eChoose chemically competent cells for general cloning or electrocompetent cells for demanding applications such as library generation\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eTen tubes each containing 50 µL of cells (enough cells for 10 transformations), CopyCutter Induction Solution and pUC19 control DNA. The CopyCutterInduction Solution is provided at a 1,000X concentration and is filter sterilised.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_COMCEL-010_C400CH10-CopyCutter-EPI400-Chemically-Competent-E-coli\"\u003eC400CH10 CopyCutter EPI400 Chemically Competent E coli\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_COMCEL-010_C400EL10-CopyCutter-EPI400-Electrocompetent-Ecoli\"\u003eC400EL10 CopyCutter EPI400 Electrocompetent Ecoli\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_COMCEL-010_CopyCutter-cells-\u0026amp;-Induction-Soln\"\u003eCopyCutter cells \u0026amp; Induction Soln\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/pis_COMCEL-010_F306-US-CopyCutter-EPIT400-Comp-Cells-X-1a\"\u003eF306 US CopyCutter EPIT400 Comp Cells X 1a\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"10 Reactions","offer_id":45986439397455,"sku":"C400CH10","price":1250.0,"currency_code":"ILS","in_stock":true},{"title":"10 Reactions (C400EL10)","offer_id":45986439430223,"sku":"C400EL10","price":1658.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/CopyCutter-EPI400-Competent-Cells_df34fd19-d77c-45f5-8221-749046b92c12.jpg?v=1785956183"},{"product_id":"transformax-epi300-comcel-009","title":"TransforMax EPI300","description":"\u003cp\u003eTransforMax™ EPI300™ E. coli cells lack the tonA gene and are engineered for use with Epicentre's CopyControl™ cDNA, Gene, and PCR Cloning Kit and other CopyControl Cloning Systems* that do not require phage T1-resistant cells. The cells contain an inducible mutant trfA gene whose gene product is required for initiation of replication from the oriV origin of replication, such as that in CopyControl pCC1™ vectors. On LB chloramphenicol plates or in LB or SOC media supplemented with chloramphenicol, CopyControl clones grown in TransforMax EPI300 E. coli replicate at single-copy number from the F-factor replicon because expression of the trfA gene is repressed. Addition of CopyControl Induction Solution induces the cells to express the trfA gene product and induces their replication at high-copy number from oriV (Figure. 1). Replication from oriV results in higher yields and higher purity of cloned DNA.\u003c\/p\u003e\u003cp\u003eBenefits\u003c\/p\u003e\u003cp\u003eCopy-number of clones is under tight control of an inducible promoter linked to the trfA gene.\u003cbr\u003eHigh transformation efficiency with clones of all sizes.\u003cbr\u003elacZΔM15 for blue\/white screening of recombinants.\u003cbr\u003eRestriction-minus [mcrA, Δ(mrr-hsdRMS-mcrBC)] phenotype enables efficient cloning of methylated DNA.\u003cbr\u003eEndonuclease-minus (endA1), to ensure high yields of DNA.\u003cbr\u003eRecombination-minus (recA1), for greater stability of large insert clones.\u003c\/p\u003e\u003cp\u003eApplications\u003c\/p\u003e\u003cp\u003eGeneration of inducible copy-number clones using the CopyControl™ Cloning System.\u003c\/p\u003e\u003cp\u003eFigure 1. Copy number of CopyControl™ BAC clones can be induced 10- to 20-fold in TransforMax™ EPI300™ E. coli. The yield of BAC DNA from CopyControl BAC clones of 110-145 kb increased \u0026gt;14-fold following addition of CopyControl Induction Solution. U=uninduced cells; I=induced cells.\u003c\/p\u003e\u003cp\u003eGenotype\u003c\/p\u003e\u003cp\u003eF- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL (StrR) nupG trfA dhfr\u003c\/p\u003e\u003cp\u003eTransforMax EPI300 Electrocompetent E. coli\u003c\/p\u003e\u003cp\u003eTransformation efficiency of \u0026gt;1 x 1010 cfu\/µg of pUC19.\u003c\/p\u003e\u003cp\u003eTransforMax EPI300 Chemically Competent E. coli\u003c\/p\u003e\u003cp\u003eTransformation efficiency of \u0026gt;5 x 108 cfu\/µg of pUC19.\u003c\/p\u003e\u003cp\u003e*Covered by issued and\/or pending patents.\u003c\/p\u003e\u003cp\u003eread more\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eStandard cloning for large inserts and Gibson assembly electroporation\u003c\/li\u003e\n\u003cli\u003eClone large inserts and transform large plasmids - up to at least 145 kb plasmid DNA.\u003c\/li\u003e\n\u003cli\u003eGenerate clones with inducible copy number using CopyControlvect ors.\u003c\/li\u003e\n\u003cli\u003eAchieve high transformation efficiencies.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003e1,000X concentrated solution. Filter sterilised.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_COMCEL-009_C300C105-TransforMax-EPI300-Chemically-Competent-E-coli\"\u003eC300C105 TransforMax EPI300 Chemically Competent E coli\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_COMCEL-009_CopyControl-Induction-Soln\"\u003eCopyControl Induction Soln\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_COMCEL-009_TMax-EPI300-cells\"\u003eTMax EPI300 cells\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"10 Reactions","offer_id":45986439299151,"sku":"C300C105","price":1126.0,"currency_code":"ILS","in_stock":true},{"title":"20 Reactions","offer_id":45986439331919,"sku":"EC300110","price":2322.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TransforMax-EPI300_33b8a28e-d1b9-4509-9eb7-444a6761b352.jpg?v=1785956183"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/collections\/biosearch-logo.svg?v=1780493985","url":"https:\/\/www.danyel.co.il\/collections\/biosearch-technologies.oembed","provider":"danyel biotech","version":"1.0","type":"link"}